The effect of environmental factors on fatty acid composition of Chromera velia (Chromeridae)
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The effect of light intensity, light spectral quality, temperature and salt concentration on the fatty acid composition of Chromera velia was studied. Chromera velia is a unicellular, marine, photosynthetic, eukaryotic alga and a close relative of the apicomplexan parasites. Chromera velia was able to grow at light intensities between 20 and 450 μmol photons m−2 s−1, in the temperature range 17–32 °C and at salinities between 0.2 and 1 M NaCl. The cells responded to variations in the growth regime by modifying fatty acid composition: the ratio of fully saturated palmitic acid (C16:0) and five times unsaturated eicosapentaenoic acid (C20:5n–3) was especially prone to variation. Intermediate fatty acids, namely stearic, linoleic and dihomo-γ-linolenic acids, changed minimally and were probably not involved in the response to the growth regimes. The highest proportion of eicosapentaenoic acid was observed when the cultures were maintained at 32 °C, at an irradiance of 80 μmol photons m−2 s−1, provided by an incandescent light source, under a 12-/12-h day/night photoperiod.
KeywordsChromera velia Chromeridae Fatty acids Eicosapentaenoic acid Light Temperature Salinity
Financial support was provided by the project 14-15728S of the Grant Agency of the Czech Republic and by the project “Algatech plus” LO1416 of the Ministry of Education of the Czech Republic. We wish to thank Anna Yeates for correcting the English in this manuscript.
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