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Macromolecule extracted from Gracilaria caudata reduces inflammation and restores hepatic function in nimesulide-induced hepatic damage

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Abstract

Seaweeds are a source of novel bioactive compounds such as sulfated polysaccharides (PLS) that are not found in plants, but that may confer health-promoting properties. Sulfated polysaccharides extracted from seaweeds have several biologically beneficial effects. The PLS extracted from the red alga Gracilaria caudata are known to have anti-inflammatory, antinociceptive, and gastroprotective activities. The present study aimed to evaluate the hepatoprotective effect of PLS obtained from G. caudata on a nimesulide-induced liver damage model. Hepatic lesions were induced in mice by oral doses of nimesulide (200 mg kg−1) administered once daily for 5 days. Once a day, 30 min after administration of nimesulide, PLS was administered intraperitoneally at concentrations of 2.5, 5, and 10 mg kg−1. Subsequently, blood was collected for biochemical tests and the liver was removed to evaluate inflammatory parameters. Administration of PLS at the dose of 10 mg kg−1 led to a significant reduction in hepatic injury, liver weight/animal weight ratio, the levels of a neutrophil migration marker (myeloperoxidase), pro-inflammatory cytokines (interleukin-1β and tumor necrosis factor-α), nitrate and nitrite, oxidative stress markers, and hepatic function markers. As per our results, the PLS extracted from G. caudata was able to modulate the inflammatory response in nimesulide-induced hepatic damage in addition to re-establishing the hepatic homeostatic functions.

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Funding

The authors received financial and structural support from the Brazilian Agency for Scientific and Technological Development-CNPq (Brazil) and Federal University of Piauí, UFPI (Brazil).

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Authors and Affiliations

  1. LAFFEX - Laboratory of Experimental Physiopharmacology, Biotechnology and Biodiversity Center Research (BIOTEC), Federal University of Piauí, Av. São Sebastião, no. 2819, Parnaíba, PI, CEP 64202-020, Brazil

    Genilson José Dias Júnior, Sarah Izabelly Alves Lemos, Tarcisio Vieira de Brito, Cynthia Maria Carvalho Pereira, José Simião da Cruz Júnior, Jayro dos Santos Ferreira, Lauanda da Rocha Rodrigues, José Victor do Nascimento Lima & André Luiz dos Reis Barbosa

  2. LEFFAG—Laboratory of Physiopharmacology Study of Gastrointestinal Tract, Federal University of Ceará, Fortaleza, CE, Brazil

    Carlos Eduardo da Silva Monteiro, Alvaro Xavier Franco & Pedro Marcos Gomes Soares

  3. Department of Biomedicine, LAPHIS - Laboratory of Analysis and Histological Processing, Federal University of Piauí, Parnaíba, PI, 64202-020, Brazil

    Even Herlany Pereira Alves & Daniel Fernando Pereira Vasconcelos

  4. Department of Biochemistry and Molecular Biology, Laboratory of Proteins and Carbohydrates of Marine Algae, Federal University of Ceará, Fortaleza, Brazil

    Francisco Clark Nogueira Barros & Ana Lúcia Ponte Freitas

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  1. Genilson José Dias Júnior
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  2. Sarah Izabelly Alves Lemos
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Correspondence to André Luiz dos Reis Barbosa.

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Júnior, G.J.D., Lemos, S.I.A., de Brito, T.V. et al. Macromolecule extracted from Gracilaria caudata reduces inflammation and restores hepatic function in nimesulide-induced hepatic damage. J Appl Phycol 32, 1511–1520 (2020). https://doi.org/10.1007/s10811-020-02039-x

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