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Effects of extracted polysaccharides from a Chlorella vulgaris biomass on expression of interferon-γ and interleukin-2 in chicken peripheral blood mononuclear cells

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Abstract

Polysaccharides isolated from algae species have been shown to have various biological properties including immunomodulatory activities. In the present study, the effects of water-extractable polysaccharides from a Chlorella vulgaris biomass on the expression of immunostimulatory cytokines by chicken peripheral blood mononuclear cells (PBMCs) were evaluated. Extracted polysaccharides were fractionated using a DEAE Sepharose FF column yielding two fractions (F1 and F2). Crude polysaccharide (CP) and fractions mostly consisted of carbohydrates (71.9 to 82.9%), protein (6 to 13.8%), and uronic acids (4.8 to 7.9%). Monosaccharide constituents were predominantly galactose (13.1 to 53.2%), glucose (15.3 to 38.6%), mannose (5.5 to 11.2%), arabinose (5.6 to 20.7%), and rhamnose (5.8 to 14.7%). Crude, F1, and F2 polysaccharides contained 1 or 2 sub-fractions with average molecular weight ranging from 19.2 to 161.1 × 103 g mol−1. Evaluation of immunostimulatory activities of CP and fractions (200 to 1000 μg mL−1) revealed significant effects on PBMC interferon-γ (IFN-γ) and interleukin-2 (IL-2) expression. The most potent immunostimulating fraction F1 was consisted of a homogeneous polysaccharide with relatively low molecular weight (23.9 × 103 g mol−1), low structural compactness, and mixed linkages of (→1)-, (1→3)-, (1→3,6)-galactopyranose and -glucopyranose residues.

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Funding

This work was supported by Iran National Science Foundation (INSF).

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Authors and Affiliations

  1. Department of Agriculture, Iranian Research Organization for Science and Technology (IROST), Ehsanirad Street, Tehran, 3313193685, Iran

    Sara Mirzaie

  2. Department of Seafood Processing, Faculty of Marine Sciences, Tarbiat Modares University, P.O. Box: 46414-356, Noor, Iran

    Mahdi Tabarsa

  3. Department of Biotechnology, Iranian Research Organization for Science and Technology (IROST), Tehran, 3313193685, Iran

    Maliheh Safavi

Authors
  1. Sara Mirzaie
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  2. Mahdi Tabarsa
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  3. Maliheh Safavi
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Contributions

Sara Mirzaie: conceptualization, methodology, investigation, writing original draft. Mahdi Tabarsa: methodology; writing, review, and editing; resources. Maliheh Safavi: investigation, resources

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Correspondence to Sara Mirzaie.

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The authors declare that they have no conflict of interest.

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Animal procedure was approved by the institutional Animal Care Committee of the Department of Agriculture, Iranian Research Organization for Science and Technology.

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Mirzaie, S., Tabarsa, M. & Safavi, M. Effects of extracted polysaccharides from a Chlorella vulgaris biomass on expression of interferon-γ and interleukin-2 in chicken peripheral blood mononuclear cells. J Appl Phycol 33, 409–418 (2021). https://doi.org/10.1007/s10811-020-02301-2

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  • DOI: https://doi.org/10.1007/s10811-020-02301-2

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