Abstract
Ultrasound-assisted aqueous extraction (USAE) of crude fucoidan from Sargassum muticum using an ultrasonic bath (150 W and 40 kHz ) at room temperature (25 °C) for 5–30 min was studied. Fucoidan-containing extracts were separated by centrifugation and characterized by HPLC, HPSEC, FT-IR, and MALDI-TOF spectrometry. Crude fucoidan on the best conditions was composed by oligosaccharides of fucose:galactose:glucose:xylose:mannose:rhamnose in a mass ratio of 1:0.70:0.33:0.27:0.22:0.10. The maximum sulfate content was 39.54 ± 0.001-mg sulfate ion g−1 extract. Furthermore, extract contained 25-mg phlorotannins g−1 extract and showed an ABTS radical scavenging activity equivalent to 40-mg Trolox g−1 extract measured with the Trolox Equivalent Antioxidant Capacity (TEAC) assay. The cell viability of selected tumoral human cell lines was reduced up to 30% in the presence of crude extracts at 500 mg L−1.
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Acknowledgments
The authors are grateful to the Ministry of Science and Innovation of Spain for the support of the research project CTM2015-68503-R and Xunta de Galicia for the Inbiomed project. Both projects were partially funded by the FEDER Program of the European Union (“Unha maneira de facer Europa”). N.F.F. thanks the Spanish Ministry of Economy and Competitiveness for her FPI grant (BES-2013-064701).
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Flórez-Fernández, N., López-García, M., González-Muñoz, M.J. et al. Ultrasound-assisted extraction of fucoidan from Sargassum muticum . J Appl Phycol 29, 1553–1561 (2017). https://doi.org/10.1007/s10811-016-1043-9
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DOI: https://doi.org/10.1007/s10811-016-1043-9