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Diets supplemented with seaweed affect metabolic rate, innate immune, and antioxidant responses, but not individual growth rate in European seabass (Dicentrarchus labrax)

Journal of Applied Phycology volume 28, pages 2061–2071 (2016)Cite this article

Abstract

This study investigated the effects of seaweed dietary supplementation on measures of fish performance including aerobic metabolism, digestive enzymes activity, innate immune status, oxidative damage, and growth rate using European seabass (Dicentrarchus labrax). Fish were fed for 49 days with three different diets: a control diet (CTRL), a Gracilaria-supplemented diet (GR7.5), and a mixed diet (Mix) composed of Gracilaria, Fucus, and Ulva genera representatives. All diets were isoenergetic (22 kJ g−1 adjusted for dry matter (DM)), isoproteic (47 %DM), and isolipidic (18 %DM) and tested in triplicate groups of 20 fish (initial body weight 25.5 ± 4.1 g). Final results showed similar growth rates and digestive activities between diets. Maximum and standard metabolic rates and aerobic metabolic scope revealed comparable results for the three diets. In contrast, fish fed with GR7.5 exhibited elevated routine metabolic rate (190.7 mg O2 kg−1 h−1). Fish fed with the GR7.5 and Mix diets had lower alternative complement pathway (ACH50) (62.5 and 63 units mL−1 respectively) than CTRL (84 units mL−1) GR7.5 increased lipid peroxidation and cholinesterase levels, as well as glutathione s-transferase activity. Mix diet increased glutathione reductase activity when compared to CTRL. Collectively, our findings suggest that dietary seaweed supplementation may alter seabass metabolic rate, innate immune, and antioxidant responses without compromising growth parameters.

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Acknowledgments

This study was carried out under the project SEABIOPLAS (grant agreement n° 606032), funded by the European Union Seventh Framework Programme (FP7/2007-2013), as well as partially supported by the Strategic Funding UID/Multi/04423/2013 through national funds provided by Foundation for Science and Technology (FCT) and European Regional Development Fund (ERDF), in the framework of the program PT2020. The research was also supported by a grant (SFRH/BPD/89473/2012) from the Foundation for Science and Technology (FCT) in Portugal to Jon C. Svendsen. We thank the anonymous reviewers for their constructive and helpful comments on the earlier versions of the manuscript.

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Authors and Affiliations

  1. CIIMAR – Centro Interdisciplinar de Investigação Marinha e Ambiental, Universidade do Porto, Rua dos Bragas 289, 4050-123, Porto, Portugal

    Maria J. Peixoto, Jon C. Svendsen, Luis F. Pereira, José F. M. Gonçalves & Rodrigo O. A. Ozório

  2. ICBAS – Instituto de Ciências Biomédicas de Abel Salazar, Universidade do Porto, Rua de Jorge Viterbo Ferreira 228, 4050-313, Porto, Portugal

    Maria J. Peixoto, Pedro Carvalho, José F. M. Gonçalves & Rodrigo O. A. Ozório

  3. National Institute of Aquatic Resources, Section for Freshwater Fisheries and Ecology, Technical University of Denmark, Vejlsøvej 39, DK-8600, Silkeborg, Denmark

    Jon C. Svendsen

  4. National Institute of Aquatic Resources, Section for Ecosystem Based Marine Management, Technical University of Denmark, Charlottenlund Slot, Jægersborg Alle 1, DK-2920, Charlottenlund, Denmark

    Jon C. Svendsen

  5. Department of Bioscience, Zoophysiology, Aarhus University, C. F. Møllers Allé 3, DK-8000, Aarhus C, Denmark

    Hans Malte

  6. ALGAPlus, Lda, Travessa Alexandre da Conceição S/N., 3830-196, Ílhavo, Portugal

    Rui Pereira

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  1. Maria J. Peixoto
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Peixoto, M.J., Svendsen, J.C., Malte, H. et al. Diets supplemented with seaweed affect metabolic rate, innate immune, and antioxidant responses, but not individual growth rate in European seabass (Dicentrarchus labrax). J Appl Phycol 28, 2061–2071 (2016). https://doi.org/10.1007/s10811-015-0736-9

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Keywords

  • Digestive enzymes
  • Growth rate
  • Innate immune response
  • Metabolic rate
  • Oxidative stress
  • Seaweeds