Ultrasound-assisted turbine bead milling for disintegration of Nannochloropsis oculata cells

  • Xijun Liu
  • Zhidong Pan
  • Yanmin WangEmail author


The disintegration of Nannochloropsis oculata cells in a turbine-stirred bead mill with an ultrasonic system was investigated. The effect of ultrasonic assistance on the suspension viscosity and the percentage of cell disintegration of N. oculata in the disintegration process are discussed. The results show that ultrasonic assistance has a significant effect on the viscosity reduction of N. oculata suspension in the disintegration process, resulting in an increase in the disintegration efficiency. The effective disintegration can be obtained under optimum condition (i.e., ultrasonic power of 100 W, ultrasonic time interval of 5 s, and disintegration time of 30 min). In addition, the disintegration behaviors with and without ultrasonic assistance were also simulated by population balance modeling (PBM). Based on the simulated results, ultrasonic assistance during disintegration process can increase the disruption efficiency of N. oculata cells and the cells are mainly disrupted by compression and shear effects in the mill.


Turbine-stirred bead mill Ultrasound Nannochloropsis oculata Disintegration Population balance model 


Funding information

This work was supported by the Funding of Guangdong Science and Technology Department, China (No. 2015A020209019).

Supplementary material

10811_2018_1702_MOESM1_ESM.docx (50 kb)
ESM 1 (DOCX 50 kb)


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Copyright information

© Springer Nature B.V. 2018

Authors and Affiliations

  1. 1.School of Materials Science & EngineeringSouth China University of TechnologyGuangzhouChina

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