The binding of lotus seedpod oligomeric procyanidins (LSOPC) and catechin (a major constituent unit of LSOPC) to bovine serum albumin (BSA) was studied by a fluorescence quenching technique. The results revealed that LSOPC could strongly quench the intrinsic fluorescence of BSA through a static quenching procedure, but catechin could not. The Stern–Volmer quenching constant, K SV, and corresponding thermodynamic parameters, ΔG 0, ΔH 0 and ΔS 0, were calculated. The results of synchronous fluorescence and circular dichroism studies showed that LSOPC could cause a conformational change in BSA. In addition, glucose and metal ions could affect the interaction between LSOPC and BSA.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
J. Flarakos, K. L. Morand, P. Vouros, Anal. Chem., 77, 1345–1353 (2005).
D. C. Carter, J. X. Ho, Adv. Protein Chem., 45, 153–203 (1994).
A. Zhang, L. Jia, Spectrosc. Lett., 39, 285–298 (2006).
K. F. Brown, M. J. Crooks, Biochem. Pharmacol., 25, 1175–1178 (1976).
S. Ashoka, J. Seetharamappa, P. B. Kandagal, J. Lumin., 121, 179–186 (2006).
X. L. Han, P. Mei, Y. Liu, Spectrochim. Acta, A, 74, 781–787 (2009).
R. B. Gregory, M. Gaugoda, R. K. Gilpin, W. Su, Biopolymers, 33, 1871–1876 (1993).
V. Bhakuni, C. M. Gupta, FEBS Lett., 205, 347–350 (1986).
E. Shechter, E. R. Blout, Proc. Natl. Acad. Sci. USA, 51, 670—675 (1964).
W. Lindner, J. Chromatogr. B, 677, 1–28 (1996).
D. E. Epps, T. J. Raub, V. Caiolfa, J. Pharm. Pharmacol., 51, 41–48 (1998).
T. F. Jiang, Y. H. Wang, Z. H. Lv, J. Pharm. Biomed. Anal., 43, 854–858 (2007).
M. Leopoldini, N. Russo, M. Toscano, J. Agr. Food Chem., 55, 7944–7949 (2007).
H. Qu, Y. Ma, K. Yu, J. Pharm. Biomed. Anal., 43, 66–72 (2007).
Y. S. Gong, Y. Tang, J. S. Xiong, B. J. Xie, Acta Nutrimenta Sinica, 28, 318–321 (2010).
D. W. Boulton, U. K. Walle, T. Walle, J. Pharm. Pharmacol., 50, 243–249 (1998).
J. Liu, J. Tian, W. He, J. Pharm. Biomed. Anal., 35, 671–677 (2004).
Y. Q. Wang, H. M. Zhang, G. C. Zhang, J. Lumin., 126, 211–218 (2007).
S. Bi, L. Ding, Y. Tian, J. Mol. Struct., 703, 37–45 (2004).
W. G. Cao, Q. C. Jiao, Y. Fu, Spectrosc. Lett., 36, 197–209 (2003).
R. K. Nanda, N. Sarkar, R. Banerjee, J. Photochem. Photobiol. A, 192, 152–158 (2007).
T. Yuan, A. M. Weljie, H. J. Vogel, Biochemistry, 37, 3187–3195 (1998).
J. Chen, X. Y. Jiang, X. Q. Chen, J. Mol. Struct., 876, 121–126 (2008).
Y. Z. Zhang, B. Zhou, X. P. Zhang, J. Hazard. Mater., 163, 1345–1352 (2009).
G. Z. Chen, X. Z. Huang, J. G. Xu, Z. Z. Zheng, Z. B. Wang, Beijing Science Press, 2–112 (1990).
J. R. Lakowicz, G. Weber, Biochemistry, 12, 4161–4170 (1973).
S. Bi, D. Song, Y. Tian, Spectrochim. Acta, A, 61, 629–636 (2005).
D. Leckband, Annu. Rev. Biophys. Biom., 29, 1–26 (2000).
P. D. Ross, Biochemistry, 20, 3096–3102 (1981).
G. Sudlow, D. J. Birkett, D. N. Wade, Mol. Pharmacol., 12, 1052–1061 (1976).
Q. Zhang, Y. Ni, S. Kokot, Spectrosc. Lett., 45, 85–92 (2012).
Z. H. Wang, Z. D. Sun, B. J. Xie, Food Sci., 32, 77–82 (2011).
H. Liang, Y. Q. Zhou, P. W. Shen, Chin. Sci. Bull., 39, 1452–1457 (1994).
H. Gao, L. Lei, J. Liu, Q. Kong, X. Chen, Z. Hu, J. Photochem. Photobiol. A, 167, 213–221 (2004).
U. Katrahalli, S. Jaldappagari, S. S. Kalanur, J. Lumin., 130, 211–216 (2010).
Author information
Authors and Affiliations
Corresponding author
Additional information
Published in Zhurnal Prikladnoi Spektroskopii, Vol. 80, No. 6, pp. 893–900, November–December, 2013.
Rights and permissions
About this article
Cite this article
Wu, Q., Li, S., Fu, X. et al. Spectroscopic Studies on Binding of Lotus Seedpod Oligomeric Procyanidins to Bovine Serum Albumin. J Appl Spectrosc 80, 884–892 (2014). https://doi.org/10.1007/s10812-014-9860-6
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10812-014-9860-6