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High voltage pulsed electric field and electroporation technologies for algal biomass processing

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Abstract

The production of animal and terrestrial plant proteins has environmental impacts that could limit their sustainable supply. Proteins from microalgae and macroalgae can support the growing animal-free protein demand sustainably, using non-arable land and seawater. However, current technologies and processes developed for terrestrial plant protein processing have multiple limitations when applied to micro and macroalgae, thus, providing an opportunity for emerging technologies. Here we critically review various methods used for micro and macroalgal cell disruption for protein extraction. The pulsed electric field (PEF) algal biomass process is focused on further and highlighted as a green approach for protein extraction from algae. The potential of high-voltage pulsed electric fields and electroporation technologies for algal protein processing are discussed in detail. Current state of the art, challenges, and perspectives are also discussed. The current review will aid in establishing PEF for processing algal biomass to extract valuable compounds including proteins using a non-thermal and chemical-free approach, thus providing an environmentally friendly solution for protein extraction from algae along with highlighting upscaling and processing challenges.

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Acknowledgements

The authors thank Porter School of Earth and Environmental Sciences, Tel Aviv University, Israel, and Good Food Institute, Israel, for the motivation and facilities.

Funding

Mrinal Kashyap will like to thank the sandwich VATA fellowship funded by Council for higher education, Israel.

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Authors and Affiliations

  1. Porter School of Earth and Environmental Sciences, Tel Aviv University, Tel Aviv, Israel

    Mrinal Kashyap & Alexander Golberg

  2. Birla Institute of Technology & Science Pilani - Hyderabad Campus, Secunderabad, India

    Supratim Ghosh

  3. Department of Biosciences and Biomedical Engineering, Indian Institute of Technology Indore, Indore, India

    Kiran Bala

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  1. Mrinal Kashyap
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  2. Supratim Ghosh
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  3. Kiran Bala
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  4. Alexander Golberg
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Contributions

Mrinal Kashyap: conceptualization, writing-original draft. Supratim Ghosh: writing, reviewing, and editing the manuscript draft. Alexander Golberg: supervised the conceptualization, funding, writing, reviewing, and editing. Kiran Bala: contributed by supervising, revising, and editing the manuscript.

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Correspondence to Mrinal Kashyap or Alexander Golberg.

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Competing interests

Alexander Golberg has patent applications on devices using pulsed electric field technologies for seaweed processing with Ramot, Tel Aviv University. Alexander Golberg has a financial interest in Genesea Advanced Technologies Ltd, which focuses on seaweed protein production.

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Kashyap, M., Ghosh, S., Bala, K. et al. High voltage pulsed electric field and electroporation technologies for algal biomass processing. J Appl Phycol 36, 273–289 (2024). https://doi.org/10.1007/s10811-023-03145-2

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