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Effects of six phytohormones on the growth behavior and cellular biochemical components of Chlorella vulgaris 31

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Abstract

Microalgae possess similar characteristics to higher plants and research has shown that phytohormones can influence the metabolic and physiological properties of microalgae. Trace amounts of phytohormones can affect the production performance of microalgae, which can be used to increase the yield of specific components of microalgae, elevate the position of microalgae in the industrial raw material supply chain, promote green production, and reduce carbon emissions. This study determined the growth and physiological and biochemical composition of Chlorella vulgaris 31 (Cv-31) after 12 days of cultivation under treatment with different concentrations of six phytohormones, 6-benzyladenine (6-BA), ethylene (ETH), gibberellin (GA), indoleacetic acid (IAA), naphthaleneacetic acid (NAA), and salicylic acid (SA). Different concentrations and types of phytohormones had a significant effect on the growth and biochemical composition of Cv-31. GA at 10 mg L−1 effectively promoted the growth and pigment accumulation of Cv-31, while SA at 7.5 mg L−1 increased lipid accumulation by 3.3 times compared to the control. ETH and GA contributed to the accumulation of soluble sugars, but the six phytohormones had a limited effect on the protein content of Cv-31. This study comprehensively examined the effects of six phytohormones at different concentrations on the growth and main components of Cv-31, providing a reference for related research and production.

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Funding

This work was supported by Higher Education Industry Support Plan Project of Gansu Province, China (2020C-21; 2021CYZC-37); Key Research and Development Project of Gansu Province (22YF7NA118); Incubation Programme for Key Scientific Research Project of Northwest Normal University (NWNU-LKZD2022-02).

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Authors and Affiliations

  1. College of Life Sciences, Northwest Normal University, No. 967 Anning East Road, Lanzhou, 730070, China

    Zijian Xie, Saimai Ma, Yueqin Cao, Shuting Peng, Xiaoyun Zhang & Weibao Kong

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  1. Zijian Xie
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  2. Saimai Ma
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  3. Yueqin Cao
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  4. Shuting Peng
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  5. Xiaoyun Zhang
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Contributions

Zijian Xie: Methodology, Formal analysis, Investigation, Data Curation, Writing -Original Draft, Writing—Review & Editing, Visualization

Saimai Ma: Methodology, Validation, Investigation, Writing—Review & Editing

Yueqin Cao: Methodology, Validation, Formal analysis, Investigation, Writing -Original Draft

Shuting Peng: Methodology, Investigation, Data Curation, Writing—Review & Editing

Xiaoyun Zhang: Methodology, Data Curation, Writing—Review & Editing

Weibao Kong: Conceptualization, Methodology, Resources, Supervision, Project administration, Funding acquisition

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Correspondence to Weibao Kong.

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Xie, Z., Ma, S., Cao, Y. et al. Effects of six phytohormones on the growth behavior and cellular biochemical components of Chlorella vulgaris 31. J Appl Phycol 35, 1589–1602 (2023). https://doi.org/10.1007/s10811-023-03010-2

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