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Effects of light intensity on the epiphytic bacterial community of sporelings of Saccharina japonica

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Abstract

In the artificial hatchery of Saccharina japonica, sporelings suffer from various diseases caused by improper irradiance. On the other hand, the outbreaks of diseases of algae are also related to the changes of epiphytic bacteria. So far, little is known about the effects of light intensity on composition shifts of the epiphytic bacterial community of sporelings in S. japonica. In this study, we analyzed the effect of light intensity on the dynamic shift of diversity, the composition of bacterial communities on sporelings of S. japonica by using high-throughput sequencing based on 16S rRNA genes. The sporelings were exposed to different light intensities ranging from 0 to 200 μmol photons m−2 s−1 for a week. Our results showed that the composition of the epiphytic bacterial community shifted and the diversity increased when the light intensity was elevated. At the phylum level, the phylum Bacteroidetes showed increasing trends while phylum Proteobacteria decreased. At the genus level, Leucothrix and Colwellia were dominant in all treatment groups and the genus Leucothrix decreased in abundance while the genus Colwellia increased, as the light intensity was enhanced. The results of this study not only enrich the baseline knowledge of epiphytic bacteria associated with S. japonica but also help hatchery farms to take appropriate light management and to monitor the shift of epiphytic bacterial community which could mitigate disease outbreaks.

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Acknowledgements

We would like to thank Dong Miu for the technical assistance and Fan Yang for the advice on data analysis.

Funding

Financial support came from the National Natural Science Foundation of China (No. 31702365), China Agriculture Research System (CARS-50), the National Key Technology Support Program (2015BAD13B05), the Biological Resources Programme of the Chinese Academy of Sciences (CZBZX-1, KFJ-BRP-017-27), the National Marine Genetic Resource Center, the Taishan Scholar Program of Shandong Province, and the Huiquan Scholar Program of Institute of Oceanology, Chinese Academy of Sciences.

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Contributions

JL and SJP designed the study and wrote the manuscript. JL and QXL analyzed the sequencing data. LS helped in culturing the sporelings. All authors approved the final manuscript.

Corresponding author

Correspondence to Shaojun Pang.

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The authors declare no competing interests.

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Li, J., Li, Q., Su, L. et al. Effects of light intensity on the epiphytic bacterial community of sporelings of Saccharina japonica. J Appl Phycol 33, 1759–1764 (2021). https://doi.org/10.1007/s10811-021-02406-2

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Keywords

  • Epiphytic bacterial community
  • Light intensity
  • 16S RNA gene amplicon sequencing
  • Saccharina japonica
  • Phaeophyceae