Journal of Applied Spectroscopy

, Volume 81, Issue 3, pp 360–364

Spectral Properties of the Anionic Form of the Molecular Probe Fet


The relationship of the fluorescence spectral characteristics of the anionic form of the molecular probe 4′-(diethylamino)-3-hydroxyflavone (FET) to intramolecular proton transfer in a weakly polar solvent (CH2Cl2) was studied. The fluorescence spectrum of this form had a broad featureless band in the area between the shortwavelength and long-wavelength fluorescence bands of FET. Its excitation spectrum had strong UV maxima (350 and 305 nm). This fluorescence disappeared completely if the solution was cooled to 1 °C. The deprotonation mechanism of neutral FET molecules was discussed.


fluorescence proton transfer anionic form probe 


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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  1. 1.Institute of Physics, Pomeranian University in SlupskSlupskPoland

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