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Static magnetic field improved growth and astaxanthin production in Haematococcus lacustris via the regulation of carbohydrate accumulation, H2O2 level, and antioxidant defense system

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Abstract

This study assessed a culture system using a static magnetic field (SMF) for improving biomass and astaxanthin production in Haematococcus lacustris. Different intensities of SMF (0, 2, 4, and 8 mT) were applied to H. lacustris cell suspension culture at the beginning of the logarithmic phase, and then cell growth, antioxidant compounds, and oxidative damage were analyzed after 12 days. SMF at 8 mT caused a significant increase in cell growth, chlorophyll (a and b) concentration, total carbohydrate and protein contents, and antioxidant enzyme activities, including superoxide dismutase (SOD), catalase (CAT), and ascorbate peroxidase. Maximum astaxanthin accumulation (21.05 mg g−1, 4.9 mg L−1 dry weight) was identified at 4 mT, which was 1.58 times compared to control. SMF increased significantly hydrogen peroxide (H2O2) level and decreased malondialdehyde content, especially at 4 mT. Electrophoretic patterns displayed Mn-SOD, and CAT1 isoforms were the main isoforms to scavenge the ROS in H. lacustris cells. Moreover, SMF significantly enhanced total phenol, flavonoid, and DPPH scavenging activity. SMF-treated H. lacustris extracts showed an antibacterial potential against Staphylococcus aureus. These results provided an efficient method for improving growth and astaxanthin production and displayed the biochemical mechanism underlying secondary metabolite production in H. lacustris.

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Change history

  • 29 August 2022

    The affiliation of Negar Korshidi and Hakimeh Ziyadi has been changed.

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Acknowledgements

Authors thank the Paya Kesht Company for providing the SMF-generating device.

Funding

The author thanks the Islamic Azad University for financial support.

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Authors and Affiliations

  1. Department of Organic Chemistry, Faculty of Pharmaceutical Chemistry, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran

    Negar Khorshidi & Hakimeh Ziyadi

  2. Aerospace Research Institute, Ministry of Science Research and Technology, Tehran, 14665-834, Iran

    Halimeh Hassanpour

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  1. Negar Khorshidi
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  2. Halimeh Hassanpour
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Negar Khorshidi, conducted the bench experiments. Dr. Halimeh Hassanpour designed and supervised the entire work and participated in some experiments. Dr. Hakimeh Ziyadi performed the necessary statistical analysis. All authors read and approved the manuscript.

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Correspondence to Halimeh Hassanpour.

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Khorshidi, N., Hassanpour, H. & Ziyadi, H. Static magnetic field improved growth and astaxanthin production in Haematococcus lacustris via the regulation of carbohydrate accumulation, H2O2 level, and antioxidant defense system. J Appl Phycol 34, 2283–2295 (2022). https://doi.org/10.1007/s10811-022-02758-3

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