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Short-term variations of mycosporine-like amino acids in the carrageenan-producing red macroalga Mazzaella laminarioides (Gigartinales, Rhodophyta) are related to nitrate availability

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Abstract

The effect of solar UV radiation exposure and NO3– supply on mycosporine-like amino acids (MAAs) accumulation in the carrageenan-producing red macroalga Mazzaella laminarioides in a short-term period was studied. Apical segments of M. laminarioides tetrasporophytes were exposed to three treatments of solar radiation (PAR [P], PAR+UV-A [PA], and PAR+UV-A+UV-B [PAB]) under 0.38 mM NO3– or without NO3– supply. Samples were taken at the following local times: 8:00 (initial value), 13:00, and 18:00, and at 8:00 on the following day. Short-term variations in total MAA content were observed, being this variation accounted by the interaction of radiation, time exposure, and NO3– treatments. The highest MAAs content (above 3 mg g−1 DW) was observed at 13:00 under PA (without NO3−) and PAB (with NO3–), and at 18:00 under PA (with NO3–) and PAB (with NO3–) treatments. Four MAAs were identified: asterina-330, shinorine, palythine, and mycosporine-glycine. The content and percentage of each MAA varied during the experimental time. While the accumulation of shinorine and palythine varied according to NO3– supply, mycosporine-glycine did it according to the interaction of time exposure and radiation treatment. The short-term (hours) variations in total MAAs content and the concentration of each MAA as a response to the high solar irradiance received could be a prerequisite in intertidal species to thrive in a highly variable environment.

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Funding

The study was funded by grants from ANID-Chile: Fondecyt Iniciación No. 11160520 and Centro Fondap-IDEAL 15150003.

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Authors and Affiliations

  1. Laboratorio de Ecofisiología y Biotecnología de Algas (LEBA), Facultad de Ciencias, Universidad de Magallanes, Punta Arenas, Chile

    NP Navarro & J Jofre

  2. Centro FONDAP de Investigación en Dinámica de Ecosistemas Marinos de Altas Latitudes (IDEAL), Punta Arenas, Chile

    NP Navarro

  3. Network for Extreme Environments Research, Universidad de Magallanes, Punta Arenas, Chile

    NP Navarro

  4. Universidad de Málaga, Instituto Universitario de Biotecnología y Desarrollo Azul (IBYDA), Departamento de Ecología, Facultad de Ciencias, Campus Universitario de Teatinos s/n, 29071, Málaga, Spain

    N Korbee & FL Figueroa

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Contributions

Nelso P Navarro: Conceptualization, data curation, formal analysis, investigation, writing, and funding acquisition. Nathalie Korbee: Conceptualization, formal analysis, writing—reviewing, and editing. Jocelyn Jofre: Investigation and data acquisition, data curation, project administration. Félix López Figueroa: Conceptualization, formal analysis, investigation, writing—reviewing, and editing. All authors read and approved the final manuscript.

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Navarro, N., Korbee, N., Jofre, J. et al. Short-term variations of mycosporine-like amino acids in the carrageenan-producing red macroalga Mazzaella laminarioides (Gigartinales, Rhodophyta) are related to nitrate availability. J Appl Phycol 33, 2537–2546 (2021). https://doi.org/10.1007/s10811-021-02452-w

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