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The morphological, developmental and molecular landscape of Grateloupia qingdaoensis Li et Ding

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Abstract

The rhodophyte marine algal genus Grateloupia (Halymeniaceae, Halymeniales) is distributed worldwide, including in offshore southeastern China. Here, we investigated morphology, carpospore development, ecological habits, and molecular analyses of a rarely studied species, Grateloupia qingdaoensis. The carpogonial branch ampullae and auxiliary cell ampullae were the typical Grateloupia type (6cpb-5auxb type) and the type of spore development was “mediate discal type”. The life history included homotypic gametophytes (haploid), carposporophytes (diploid), and tetrasporophytes (diploid) and showed a typical isomorphic alternation of generations. By using single-factor and orthogonal experiments, we explored the effects of temperature, irradiance and seawater salinity on the early development of G. qingdaoensis. Each ecological factor showed distinct effect on the early development of G. qingdaoensis. Additionally, we found a significant interactive effect between temperature and irradiance on discord crust growth. The optimum conditions for the early growth of discoid crust and upright thalli were 20 °C, 80 μmol photons m−2 s−1 irradiance and 30‰ seawater salinity. To clarify its taxonomical status, we constructed the phylogenetic tree of rbcL gene sequences. Results showed that there was no pairwise divergence between G. qingdaoensis and G. asiatica from China. Our findings indicate that previous records without precise and comprehensive comparison and molecular analyses need to be re-examined to clarify the global distribution of this species.

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References

  • Bidart-Bouzat MG (2004) Herbivory modifies the lifetime fitness response of Arabidopsis thaliana to elevated CO2. Ecology 85:297–303

    Article  Google Scholar 

  • Cao C, Zhao F, Guo S, Wang H (2015) Effect of temperature and light on growth of Grateloupia tenuis. Oceanol Limnol 46:298–304

    Google Scholar 

  • Chen W, Song Z, Huang Z (2013) Effects of temperature and irradiance on early development of Grateloupia livida. South China Fish Sci 9:14–19

    CAS  Google Scholar 

  • Choi HG, Kim YS, Kim JH, Lee SJ, Park EJ, Ryu J, Nam KW (2006) Effects of temperature and salinity on the growth of Gracilaria verrucosa and G. chorda, with the potential for mariculture in Korea. J Appl Phycol 18:269–277

    Article  Google Scholar 

  • Cruces E, Flores-Molina MR, Díaz MJ, Huovinen P, Gómez I (2018) Phenolics as photoprotective mechanism against combined action of UV radiation and temperature in the red alga Gracilaria chilensis? J Appl Phycol 30:1247–1257

    Article  CAS  Google Scholar 

  • Deng Y, Tang X, Huang B, Ding L (2011) The temperature character of marine green alga, Chaetomorpha valida, with analysis of its diffusion potential in marine algal flora of China. Oceanol Limnol 42:404–408

    Google Scholar 

  • Elisa P, Zenilda LB, Luciane O, Débora T, Felipe B, Éder C, Carmen S (2016) Evaluation of salinity effects on the release, adhesion, and germination of the tetraspores of Gelidium floridanum (Rhodophyta, Florideophyceae). J Appl Phycol 28:2925–2938

    Article  Google Scholar 

  • Graham L, Wilcox L (2000) Algae. Prentice-Hall, Upper Saddle River, 640 p

    Google Scholar 

  • Guo H, Yao J, Sun Z, Duan D (2015) Effects of salinity and nutrients on the growth and chlorophyll fluorescence of Caulerpa lentillifera. Chin J Oceanol Limnol 33:410–418

    Article  CAS  Google Scholar 

  • Haag JJ, Coupe MD, Cahill JF (2010) Antagonistic interactions between competition and insect herbivory on plant growth. J Ecol 92:156–167

    Article  Google Scholar 

  • Huber H, Kane NC, Heschel MS, von Wettberg EJ, Banta J, Leuck AM, Schmitt J (2004) Frequency and microenvironmental pattern of selection on plastic shade-avoidance traits in a natural population of Impatiens capensis. Am Nat 163:548–563

    Article  Google Scholar 

  • Kawaguchi S, Wang H, Horiguchi T, Sartoni G, Masuda M (2001) A comparative study of the red alga Grateloupia filicina (Halymeniaceae) from the northwestern Pacific and Mediterranean with the description of Grateloupia asiatica sp. nov. J Phycol 37:433–442

    Article  CAS  Google Scholar 

  • Li F, Jiang P, Zhao S, Wang H (2016) Research on the revision of Grateloupia fastigiata Li et Ding (Rhodophyta, Halymeniaceae) based on morphological observations and rbcL sequence analyses. Acta Hydrobiol Sinica 40:1249–1256

    Google Scholar 

  • Liu F, Li W (1986) A comparative study un the mode of sporogensis of Grateloupia filicina C Ag and Grateloupia ramosissima Okam. J Fish China 10:281–287

    Google Scholar 

  • McLachlan J (2011) The effect of salinity on growth and chlorophyll content in representative classes of marine algae. Can J Microbiol 7:399–406

    Article  Google Scholar 

  • Pritchard DW, Hurd CL, Beardall J, Hepburn CD (2013) Survival in low light: photosynthesis and growth of a red alga in relation to measured in situ irradiance. J Phycol 49:867–879

    CAS  PubMed  Google Scholar 

  • ProProvasoli L, Watanabe H, Hattori A (1968) Media and prospects for the cultivation of marine algae. In: Watanabe A, Hattori A (eds) Culture and collection of algae US–Japan conference. Japanese Society of Plant Physiology, Hakone, pp 63–75

    Google Scholar 

  • Ryuta T, Shingo I, Gregory NN (2013) The effect of light and temperature on the growth and photosynthesis of Gracilariopsis chorda (Gracilariales, Rhodophtya) from geographically separated locations of Japan. J Appl Phycol 25:1863–1872

    Article  Google Scholar 

  • Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) Mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731–2739

    Article  CAS  Google Scholar 

  • Team (2012) R: a language and environment for statistical computing. Computing 1:12–21

    Google Scholar 

  • Tian Y, Liu Y, Wang H (2017) Early development and life cycle of Grateloupia lanceolata. Oceanol Limnol 48:113–121

    Google Scholar 

  • Wang H, Kawaguchi S, Horiguchi T, Masuda M (2000) Reinstatement of Grateloupia catenata (Rhodophyta, Halymeniaceae) on the basis of morphology and rbcL sequences. Phycologia 39:228–237

    Article  Google Scholar 

  • Wei Z, Zhu M, Yu G (2012) Occurrence of planktonic cyanobacterium Tychonema bouurrellyi in Erhai Lake and its taxonomic studies. Acta Hydrobiol Sinica 36:1171–1175

    Article  CAS  Google Scholar 

  • Xia B, Wang Y, Xia E, Li W, Ding Z (2004) Grateloupia C. Agardh. In: Xia B (ed) Flora Algarum Marinarum Sinicarum (Tomus II Rhodophyta, No.3 Gelidiales, Cryptonemiales, Hildenbrandiales). Science Press, Beijing, pp 127–130

    Google Scholar 

  • Yu CH, Lim PE, Phang SM (2013) Effects of irradiance and salinity on the growth of carpospore-derived tetrasporophytes of Gracilaria edulis and Gracilaria tenuistipitata var liui (Rhodophyta). J Appl Phycol 25:787–794

    Article  CAS  Google Scholar 

  • Zhang X (2013) A study on the early development and temperature and light intensity on the five species, (Gigartinales, Rhodophyta) from China. Liaoning Normal University, Dissertation

    Google Scholar 

  • Zou M, Zhang S, Zhou XJ (2017) The influence of temperature and light intensity on Sargassum vachellianum detritus decomposition. Chin J Ecol 36:428–435

    Google Scholar 

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Funding

This study was financially supported by the National Natural Science Foundation of China (No. 31570209).

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Author notes

  1. Jing Liu, Jingrui Li and Yao Bian contributed equally to this work.

Authors and Affiliations

  1. College of Life Sciences, Liaoning Normal University, Dalian, 116000, China

    Jing Liu, Jingrui Li, Yao Bian, Yuanyuan Ding, Huina Wang & Hongwei Wang

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  1. Jing Liu
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  2. Jingrui Li
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  3. Yao Bian
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  4. Yuanyuan Ding
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  5. Huina Wang
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  6. Hongwei Wang
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Correspondence to Hongwei Wang.

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Liu, J., Li, J., Bian, Y. et al. The morphological, developmental and molecular landscape of Grateloupia qingdaoensis Li et Ding. J Appl Phycol 32, 2093–2103 (2020). https://doi.org/10.1007/s10811-020-02046-y

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  • DOI: https://doi.org/10.1007/s10811-020-02046-y

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