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Application of Jerusalem artichoke and lipid-extracted algae hydrolysate for docosahexaenoic acid production by Aurantiochytrium sp. KRS101

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Abstract

Microalgae-derived docosahexaenoic acid (DHA) production has been considered an alternative technology to overcome several disadvantages of fish oil-based DHA production. Despite its potential, efficient production methods for DHA have been rarely reported. Accordingly, in this study we investigated the potential application of Jerusalem artichoke and lipid-extracted algae (LEA) hydrolysates as inexpensive sources of carbon and other nutrients for DHA production using Aurantiochytrium sp. KRS101. Response surface methodology (RSM) with central composite design (CCD) was used to determine the maximum point of DHA productivity in the presence of 51.9 g L−1 fructose and 4.9 g L−1 yeast extract. Hydrolysis of 76.6 g L−1 Jerusalem artichoke powder with 0.25 N sulfuric acid at 70 °C yielded the desired concentration of fructose for use as the medium. Cultivation of the microalgae using the Jerusalem artichoke hydrolysate (JAH) and JAH + LEA (JLH-1) resulted in 27.8% and 31.3% improvements in biomass productivity, respectively, compared with the basal medium control. In addition, simultaneous one-step hydrolysis of Jerusalem artichoke and LEA (JLH-2) resulted in 7.2% higher biomass and 47% higher DHA yields than that obtained from hydrolyzing the two components separately (JLH-1). These results suggested that a strategy using JAH combined with LEA could replace expensive nutrient sources and produce efficient and eco-friendly DHA from Aurantiochytrium sp. KRS101.

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Funding

This research was supported by the Advanced Biomass R&D Center (ABC) of the Global Frontier Project, funded by the Ministry of Science and ICT (grant no. ABC-2010-0029728), and a grant from the Nakdonggang National Institute of Biological Resources (NNIBR), funded by the Ministry of Environment (MOE) of the Republic of Korea (grant no. NNIBR202002101).

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Author notes

  1. Sung-Woon Heo

    Present address: LG Chem, 188 Munji-ro, Yuseong-gu, Daejeon, 34122, Republic of Korea

  2. Sung-Woon Heo and Young Taek Oh contributed equally to this work.

Authors and Affiliations

  1. Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science & Technology (KAIST), 291 Daehak-ro, Yuseong-gu, Daejeon, 34141, Republic of Korea

    Sung-Woon Heo & Yong Keun Chang

  2. Department of Animal and Plant Research, Nakdonggang National Institute of Biological Resources (NNIBR), SangJu-si, 37242, South Korea

    Young Taek Oh

  3. Microbial Research Department, Nakdonggang National Institute of Biological Resources (NNIBR), SangJu-si, 37242, South Korea

    Z-Hun Kim

  4. Advanced Biomass R&D Center, Korea Advanced Institute of Science & Technology (KAIST), 291 Daehak-ro, Yuseong-gu, Daejeon, 34141, Republic of Korea

    Yong Keun Chang

  5. Department of Microbiology and Resources, College of Science and Technology, Mokwon University, 88 Doanbuk-ro, Seo-Gu, Daejeon, 35349, Republic of Korea

    Bongsoo Lee

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  1. Sung-Woon Heo
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Correspondence to Yong Keun Chang or Bongsoo Lee.

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Heo, SW., Oh, Y.T., Kim, ZH. et al. Application of Jerusalem artichoke and lipid-extracted algae hydrolysate for docosahexaenoic acid production by Aurantiochytrium sp. KRS101. J Appl Phycol 32, 3655–3666 (2020). https://doi.org/10.1007/s10811-020-02207-z

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