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Journal of Applied Phycology

, Volume 29, Issue 4, pp 1791–1799 | Cite as

The effect of environmental factors on fatty acid composition of Chromera velia (Chromeridae)

  • Martin LukešEmail author
  • Mario Giordano
  • Ondřej Prášil
Article

Abstract

The effect of light intensity, light spectral quality, temperature and salt concentration on the fatty acid composition of Chromera velia was studied. Chromera velia is a unicellular, marine, photosynthetic, eukaryotic alga and a close relative of the apicomplexan parasites. Chromera velia was able to grow at light intensities between 20 and 450 μmol photons m−2 s−1, in the temperature range 17–32 °C and at salinities between 0.2 and 1 M NaCl. The cells responded to variations in the growth regime by modifying fatty acid composition: the ratio of fully saturated palmitic acid (C16:0) and five times unsaturated eicosapentaenoic acid (C20:5n–3) was especially prone to variation. Intermediate fatty acids, namely stearic, linoleic and dihomo-γ-linolenic acids, changed minimally and were probably not involved in the response to the growth regimes. The highest proportion of eicosapentaenoic acid was observed when the cultures were maintained at 32 °C, at an irradiance of 80 μmol photons m−2 s−1, provided by an incandescent light source, under a 12-/12-h day/night photoperiod.

Keywords

Chromera velia Chromeridae Fatty acids Eicosapentaenoic acid Light Temperature Salinity 

Notes

Acknowledgements

Financial support was provided by the project 14-15728S of the Grant Agency of the Czech Republic and by the project “Algatech plus” LO1416 of the Ministry of Education of the Czech Republic. We wish to thank Anna Yeates for correcting the English in this manuscript.

Supplementary material

10811_2017_1114_MOESM1_ESM.pdf (123 kb)
Fig. S1 (PDF 123 kb)
10811_2017_1114_MOESM2_ESM.pdf (69 kb)
Fig. S2 (PDF 68 kb)
10811_2017_1114_MOESM3_ESM.pdf (64 kb)
ESM. 3 (PDF 64 kb)

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Copyright information

© Springer Science+Business Media Dordrecht 2017

Authors and Affiliations

  1. 1.Institute of Microbiology ASCR, Centrum Algatech, Laboratory of PhotosynthesisOpatovický mlýnTřeboňCzech Republic
  2. 2.Faculty of SciencesUniversity of South BohemiaČeské BudějoviceCzech Republic
  3. 3.Laboratory of Algal and Plant Physiology, Dipartimento di Scienze della Vita e dell’AmbienteUniversità Politecnica delle MarcheAnconaItaly
  4. 4.National Research CouncilIstituto di Biologia Agro-Ambeintale e Forestale; Consiglio Nazionale delle Ricerche (IBAF-CNR)TerniItaly

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