Journal of Applied Phycology

, Volume 31, Issue 1, pp 533–545 | Cite as

Evaluation of biomethane potential of waste algal biomass collected from eutrophied lake: effect of source of inocula, co-substrate, and VS loading

  • Pushpendar Kumar
  • Sanjeev Kumar PrajapatiEmail author
  • Anushree MalikEmail author
  • Virendra Kumar Vijay


Present study used sequential optimization of inoculum, co-substrate, and volatile solid (VS) loading rate to enhance biomethanation of waste algal biomass (HL) collected from eutrophied Hauz Khas lake, India. The mixed algal biomass was rich in lipids (45% TS) with a C/N ratio of 10.08 and 88% VS. Higher methane yield (279 mL g−1 VSfed) was observed using inoculum from algae-cattle dung based biogas plant compared to 170–200 mL g−1 VSfed using other inocula (from sewage sludge, Jatropha/ Karanja cake and cattle dung based plant). Biomethane yield was further enhanced (312 mL g−1 VSfed) by co-digestion with cattle dung (HL + CD) as compared to 282 mL g−1 VSfed for HL + WAS (waste activated sludge). Further, VS loading of 5 g VS L−1 gave highest methane yield of 315 mL g−1 VSfed, while a decrease (240 and 224 mL g−1 VSfed) was observed at 10 and 15 g VS L−1, respectively.


Eutrophication Waste algal biomass Co-digestion Biomethanation Bioenergy 


Funding information

The authors gratefully acknowledge the Department of Biotechnology (Grant no. DBT/IC-2/Indo-Brazil/2016-19/06), Govt. of India for financial support.


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Copyright information

© Springer Nature B.V. 2018

Authors and Affiliations

  1. 1.Applied Microbiology Lab (AML), Centre for Rural Development and TechnologyIndian Institute of Technology DelhiNew DelhiIndia
  2. 2.Biogas Laboratory, Centre for Rural Development and TechnologyIndian Institute of Technology DelhiNew DelhiIndia
  3. 3.BioResource Engineering Lab (BREL), Department of Chemical and Biochemical EngineeringIndian Institute of Technology (IIT) PatnaPatnaIndia

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