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In vitro antifungal activity screening of beach-cast seaweeds collected in Yucatan, Mexico

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Abstract

The use of natural products in fungal disease control is an approach to reduce the negative impact of synthetic fungicides in agriculture and the environment. Although there are many alternatives, beach-cast seaweeds are a natural source of novel compounds that can be useful against fungal phytopathogens. In this study, aqueous extracts (AEs) of five species of beach-cast seaweeds, Agardhiella subulata, Bryothamnion triquetrum, Codium isthmocladum, Dictyota dichotoma, and Halymenia floresii, collected in Yucatan, Mexico, were tested against Fusarium oxysporum, Colletotrichum gloeosporioides, and Pseudocercospora fijiensis by dilution assays. From these, only H. floresii AE showed antifungal activity against P. fijiensis (13.7 mg mL−1). Furthermore, the protein fraction isolated from this AE was also active against P. fijiensis at 0.625 μg mL−1. The chromatographic profile of H. floresii AE with antifungal activity showed three major peaks at retention times (tR) 1.6, 23.0, and 23.9 min, respectively. Analysis of solids in H. floresii AE after lyophilization revealed a possibly sulfated polysaccharide (carrageenan) derivative. The toxicity analysis on Artemia salina showed that this aqueous extract is safe.

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Acknowledgments

We would like to thank Dr. Ileana Ortegón-Aznar for confirming the identification of the species; Felipe Barredo-Pool, Silvia Hernández-Aguilar, and Roger Sulub-Tun, for their technical support. Also, thanks to National Council for Science and Technology (CONACYT), for the scholarship (# 612342) of Mauricio Gómez-Hernández.

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Authors and Affiliations

  1. Unidad de Biotecnología, Centro de Investigación Científica de Yucatán, Calle 43 #130, Col. Chuburná de Hidalgo, C.P. 97205, Mérida, Yucatán, México

    Mauricio Gómez-Hernández, Cecilia Mónica Rodríguez-García, Leticia Peraza-Echeverría, Sergio Rubén Peraza-Sánchez & Luis Wiliunfo Torres-Tapia

  2. GEMBIO, Centro de Investigación Científica de Yucatán, Mérida, Yucatán, México

    Daisy Pérez-Brito

  3. Unidad de Materiales, Centro de Investigación Científica de Yucatán, Mérida, Yucatán, México

    Rossana Faride Vargas-Coronado & Juan Valerio Cauich-Rodríguez

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  1. Mauricio Gómez-Hernández
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  2. Cecilia Mónica Rodríguez-García
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  3. Leticia Peraza-Echeverría
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Correspondence to Cecilia Mónica Rodríguez-García.

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Gómez-Hernández, M., Rodríguez-García, C.M., Peraza-Echeverría, L. et al. In vitro antifungal activity screening of beach-cast seaweeds collected in Yucatan, Mexico. J Appl Phycol 33, 1229–1237 (2021). https://doi.org/10.1007/s10811-021-02384-5

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