Abstract
We isolated a new subarctic strain Tetradesmus obliquus IPPAS S-2023 (Scenedesmaceae, Chlorophyceae) from rock baths in the White Sea. To verify its taxonomic assignment, internal transcribed spacer 2 (ITS2) of the strain was sequenced and its secondary structure was compared with predicted ITS2 secondary structures of Scenedesmaceae. The analysis of the ITS2 made it possible to assign the new strain IPPAS S-2023 to the species T. obliquus. The ultrastructural studies and energy-dispersive X-ray spectroscopy (EDX) analysis revealed a marked accumulation of vacuolar inclusions enriched in phosphorus and nitrogen (N) as well as cytoplasmiс oil bodies. Most of predicted properties of biodiesel derived from the fatty acids profile of the strain grown in the N-free medium complied with the requirements of European and American standards. The results suggest that the new subarctic strain T. obliquus IPPAS S-2023 is a promising candidate for nutrients biosequestration and for biodiesel production. In a companion paper, we assess its biomass production capability and suitability and demonstrated suitability of IPPAS S-2023 as a reference strain for studies on elevated CO2 stress effects selection of carbon dioxide-tolerant microalgae by comparison with a CO2-tolerant strain IPPAS S-2014.
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Acknowledgments
Financial support of Russian Ministry of Science and Education (grant 14.616.21.0080) is gratefully acknowledged. The ultrastructure studies were carried out at the User Facilities Center of M.V. Lomonosov Moscow State University. The authors are grateful to Dr. Olga A. Kudryavtseva for isolation of the T. obliquus strain IPPAS S-2023 from rock baths. Assistance of Dr. Roman A. Sidorov with fatty acid analysis is greatly appreciated.
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Fig. S1
Secondary structure of the internal transcribed spacer 2 (ITS2) of the nuclear ribosomal gene cluster of the strain IPPAS-2023 predicted based on KX530785.1 sequence. The helixes are designated as I, II, III and IV. The substitutions between KX530785.1 versus its closest homologs found by BLAST and their position in the KX530785.1/ITS2-fragment are shown in the figure. (PDF 30 kb)
Fig. S2
Distribution of isolated and identified representatives of Tetradesmus obluquus by the countries (PNG 1627 kb)
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Ismagulova, T., Chekanov, K., Gorelova, O. et al. A new subarctic strain of Tetradesmus obliquus—part I: identification and fatty acid profiling. J Appl Phycol 30, 2737–2750 (2018). https://doi.org/10.1007/s10811-017-1313-1
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DOI: https://doi.org/10.1007/s10811-017-1313-1