We have used the IR absorption spectra of blood, plasma, and packed erythrocytes to study the effect of in vivo exposure of blood to low-intensity emission from a He–Ne laser (λ = 632.8 nm, 4 mW/cm2) on the molecular components of blood. In comparing the IR spectra of samples of blood sample and blood components before and after irradiation, we identified changes in the amide A, amide I, amide II, amide III absorption regions and also in the absorption bands of methyl, methylene, and phosphate groups. We conclude that exposure of blood in vivo to emission from a He–Ne laser leads to a decrease in the contribution of the α-helix conformation in the secondary structure of the blood proteins.
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Translated from Zhurnal Prikladnoi Spektroskopii, Vol. 76, No. 5, pp. 760–767, September–October, 2009.
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Zalesskaya, G.A., Kalosha, I.I. Effect of in vivo irradiation of blood by low-intensity emission from a He–Ne laser on molecular components of blood. J Appl Spectrosc 76, 720–726 (2009). https://doi.org/10.1007/s10812-009-9246-3
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DOI: https://doi.org/10.1007/s10812-009-9246-3