Journal of Applied Phycology

, Volume 29, Issue 5, pp 2287–2295 | Cite as

Morphological and physiological differences among cultivation lines of Undaria pinnatifida in a common garden experiment using a tank culture system

  • Yoichi SatoEmail author
  • Tomonari Hirano
  • Hiroyuki Ichida
  • Motoko Murakami
  • Nobuhisa Fukunishi
  • Tomoko Abe
  • Shigeyuki Kawano


Undaria pinnatifida is grown for food and industrial materials worldwide; therefore, advanced breeding is needed to meet quality and productivity requirements. In this study, we examined regional lines of U. pinnatifida from five cultivation sites in Japan with different environmental conditions: Oga (OGA, the northern Sea of Japan coast), Hirota Bay (HRT, the northeastern Pacific coast), Matsushima Bay (MAT, the northeastern Pacific coast), Naruto (the Seto Inland Sea coast) and Shimonoseki (SIM, the southern Sea of Japan coast). The sporophytes of these lines were cultured in a tank culture system under controlled environmental conditions, and their morphological characteristics, nutrient uptake kinetics (V max, K s and V max/K s ), and carbon, nitrogen and phosphorus contents were determined. Sporophytes from MAT grew faster, whereas those from SIM were smaller than those from the other sites. Although the blade thickness of sporophytes cultivated in the sea significantly differs among cultivation sites in the previous study, there was no significant difference in blade thickness among the regional lines cultivated in the tank. Sporophytes from OGA had the greatest V max/K s values and significantly greater nitrogen contents than the other lines. Therefore, the morphological characteristics of MAT and SIM sporophytes, and the nutrient uptake kinetics of OGA sporophytes may have a genetic origin. This indicates that these lines may represent useful resources for selective breeding, with MAT sporophytes providing faster growth and OGA sporophytes being well-adapted to low-nutrient conditions.


Genetic differentiation Morphological characteristics Nutrient uptake Tank cultivation Phaeophyceae Undaria pinnatifida 



We sincerely thank Haruo Hosoya (Riken Vitamine Co., Ltd.), and Hironobu Watanabe, Katsunori Ono and Junichi Sato (Riken Food Co., Ltd.) for their guidance throughout this research. We are very grateful to Osamu Sakuma, Tatsuya Musashi and Takahiro Saido (Iwate Prefectural Fisheries Technology Centre), Masaki Yamaguchi (Coastal Regional Development Bureau, Department of Fisheries in Iwate Prefecture), Susumu Ohwada and Osamu Hatakeyama (Hirota Bay Fisheries Cooperative), Hiroshi Akama (Seafoods Akama Co., Ltd.), Akihito Tojyo (Tojyo Shouten), Kenji Yoshitake (Yamaguchi Prefecture Fisheries Cooperative), Nobuyuki Hosoda (Haedomari Fisheries Cooperative), Kazuyoshi Saito and Dr. Nobuyasu Nakabayashi (Akita Fisheries Centre), and Wataru Suzuki (Wataru Suisan) for providing the sporophytes of cultivated Undaria pinnatifida and cultivated Undaria, and for their generous support during the field surveys. We also thank Dr. Nobuyasu Nanba and Misaki Shinozuka (Kitasato University) and Dr. Kinuko Ito (Tohoku University) for their valuable advice and cooperation in the measurement of nutrients concentration, and carbon and nitrogen contents. We thank Yutaka Ito, Miho Mogamiya and Naoko Sasaki (Riken Food Co., Ltd.) for their technical support in measuring the morphological characteristics and cultivation in the CFCS. This study was partly supported by the Formation of Tohoku Marine Science Centre Project (Technical Development That Leads to the Creation of New Industries) from the Ministry of Education, Culture, Sports, Science, and Technology of Japan.

Supplementary material

10811_2017_1170_MOESM1_ESM.pptx (142 kb)
Supplemental Fig. 1 Typical Undaria pinnatifida individuals cultivated at each site for 140–150 days according to the methods described in Sato et al. (2016). HRT: Hirota Bay, MAT: Matsushima Bay, NAR: Naruto, OGA: Oga, SIM: Shimonoseki (PPTX 141 kb)
10811_2017_1170_MOESM2_ESM.xlsx (38 kb)
Supplemental Table 1 (XLSX 37 kb)
10811_2017_1170_MOESM3_ESM.xlsx (38 kb)
Supplemental Table 2 (XLSX 38 kb)
10811_2017_1170_MOESM4_ESM.xlsx (35 kb)
Supplemental Table 3 (XLSX 34 kb)
10811_2017_1170_MOESM5_ESM.xlsx (35 kb)
Supplemental Table 4 (XLSX 35 kb)
10811_2017_1170_MOESM6_ESM.xlsx (37 kb)
Supplemental Table 5 (XLSX 36 kb)


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Copyright information

© Springer Science+Business Media Dordrecht 2017

Authors and Affiliations

  1. 1.Riken Food Co., LtdTagajyoJapan
  2. 2.Nishina Center for Accelerator-Based ScienceRIKENSaitamaJapan
  3. 3.Faculty of AgricultureUniversity of MiyazakiMiyazakiJapan
  4. 4.Future Center InitiativeThe University of TokyoKashiwaJapan

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