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CO2 fixation capability of Chlorella sp. and its use in treating agricultural wastewater

  • Harizah Bajunaid Hariz
  • Mohd Sobri Takriff
  • Muneer M. Ba-Abbad
  • Nazlina Haiza Mohd Yasin
  • Noor Irma Nazashida Mohd Hakim
8th Asian Pacific Phycological Forum

Abstract

Palm oil mill effluent (POME) is a highly polluted agro-industrial wastewater. The CO2 in industrial flue gas requires treatment before it can be discharged into the environment. Utilizing microalgae as the agent to treat wastewater and industrial flue gas is a waste-to-wealth approach. The resulting biomass can be commercialized in the form of valuable products. Chlorella sp. is a microalgal species that can tolerate the pollutant load and has been proven to be a suitable species for CO2 fixation. In this study, Chlorella sp. was cultivated in POME with the aim of reducing the pollutants in the POME and simultaneously capturing CO2. The optimization of the operational conditions of this microalgae-based treatment system was carried out using the response surface methodology (RSM) face centered-central composite design (FC-CCD). Operational factors include the air concentration of CO2 (10–25% v/v), the inlet gas flow rate of 500–2000 mL min−1, and initial inoculum concentration (10–30% v/v) of Chlorella sp. cultivated in POME. The target deliverables include the maximum amount of CO2 fixed by Chlorella sp. and the total nitrogen (TN) reduction as indicators of pollutant reduction by this treatment system. We found that a limited supply of CO2 caused growth limitation, while excess CO2 resulted in acid production that triggered microalgae growth inhibition. The optimum operational conditions were 10% v/v CO2, 1670 mL min−1 aeration rate, and 24.8% v/v inoculum concentration, predicted to simultaneously fix CO2 at 0.12 g of CO2 L−1 day−1 and reduce 80.9% TN, respectively.

Keywords

Chlorophyta Bio-fixation Effluent treatment Greenhouse gases (GHGs) Palm oil mill effluent (POME) Phycoremediation 

Notes

Acknowledgements

The authors gratefully acknowledge Yayasan Sime Darby for the scholarship.

Funding information

This study received financial support from UKM-YSD Research Grant and Dana Impak Perdana DIP-2017-007.

References

  1. Ahmad AL, Ismail S, Bhatia S (2003) Water recycling from palm oil mill effluent (POME) using membrane technology. Desalination 157:87–95CrossRefGoogle Scholar
  2. Ahmad AD, Salihon J, Tao DG (2015) Evaluation of CO2 sequestration by microalgae culture in palm oil mill effluent (POME) medium. Adv Mater Res 1113:311–316CrossRefGoogle Scholar
  3. Amin S (2009) Review on biofuel oil and gas production processes from microalgae. Energy Convers Manag 50:1834–1840CrossRefGoogle Scholar
  4. Barbosa MJ, Albrecht M, Wijffels RH (2003) Hydrodynamic stress and lethal events in sparged microalgae cultures. Biotechnol Bioeng 83:112–120CrossRefPubMedGoogle Scholar
  5. Bohutskyi P, Liu K, Nasr LK, Byers N, Rosenberg JN, Oyler GA, Bouwer EJ (2015) Bioprospecting of microalgae for integrated biomass production and phytoremediation of unsterilized wastewater and anaerobic digestion centrate. Appl Microbiol Biotechnol 99:6139–6154CrossRefPubMedGoogle Scholar
  6. Bohutskyi P, Kligerman DC, Byers N, Nasr LK, Cua C, Chow S, Bouwer EJ (2016) Effects of inoculum size, light intensity, and dose of anaerobic digestion centrate on growth and productivity of Chlorella and Scenedesmus microalgae and their poly-culture in primary and secondary wastewater. Algal Res 19:278–290CrossRefGoogle Scholar
  7. Borowitzka MA (1998) Limits to growth. In: Wong YS, Tam NFY (eds) Wastewater treatment with algae. Springer, Berlin, pp 203–226CrossRefGoogle Scholar
  8. Cheng J, Huang Y, Feng J, Sun J, Zhou J, Cen K (2013) Improving CO2 fixation efficiency by optimizing Chlorella PY-ZU1 culture conditions in sequential bioreactors. Bioresour Technol 144:321–327CrossRefPubMedGoogle Scholar
  9. Chisti Y (2007) Biodiesel from microalgae. Biotechnol Adv 25:294–306CrossRefPubMedGoogle Scholar
  10. Chiu YS, Kao CY, Chen CH, Kuan TC, Ong SC, Lin CS (2008) Reduction of CO2 by a high-density culture of Chlorella sp. in a semi-continuous photobioreactor. Bioresour Technol 99:3389–3396CrossRefPubMedGoogle Scholar
  11. Choi HJ, Lee SM (2012) Effects of microalgae on the removal of nutrients from wastewater: various concentrations of Chlorella vulgaris. Environ Eng Res 17:S3–S8CrossRefGoogle Scholar
  12. Contreras A, García F, Molina E, Merchuk JC (1998) Interaction between CO2-mass transfer, light availability, and hydrodynamic stress in the growth of Phaeodactylum tricornutum in a concentric tube airlift photobioreactor. Biotechnol Bioeng 60:317–325CrossRefPubMedGoogle Scholar
  13. Cuéllar-Franca RM, Azapagic A (2015) Carbon capture, storage and utilisation technologies: a critical analysis and comparison of their life cycle environmental impacts. J CO2 Util 9:82–102CrossRefGoogle Scholar
  14. Di Iaconi C, Ramadori R, Lopez A (2006) Combined biological and chemical degradation for treating a mature municipal landfill leachate. Biochem Eng J 31:118–124CrossRefGoogle Scholar
  15. Ding J, Zhao F, Cao Y, Xing L, Liu W, Mei S, Li S (2015) Cultivation of microalgae in dairy farm wastewater without sterilization. Int J Phytoremediation 17:222–227CrossRefPubMedGoogle Scholar
  16. Flynn KJ (1991) Algal carbon–nitrogen metabolism: a biochemical basis for modelling the interactions between nitrate and ammonium uptake. J Plankton Res 13:373–387CrossRefGoogle Scholar
  17. Gan PY, Li ZD (2014) Econometric study on Malaysia’s palm oil position in the world market to 2035. Renew Sust Energ Rev 39:740–747CrossRefGoogle Scholar
  18. Hammouda O, Gaber A, Abdelraouf N (1995) Microalgae and wastewater treatment. Ecotoxicol Environ Saf 31:205–210CrossRefPubMedGoogle Scholar
  19. Hanagata N, Takeuchi T, Fukuju Y, Barnes DJ, Karube I (1992) Tolerance of microalgae to high CO2 and high temperature. Phytochemistry 31:3345–3348CrossRefGoogle Scholar
  20. Hariz HB, Takriff MS (2017) Palm oil mill effluent treatment and CO2 sequestration by using microalgae-sustainable strategies for environmental protection. Environ Sci Pollut Res 24:20209–20240CrossRefGoogle Scholar
  21. He Q, Yang H, Hu C (2015) Optimizing light regimes on growth and lipid accumulation in Ankistrodesmus fusiformis H1 for biodiesel production. Bioresour Technol 198:876–883CrossRefPubMedGoogle Scholar
  22. Hsueh HT, Chu H, Yu ST (2007) A batch study on the bio-fixation of carbon dioxide in the absorbed solution from a chemical wet scrubber by hot spring and marine algae. Chemosphere 66:878–886CrossRefPubMedGoogle Scholar
  23. Hulatt CJ, Thomas DN (2011) Productivity, carbon dioxide uptake and net energy return of microalgal bubble column photobioreactors. Bioresour Technol 102:5775–5787CrossRefPubMedGoogle Scholar
  24. Janssen M, Tramper J, Mur LR, Wijffels RH (2003) Enclosed outdoor photobioreactors: light regime, photosynthetic efficiency, scale-up, and future prospects. Biotechnol Bioeng 81:193–210CrossRefPubMedGoogle Scholar
  25. Kastanek F, Sabata S, Solcova O, Maleterova Y, Kastanek P, Branyikova I, Kuthan K, Zachleder V (2010) In-field experimental verification of cultivation of microalgae Chlorella sp. using the flue gas from a cogeneration unit as a source of carbon dioxide. Waste Manag Res 28:961–966CrossRefPubMedGoogle Scholar
  26. Khalid AAH, Yaakob Z, Sheikh Abdullaha SR, Takriff MS (2016) Enhanced growth and nutrients removal efficiency of Characium sp. cultured in agricultural wastewater via acclimatized inoculum and effluent recycling. J Environ Chem Eng 4:3426–3432CrossRefGoogle Scholar
  27. Khalid AAH, Yaakob Z, Abdullah SRS, Takriff MS (2017) Growth improvement and metabolic profiling of native and commercial Chlorella sorokiniana strains acclimatized in recycled agricultural wastewater. Bioresour Technol 247:930–939CrossRefGoogle Scholar
  28. Korbhati BK, Aktas N, Tanyolac A (2007) Optimization of electrochemical treatment of industrial paint wastewater with response surface methodology. J Hazard Mater 148:83–90CrossRefGoogle Scholar
  29. Kumar K, Dasgupta CN, Nayak B, Lindblad P, Das D (2011) Development of suitable photobioreactors for CO2 sequestration addressing global warming using green algae and cyanobacteria. Bioresour Technol 102:4945–4953CrossRefPubMedGoogle Scholar
  30. Lau PS, Tam NFY, Wong YS (1995) Effect of algal density on nutrient removal from primary settled wastewater. Environ Pollut 89:59–66, 249CrossRefGoogle Scholar
  31. Lee RA, Lavoie JM (2013) From first-to third-generation biofuels: challenges of producing a commodity from a biomass of increasing complexity. Anim Front 3:6–11CrossRefGoogle Scholar
  32. Lee YK, Ding SY, Hoe CH, Low CS (1996) Mixotrophic growth of Chlorella sorokiniana in outdoor enclosed photobioreactor. J Appl Phycol 8:163–169CrossRefGoogle Scholar
  33. Li Y, Horsman M, Wu N, Lan CQ, Calero ND (2008) Biofuels from microalgae. Biotechnol Prog 24:815–820PubMedGoogle Scholar
  34. Lopes J, Eduardo LMCF, Franco TT (2008) Biomass production and carbon dioxide fixation by Aphanothece microscopica Nägeli in a bubble column photobioreactor. Biochem Eng J 40:27–34CrossRefGoogle Scholar
  35. Lu S, Wang J, Niu Y, Yang J, Zhou J, Yuan Y (2012) Metabolic profiling reveals growth related FAME productivity and quality of Chlorella sorokiniana with different inoculum sizes. Biotechnol Bioeng 109:1651–1662CrossRefPubMedGoogle Scholar
  36. Ma AN, Cheah SC, Chow MC, Yeoh B (1993) Current status of palm oil processing waste management. In: Yeoh BG, Chee KS, Phang SM, Isa Z, Idris A, Mohamed M (eds) Waste Management in Malaysia: current status and prospects for bioremediation, Ministry of Science, technology and Environment, Kuala Lumpur pp 111–136Google Scholar
  37. Madigan MT, Martinko JM, Parker J (2017) Brock biology of microorganisms, vol 13. PearsonGoogle Scholar
  38. Maeda K, Owada M, Kimura N, Omata K, Karub I (1995) CO2 fixation from flue gas on coal fired thermal power plant by microalgae. Energy Convers Manag 36:717–720CrossRefGoogle Scholar
  39. Marzbali MH, Mir AA, Pazoki M, Pourjamshidian R, Tabeshnia M (2017) Removal of direct yellow 12 from aqueous solution by adsorption onto Spirulina algae as a high-efficiency adsorbent. J Environ Chem Eng 5:1946–1956CrossRefGoogle Scholar
  40. Minillo A, Godoy HC, Fonseca GG (2013) Growth performance of microalgae exposed to CO2. J Clean Energy Technol 1:110–114CrossRefGoogle Scholar
  41. Moheimani NR (2013) Inorganic carbon and pH effect on growth and lipid productivity of Tetraselmis suecica and Chlorella sp (Chlorophyta) grown outdoors in bag photobioreactors. J Appl Phycol 25:387–398CrossRefGoogle Scholar
  42. Moheimani NR, Isdepsky A, Lisec J, Raes E, Borowitzka MA (2011) Coccolithophorid algae culture in closed photobioreactors. Biotechnol Bioeng 108:2078–2087CrossRefPubMedGoogle Scholar
  43. Mohsenpour FS, Willoughby N (2016) Effect of CO2 aeration on cultivation of microalgae in luminescent photobioreactors. Biomass Bioenergy 85:168–177CrossRefGoogle Scholar
  44. Mostert ES, Grobbelaar JU (1987) The influence of nitrogen and phosphorus on algal growth and quality in outdoor mass algal cultures. Biomass 13:219–233CrossRefGoogle Scholar
  45. Ogbonna JC, Yoshizawa H, Tanaka H (2000) Treatment of high strength organic wastewater by a mixed culture of photosynthetic microorganisms. J Appl Phycol 12:277–284CrossRefGoogle Scholar
  46. Park KH, Lee CG (2001) Effectiveness of flashing light for increasing photosynthetic efficiency of microalgal cultures over a critical cell density. Biotechnol Bioprocess Eng 6:189–193CrossRefGoogle Scholar
  47. Peng H, Wei D, Chen F, Chen G (2016) Regulation of carbon metabolic fluxes in response to CO2 supplementation in phototrophic Chlorella vulgaris: a cytomic and biochemical study. J Appl Phycol 28:737–745CrossRefGoogle Scholar
  48. Preisig HR, Andersen RA (2005) Historical review of algal culturing techniques. In: Andersen RA (ed) Algal culturing techniques. Academic, Amsterdam, pp 1–12Google Scholar
  49. Qin L, Wang Z, Sun Y, Shu Q, Feng P, Zhu L, Xu J, Yuan Z (2016) Microalgae consortia cultivation in dairy wastewater to improve the potential of nutrient removal and biodiesel feedstock production. Env Sci Pollut Res 23:8379–8387Google Scholar
  50. Rao CS, Sathish T, Brahamaiah P, Kumar TP, Prakasham RS (2009) Development of a mathematical model for Bacillus circulans growth and alkaline protease production kinetics. J Chem Technol Biotechnol 84:302–307CrossRefGoogle Scholar
  51. Rosenberg JN, Mathias A, Korth K, Betenbaugh MJ, Oyler GA (2011) Microalgal biomass production and carbon dioxide sequestration from an integrated ethanol biorefinery in Iowa: a technical appraisal and economic feasibility evaluation. Biomass Bioenergy 35:3865–3876Google Scholar
  52. Shanshan M, Yuanhui Z, Baoming L, Taili D, Do Z (2016) Nitrogen and biomass recovery from low carbon to nitrogen ratio wastewater by combing air stripping and microalgae cultivation. J Residuals Sci Tech 13:S23–S31CrossRefGoogle Scholar
  53. Singh BP (ed) (2013) Biofuel crops: production, physiology and genetics. CABI, BostonGoogle Scholar
  54. Sung MG, Shin WS, Kim W, Kwon JH, Yang JW (2014) Effect of shear stress on the growth of continuous culture of Synechocystis PCC 6803 in a flat-panel photobioreactor. Korean J Chem Eng 31:1233–1236CrossRefGoogle Scholar
  55. Suryata I, Svavarsson HG, Einarsso S, Brynjólfsdóttir Á, Maliga G (2010) Geothermal CO2 bio-mitigation techniques by utilizing microalgae at the Blue Lagoon, Iceland. Proceedings, 34th Workshop on Geothermal Reservoir Engineering. Stanford University, StanfordGoogle Scholar
  56. Thomas DM, Mechery J, Paulose SV (2016) Carbon dioxide capture strategies from flue gas using microalgae: a review. Environ Sci Pollut Res 23:16926–16940Google Scholar
  57. Tuantet K (2015) Microalgae cultivation for nutrient recovery from human urine. PhD Thesis, Wageningen UniversityGoogle Scholar
  58. Tuantet K, Janssen M, Temmink H, Zeeman G, Wijffels RH, Buisman CJN (2013) Microalgae growth on concentrated human urine. J Appl Phycol 26:287–297CrossRefGoogle Scholar
  59. Tuantet K, Temmink H, Zeeman G, Janssen M, Wijffels RH, Buisman CJN (2014) Nutrient removal and microalgal biomass production on urine in a short light-path photobioreactor. Water Res 55:162–174CrossRefPubMedGoogle Scholar
  60. Van Den Hende S, Vervaeren H, Boon N (2012) Flue gas compounds and microalgae: (bio-) chemical interactions leading to biotechnological opportunities. Biotechnol Adv 30:1405–1424CrossRefGoogle Scholar
  61. Varshney P, Sohoni S, Wangikar PP, Beardall J (2016) Effect of high CO2 concentrations on the growth and macromolecular composition of a heat- and high-light-tolerant microalga. J Appl Phycol 28:2631–2640CrossRefGoogle Scholar
  62. Vymazal J (1995) Algae and element cycling in wetlands. Lewis Publishers Inc.Google Scholar
  63. Watanabe K, Fujii K (2016) Isolation of high level CO2 preferring Picochlorum sp. strains and their biotechnological potential. Algal Res 18:135–143CrossRefGoogle Scholar
  64. Yahya L, Chik MN, Boosroh MH (2012) Effect of flue gas flow rate on growth and carbon fixation ability of Isochrysis sp., 2nd International Conference on Environment Science and BiotechnologyGoogle Scholar
  65. Yahya L, Chik MN, Harun I, Boosroh MH (2015) Optimising Isochrysis sp, carbon fixation: a step towards the greening of fossil fuel. WIT Trans Ecol Environ 206:183–192Google Scholar
  66. Yen H-W, Ho S-H, Chen C-Y, Chang J-S (2015) CO2, NOx and SOx removal from flue gas via microalgae cultivation: a critical review. Biotechnol J 10:829–839CrossRefPubMedGoogle Scholar

Copyright information

© Springer Science+Business Media B.V., part of Springer Nature 2018

Authors and Affiliations

  1. 1.Research Center for Sustainable Process Technology (CESPRO)Universiti Kebangsaan Malaysia (UKM)BangiMalaysia
  2. 2.Department of Chemical Engineering, Faculty of Engineering and PetroleumHadhramout University of Science & TechnologyMukallaYemen
  3. 3.School of Bioscience and Biotechnology, Faculty of Science and TechnologyUniversiti Kebangsaan Malaysia (UKM)BangiMalaysia
  4. 4.Sime Darby Research Sdn Bhd, R&D Centre – Carey IslandCarey IslandMalaysia

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