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Anti-inflammatory action of the ethanolic extract from Sargassum serratifolium on lipopolysaccharide-stimulated mouse peritoneal macrophages and identification of active components

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Abstract

Sargassum, a genus of brown algae (Phaeophyceae) in the Sargassaceae family, comprises approximately 400 species in the world. Among them, Sargassum serratifolium has been reported to have high level of meroterpenoids, which have antioxidant, anti-inflammatory, and neuroprotective activities. This study investigated the anti-inflammatory mechanism of ethanolic extract of S. serratifolium (ESS) in lipopolysaccharide (LPS)-stimulated peritoneal macrophages and identified anti-inflammatory compounds in ESS. ESS inhibited LPS-induced nitric oxide (NO) and prostaglandin E2 and the expression of inducible nitric oxide synthase and cyclooxygenase-2. ESS also reduced the release of pro-inflammatory cytokines in a dose-dependent manner. LPS-induced nuclear factor-κB transcriptional activity and translocation into the nucleus were significantly inhibited by ESS treatment through the prevention of the degradation of inhibitor κB-α. Furthermore, ESS inhibited the production of pro-inflammatory cytokines in mouse serum. The main anti-inflammatory components in ESS were identified as sargahydroquinoic acid, sargachromenol, and sargaquinoic acid based on the inhibition of NO production. Our results indicate that ESS can be used as a potential source of therapeutic agents for the treatment of inflammatory diseases.

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Acknowledgments

This research was supported by the project “Developemnt of nutraceuticals from Sargassum serratifolium” funded by the Ministry of Oceans and Fisheries, Republic of Korea.

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Correspondence to Hyeung-Rak Kim.

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Joung, EJ., Gwon, WG., Shin, T. et al. Anti-inflammatory action of the ethanolic extract from Sargassum serratifolium on lipopolysaccharide-stimulated mouse peritoneal macrophages and identification of active components. J Appl Phycol 29, 563–573 (2017). https://doi.org/10.1007/s10811-016-0954-9

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  • DOI: https://doi.org/10.1007/s10811-016-0954-9

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