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Influence of a static magnetic field on the photosynthetic apparatus, cell division, and biomass composition of a Chlorella microalgae-bacteria consortium

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Abstract

The main objective of this research was to evaluate the effect of a 47 mT static magnetic field (SMF) applied at different times of the exponential growth phase of a Chlorella microalgae-bacteria consortium. For this, growth parameters, cell division, the photochemical activity of photosystem II (PS II), and the biochemical composition of the microorganisms were studied. Biomass concentration and productivity of cultures exposed to SMF increased concerning control cultures, reaching maximum values when this physical agent was applied in the early exponential phase, 0.89 g L−1 and 0.075 g L−1 d−1, respectively. In addition, SMF application stimulated binary and multiple cell division of cultures exposed during early and late exponential phases. PS II quantum yield was significantly increased over control cultures immediately after applying SMF during early (0.70) and late (0.73) exponential phases. In addition, in cultures exposed to SMF, the quantum yield for electron transport (ϕEo) increased, and the absorption flux per reaction center (ABS/RC) decreased, which was associated with an increase in the active reaction centers of PS II. Extracellular protein, carbohydrate, and polysaccharide content varied when SMF was applied during the early exponential phase. No significant differences were observed regarding the lipid content of the control cultures and those exposed to SMF. It is concluded that SMF increases the formation of radical pairs in photosystem II due to the increase in the number of active reaction centers, which could constitute the mechanism of action of this system.

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Data availability

The datasets generated during and/or analysed during the current study are available from the corresponding author on reasonable request.

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Funding

The authors are thankful for the financial support from Project 1 and Project 5 of the Programme VLIR-IUC-UOS – Universidad de Oriente CU2019IUC030A105-77143 and the UHasselt BOF-BILA fellowship R-9903.

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Authors and Affiliations

  1. Centro Nacional de Electromagnetismo Aplicado, Universidad de Oriente, Santiago de Cuba, Cuba

    Yadira Silveira Font, Yadenis Ortega Díaz & Elizabeth Isaac Alemán

  2. Environmental Biology, Centre for Environmental Sciences, Hasselt University, B-3590, Diepenbeek, Belgium

    Ann Cuypers

  3. Analytical and Circular Chemistry, CMK, IMO, Hasselt University, B-3590, Diepenbeek, Belgium

    Dries Vandamme

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  1. Yadira Silveira Font
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  2. Yadenis Ortega Díaz
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  3. Ann Cuypers
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  4. Elizabeth Isaac Alemán
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  5. Dries Vandamme
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Contributions

Y.S. Font: Conceptualization, Investigation, validation, visualization, writing-original Draft Y.O. Díaz: Conceptualization, writing – Review and editing A. Cuypers: Conceptualization, Resources, Writing – Review and Editing, funding acquisition, E.I Alemán: writing – review and editing, validation, funding acquisition, supervision; D. Vandamme: conceptualization, resources, writing – Review and editing – supervision – funding acquisition.

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Correspondence to Dries Vandamme.

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Silveira Font, Y., Ortega Díaz, Y., Cuypers, A. et al. Influence of a static magnetic field on the photosynthetic apparatus, cell division, and biomass composition of a Chlorella microalgae-bacteria consortium. J Appl Phycol 36, 41–56 (2024). https://doi.org/10.1007/s10811-023-03137-2

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