Changes in phytochemical content and pharmacological activities of three Chlorella strains grown in different nitrogen conditions
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The phytochemical content and biological activity of three Chlorella strains cultured in low (35 mg L−1) or high (700 mg L−1) nitrogen (N) and harvested on days 5 and 10 were evaluated. High N resulted in a higher biomass in Chlorella MACC 438 and MACC 452 while MACC 555 produced a higher biomass in low N. MACC 555 (low N/day 5) had the highest phenolic content, and MACC 438 in low N/day 5 and high N/day 5 accumulated the highest flavonoids and condensed tannins, respectively. Iridoids were most abundant in MACC 452 on low N/day 10. Harvest time had the greatest effect on the phytochemical content with phenolics, flavonoids, and condensed tannins decreasing over time and iridoids increasing in low N and decreasing in high N conditions. Extracts were more active in β-carotene-linoleic acid model compared to 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging assay. Most extracts had good antimicrobial activity. Extracts became less potent over time in the antioxidant, acetylcholinesterase inhibitory (AChE), and antimicrobial assays when growing in low N and more potent in the antioxidant and AChE assays when grown in high N. Thus, phytochemical content and biological activities of the three Chlorella strains were affected by N levels, harvest time, and strain.
KeywordsAcetylcholinesterase Antimicrobial Antioxidant Chlorophyta Microalgae Natural products
We appreciate the financial support from the Claude Leon Foundation, University of KwaZulu-Natal and National Research Foundation, South Africa. This work was also supported by the New Széchényi Plan, TÁMOP-4.2.2.A-11/1/KONV-2012-0003 Microalgal biotechnology in sustainable agriculture.
JVS and VÖ conceived and coordinated the project. WAS, AOA, and VÖ designed the experiment. ZM and VÖ were involved with the cultivation of the Chlorella strains. AOA and NAM conducted the photochemical and biological assays. AOA and WAS drafted the manuscript and all the authors read and approved the final version.
- Abedin RMA, Taha HM (2008) Antibacterial and antifungal activity of cyanobacteria and green microalgae. Evaluation of medium components by Plackett-Burman design for antimicrobial activity of Spirulina platensis. Glob J Biotech Biochem 3:22–31Google Scholar
- Amaro HM, Guedes CA, Malcata FX (2011) Antimicrobial activites of microalgae: an invited review. In: Méndez-Vilas A (ed) Science against microbial pathogens: communicating current research and technological advances. Formatex Research Center, Badajoz, Spain, pp 1272–1280Google Scholar
- Coesel SN, Baumgartner AC, Teles LM, Ramos AA, Henriques NM, Cancela L, Varela JCS (2008) Nutrient limitation is the main regulatory factor for carotenoid accumulation and for Psy and Pds steady state transcript levels in Dunaliella salina (Chlorophyta) exposed to high light and salt stress. Mar Biotechnol 10:602–611CrossRefPubMedGoogle Scholar
- Custódio L, Soares F, Pereira H, Barreira L, Vizetto-Duarte C, Rodrigues MJ, Rauter AP, Alberício F, Varela J (2014a) Fatty acid composition and biological activities of Isochrysis galbana T-ISO, Tetraselmis sp. and Scenedesmus sp.: possible application in the pharmaceutical and functional food industries. J Appl Phycol 26:151–161CrossRefGoogle Scholar
- Custódio L, Soares F, Pereira H, Rodrigues MJ, Barreira L, Rauter AP, Alberício F, Varela J (2014b) Botryococcus braunii and Nannochloropsis oculata extracts inhibit cholinesterases and protect human dopaminergic SH-SY5Y cells from H2O2-induced cytotoxicity. JAppl Phycol. doi: 10.1007/s10811-014-0369-4 Google Scholar
- Franco D, Sineiro J, Rubilar M, Sánchez M, Jerez M, Pinelo M, Costoya N, Núñez MJ (2008) Polyphenols from plant materials: extraction and antioxidant power. Electr J Env Agric Food Chem 7:3210–3216Google Scholar
- Nair BB, Krishika A (2011) Antibacterial activity of freshwater microalga (Scenedesmus sp.) against three bacterial strains. J Biosci Res 2:160–165Google Scholar
- Ndhlala AR, Aremu AO, Moyo M, Amoo SO, Van Staden J (2012) Acetylcholineterase inhibitors from plant sources: friends or foes? In: White CJ, Tait JE (eds) Cholinesterase: production, uses and health effects. Nova, New York, pp 67–98Google Scholar
- Ördög V (1982) Apparatus for laboratory algal bioassay. Int Rev Ges Hydrobiol 67:127–137Google Scholar