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Photosynthetic activity of Prorocentrum donghaiense Lu acclimated to phosphorus limitation and its photosynthetic responses to nutrient depletion

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Abstract

Prorocentrum donghaiense blooms periodically occur in late spring and early summer in the phosphorus (P)-limited East China Sea; however, few studies have been conducted in nutrient conditions that resembled those in the East China Sea. To study the actual photosynthetic status of P. donghaiense in P-limited conditions, we established stable P-limited conditions using semi-continuous culture at two dilution rates (0.1 and 0.3 day−1). When the external nutrient concentrations remained steady after 26 days, time did not affect the maximum quantum yield (Fv/Fm), normalized Stern-Volmer quenching (NPQNSV), maximum light use efficiency (α), maximum relative electron transport rate (rETRmax), and light saturation intensity (Ik). Prorocentrum donghaiense acclimated to P limitation, and the high actual quantum yield of photosystem II (ΦPSII) and rETRmax, low NPQNSV at 0.3 day−1, and increased ΦPSII with time at 0.3 day−1 indicated the photosynthetic advantages of P. donghaiense at a high growth rate. To test the responses of P-limitation-acclimated P. donghaiense to nutrient inputs, nutrient-addition experiments were conducted. Nitrate and phosphate addition thoroughly depleted P or nitrogen (N). Greater decreases in Fv/Fm, ΦPSII, α and rETRmax, and higher NPQNSV derived from rapid light curves (RLCs) were found under the P-depletion condition than under the N depletion condition. These results showed that the P-limitation-acclimated P. donghaiense had a weaker photosynthetic tolerance to P depletion. Given the differences in the photosynthetic tolerance of P. donghaiense to nutrient depletion, our results suggested that reduced P inputs should be considered during the management of P. donghaiense blooms.

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Acknowledgements

We thank two anonymous reviewers for their helpful comments. We would like to express our utmost appreciation and respect to the late Prof Mingyuan Zhu in the First Institute of Oceanography, State Oceanic Administration (SOA) for his valuable suggestions on the semi-continuous algal culture. This work was supported by the National Natural Science Foundation of China (No. 41506191).

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  1. Ping Sun

    Present address: First Institute of Oceanography, Ministry of Natural Resources, 6 Xianxialing Road, 266061, Qingdao, People’s Republic of China

Authors and Affiliations

  1. First Institute of Oceanography, Ministry of Natural Resources, 6 Xianxialing Road, 266061, Qingdao, People’s Republic of China

    Kai-Ming Sun, Ming Xin, Yan Li, Ruixiang Li & Zongling Wang

  2. Laboratory of Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology, 266237, Qingdao, People’s Republic of China

    Kai-Ming Sun & Ping Sun

  3. College of Marine Life, Ocean University of China, 266003, Qingdao, People’s Republic of China

    Kai-Ming Sun & Xuexi Tang

  4. College of Environmental Science and Engineering, Ocean University of China, 266100, Qingdao, People’s Republic of China

    Ping Sun

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Sun, KM., Xin, M., Sun, P. et al. Photosynthetic activity of Prorocentrum donghaiense Lu acclimated to phosphorus limitation and its photosynthetic responses to nutrient depletion. J Appl Phycol 31, 1721–1732 (2019). https://doi.org/10.1007/s10811-018-1701-1

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