Ultrasound-assisted turbine bead milling for disintegration of Nannochloropsis oculata cells
The disintegration of Nannochloropsis oculata cells in a turbine-stirred bead mill with an ultrasonic system was investigated. The effect of ultrasonic assistance on the suspension viscosity and the percentage of cell disintegration of N. oculata in the disintegration process are discussed. The results show that ultrasonic assistance has a significant effect on the viscosity reduction of N. oculata suspension in the disintegration process, resulting in an increase in the disintegration efficiency. The effective disintegration can be obtained under optimum condition (i.e., ultrasonic power of 100 W, ultrasonic time interval of 5 s, and disintegration time of 30 min). In addition, the disintegration behaviors with and without ultrasonic assistance were also simulated by population balance modeling (PBM). Based on the simulated results, ultrasonic assistance during disintegration process can increase the disruption efficiency of N. oculata cells and the cells are mainly disrupted by compression and shear effects in the mill.
KeywordsTurbine-stirred bead mill Ultrasound Nannochloropsis oculata Disintegration Population balance model
This work was supported by the Funding of Guangdong Science and Technology Department, China (No. 2015A020209019).
- Schütte H, Kula MR (1991) Pilot- and process-scale techniques for cell disruption. Biotechnol Appl Biochem 12:599–620Google Scholar
- Schwede S, Kowalczyk A, Gerber M, Span R (2011) Influence of different cell disruption techniques on mono digestion of algal biomass. World Renewable Energy Congress - Bioenergy Technology (BE)-Sweden, pp. 41–7Google Scholar