Abstract
Hydrogen production from microalgae has attracted considerable attention due to its high energy content and as a renewable and environmentally friendly energy source. Various strains of microalgae have been reported to produce “biohydrogen”, but screening for new strains is still necessary to discover strains with higher hydrogen yields. A newly isolated hydrogen-producing green alga was screened and labeled Chlorella sp. KLSc59. The effect of extracellular pH, light intensity, external carbon sources, reducing agents, and nutrient deprivation on biohydrogen production of Chlorella sp. KLSc59 were investigated. Hydrogen yield was higher under anaerobic conditions. Under external pH 7.2 with 53.2 μmol photons m−2 s−1 light intensity and using acetate as a carbon source, the optimum hydrogen yield was 281 μmol H2 mg−1 Chl. Nutrient deprivation reduced the hydrogen yield. Several reducing agents were assessed, and 1 mM ethanol enhanced yield by 3 times for 850 μmol H2 mg−1 Chl, and 1 mM sodium dithionite increased yield by 2.7 times for 750 μmol H2 mg−1 Chl. Significantly, our new strain showed higher hydrogen yields ranging from 1.5 to 68 times compared with other microalgae. Thus, Chlorella sp. KLSc59 showed valuable potential for biohydrogen production.
Similar content being viewed by others
References
Allakhverdiev S, Thavasi VK, Reslavski VD, Zharmukhamedov SK, Klimov VV, Ramakrishna S, Los DA, Mimuro MN, Ishihara H, Carpentier R (2010) Photosynthetic hydrogen production. J Photochem Photobiol C 11:101–113
Aparicio PJ, Azuara MP, Ballesteros A, Fernandez VM (1985) Effects of light intensity and oxidized nitrogen sources on hydrogen production by Chlamydomonas reinhardii. Plant Physiol 78:803–806
Batyrova KA, Tsygankov AA, Kosourov SN (2012) Sustained hydrogen photoproduction by phosphorus deprived Chlamydomonas reinhardtii cultures. Int J Hydrog Energy 37:8834–8839
Batyrova K, Gavrisheva A, Ivanova E, Liu JG, Tsygankov A (2015) Sustainable hydrogen photoproduction by phosphorus-deprived marine green microalgae Chlorella sp. Int J Mol Sci 16:2705–2716
Chia SR, Ong HC, Chew KW, Show PL, Phang SM, Ling TC, Nagarajan D, Lee DJ, Chang JS (2018) Sustainable approaches for algae utilisation in bioenergy production. Renew Energy 129:838–852
Gabrielyan L, Hakobyan L, Trchounian A (2017) Characterization of light-dependent hydrogen production by new green microalga Parachlorella kessleri in various conditions. J Photochem Photobiol B 175:207–210
Gibbs M, Gfeller RP, Chen C (1986) Fermentative metabolism of Chlamydomonas reinhardtii.III: photoassimilation of acetate. Plant Physiol 82:160–166
Han HL, Liu BQ, Yang HJ, Shen JQ (2012) Effect of carbon sources on the photobiological production of hydrogen using Rhodobacter sphaeroides RV. Int J Hydrog Energy 37:12167–12174
Juneja A, Ceballos RM, Murthy GS (2013) Effects of environmental factors and nutrient availability on the biochemical composition of algae for biofuels production: a review. Energies 6:4607–4638
Khosravitabar F (2020) Microalgal biohydrogen photoproduction: scaling up challenges and the ways forward. J Appl Phycol 32:277–289
Kosourov SN, Seibert M (2009) Hydrogen photoproduction by nutrient-deprived Chlamydomonas reinhardtii cells immobilized within thin alginate films under aerobic and anaerobic conditions. Biotechnol Bioeng 102:50–58
Kosourov S, Patrusheva E, Ghirardi ML, Seibert M, Tsygankov A (2007) A comparison of hydrogen photoproduction by sulfur-deprived Chlamydomonas reinhardtii under different growth conditions. J Biotechnol 128:776–787
Kruse O, Hankamer B (2010) Microalgal hydrogen production. Curr Opin Biotechnol 21:238–243
Laurinavichene T, Tolstygina I, Tsygankov A (2004) The effect of light intensity on hydrogen production by sulfur-deprived Chlamydomonas reinhardtii. J Biotechnol 114:143–151
Laurinavichene TV, Fedorov AS, Ghirardi ML, Seibert M, Tsygankov AA (2006) Demonstration of sustained hydrogen photoproduction by immobilized, sulfur-deprived Chlamydomonas reinhardtii cells. Int J Hydrog Energy 31:659–667
Lee YK, Shen H (2004) Basic culturing techniques. In: Richmond A (ed) Handbook of microalgal culture. Blackwell Science, Oxford, pp 40–56
Lee JY, Chen XJ, Lee EJ, Min KS (2012) Effects of pH and carbon sources on biohydrogen production by co-culture of Clostridium butyricum and Rhodobacter sphaeroides. J Microbiol Biotechnol 22:400–406
Lorencini P, Siqueira MR, Maniglia BC, Tapia DR, Maintinguer SI, Reginatto V (2016) Biohydrogen production from liquid and solid fractions of sugarcane bagasse after optimized pretreatment with hydrochloric acid. Waste Biomass Valoriz 7:1017–1029
Maneeruttanarungroj C, Phunpruch S (2017) Effect of pH on biohydrogen production in green alga Tetraspora sp. CU2551. Energy Procedia 138:1085–1092
Maneeruttanarungroj C, Lindblad P, Incharoensakdi A (2010) A newly isolated green alga, Tetraspora sp. CU2551, from Thailand with efficient hydrogen production. Int J Hydrog Energy 35:13193–13199
Maswanna T, Phunpruch S, Lindblad P, Maneeruttanarungroj C (2018) Enhanced hydrogen production by optimization of immobilized cells of the green alga Tetraspora sp CU2551 grown under anaerobic condition. Biomass Bioenergy 111:88–95
Melis A (2002) Green alga hydrogen production: progress, challenges and prospects. Intl J Hydrogen Energy 27:1217–1228
Melis A, Zhang L, Forestier M, Ghirardi ML, Seibert M (2000) Sustained photobiological hydrogen gas production upon reversible inactivation of oxygen evolution in the green alga Chlamydomonas reinhardtii. Plant Physiol 122:127–135
Meseck SL, Alix JH, Wikfors GH (2005) Photoperiod and light intensity effects on growth and utilization of nutrients by the aquaculture feed microalga, Tetraselmis chui (PLY429). Aquaculture 246:393–404
Oncel SVSF (2011) Effect of light intensity and the light: dark cycles on the long term hydrogen production of Chlamydomonas reinhardtii by batch cultures. Biomass Bioenergy 35:1066–1074
Papazi A, Gjindali AI, Kastanaki E, Assimakopoulos K, Stamatakis K, Kotzabasis K (2014) Potassium deficiency, a "smart" cellular switch for sustained high yield hydrogen production by the green alga Scenedesmus obliquus. Int J Hydrog Energy 39:19452–19464
Rashid N, Choi W, Lee K (2012) Optimization of two-staged bio-hydrogen production by immobilized Microcystis aeruginosa. Biomass Bioenergy 36:241–249
Sekoai PT, Kana EBG (2014) Semi-pilot scale production of hydrogen from organic fraction of solid municipal waste and electricity generation from process effluents. Biomass Bioenergy 60:156–163
Sekoai PT, Ayeni AO, Daramola MO (2019) Parametric optimization of biohydrogen production from potato waste and scale-up study using immobilized anaerobic mixed sludge. Waste Biomass Valoriz 10:1177–1189
Song W, Rashid N, Choi W, Lee K (2011) Biohydrogen production by immobilized Chlorella sp using cycles of oxygenic photosynthesis and anaerobiosis. Bioresour Technol 102:8676–8681
Stavropoulos KP, Kopsahelis A, Zafiri C, Kornaros M (2016) Effect of pH on continuous biohydrogen production from end-of-life dairy products (EoL-DPs) via dark fermentation. Waste Biomass Valoriz 7:753–764
Sunda W (1975) The relationship between cupric ion activity and the toxicity of copper to phytoplankton. PhD Thesis, Massachusetts Institute of Technology, USA
Touloupakis E, Rontogiannis G, Benavides AMS, Cicchi B, Ghanotakis DF, Torzillo G (2016) Hydrogen production by immobilized Synechocystis sp PCC 6803. Int J Hydrog Energy 41:15181–15186
Trchounian K, Müller N, Schink B, Trchounian A (2017) Glycerol and mixture of carbon sources conversion to hydrogen by Clostridium beijerinckii DSM791 and effects of various heavy metals on hydrogenase activity. Int J Hydrog Energy 42:7875–7882
Tsygankov AA, Kosourov SN, Tolstygina IV, Ghirardi ML, Seibert M (2006) Hydrogen production by sulfur-deprived Chlamydomonas reinhardtii under photoautotrophic conditions. Int J Hydrog Energy 31:1574–1584
Uyar B, Eroglu I, Yucel M, Gunduz U, Turker L (2007) Effect of light intensity, wavelength and illumination protocol on hydrogen production in photobioreactors. Int J Hydrog Energy 32:4670–4677
Wang X, Hao CB, Zhang F, Feng CP, Yang YN (2011) Inhibition of the growth of two blue-green algae species (Microsystis aruginosa and Anabaena spiroides) by acidification treatments using carbon dioxide. Bioresour Technol 102:5742–5748
Wei LZ, Yi J, Wang LJ, Huang TT, Gao FD, Wang QX, Ma WM (2017) Light intensity is important for hydrogen production in NaHSO3− treated Chlamydomonas reinhardtii. Plant Cell Physiol 58:451–457
Wutthithien P, Lindblad P, Incharoensakdi A (2019) Improvement of photobiological hydrogen production by suspended and immobilized cells of the N2-fixing cyanobacterium Fischerella muscicola TISTR 8215. J Appl Phycol 31:3527–3536
Xia A, Jacob A, Herrmann C, Murphy JD (2016) Fermentative bio-hydrogen production from galactose. Energy 96:346–354
Funding
This work was supported by the research grants by the Faculty of Science, King Mongkut’s Institute of Technology Ladkrabang to C. Maneeruttanarungroj (2562-01-05-36).
Author information
Authors and Affiliations
Corresponding author
Additional information
Publisher’s note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Rights and permissions
About this article
Cite this article
Sirawattanamongkol, T., Maswanna, T. & Maneeruttanarungroj, C. A newly isolated green alga Chlorella sp. KLSc59: potential for biohydrogen production. J Appl Phycol 32, 2927–2936 (2020). https://doi.org/10.1007/s10811-020-02140-1
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10811-020-02140-1