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The red microalga Flintiella sanguinaria as a new exopolysaccharide producer

  • 6th Congress of the International Society for Applied Phycology
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Abstract

Flintiella sanguinaria is a red unicellular microalgae belonging to Porphyridiophyceae with a high potential as an exopolysaccharide producer. A new culture medium was developed by a stoichiometry approach based on elemental analysis of microalgae, and culture conditions were improved after measurements of photosynthetic activity as a function of irradiance, temperature and pH. These optimal conditions were thus validated in a photobioreactor for the production of biomass and exopolysaccharide (EPS). After extraction and purification of the EPS, it was characterised by HPAEC-PAD, HPLC and FT-IR as a methylated and acetylated galactoxylan including a significant amount of rhamnose and glucuronic acid in its structure. Macromolecular conformation in dilute solution of native, deproteinised and desubstituted EPS from F. sanguinaria revealed that this polysaccharide had a strong associative behaviour in which hydrophobic interactions or hydrogen bonding but also proteins were implicated.

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Funding

This work was supported by the ANR POLYSALGUE project, grant ANR-15-CE21-0013 of the French Agence Nationale de la Recherche.

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Authors and Affiliations

  1. Université Clermont Auvergne, CNRS, Institut Pascal, 63000, Clermont-Ferrand, France

    Clément Gaignard, Vanessa Macao, Christine Gardarin, Philippe Michaud & Céline Laroche

  2. UNIROUEN, INSA Rouen, CNRS, PBS, Normandie Univ, 76000, Rouen, France

    Christophe Rihouey & Luc Picton

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  1. Clément Gaignard
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  2. Vanessa Macao
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  3. Christine Gardarin
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  4. Christophe Rihouey
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  6. Philippe Michaud
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  7. Céline Laroche
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Correspondence to Céline Laroche.

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Gaignard, C., Macao, V., Gardarin, C. et al. The red microalga Flintiella sanguinaria as a new exopolysaccharide producer. J Appl Phycol 30, 2803–2814 (2018). https://doi.org/10.1007/s10811-018-1389-2

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  • DOI: https://doi.org/10.1007/s10811-018-1389-2

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