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Mitochondrial phylogenomics reveals a close relationship between Petalonia fascia (Scytosiphonaceae, Phaeophyceae) and Ectocarpus siliculosus

Journal of Applied Phycology volume 27, pages 1021–1028 (2015)Cite this article

Abstract

The complete mitochondrial (mt) genome sequence of the brown alga, Petalonia fascia (O.F. Müller) Kuntze was determined and analyzed in this study. The 38,053-bp mt genome contains 68 genes, including 3 ribosomal RNA genes, 25 transfer RNA genes, 35 protein-coding genes, and 5 unidentified open reading frames (ORFs). The noncoding DNA represents 6.50 % of the total genome. The average size of intergenic spacers is 44.2 nucleotides with a range of 0 to 389 nucleotides. The nad11 gene in P. fascia, unlike other coding genes with a methionine (ATG) start codon, commences with a typical TTG codon. The P. fascia mt genome exhibits novel genome organization and gene arrangement, which are similar to those of Ectocarpus siliculosus. Phylogenetic analyses based on 35 protein-coding genes reveal that P. fascia has a close evolutionary relationship with E. siliculosus compared with other brown algae analyzed. The molecular data presented provide a useful tool for classification, evolutionary as well as population genetic studies in Scytosiphonaceae.

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Acknowledgments

The authors thank Claire M.M. Gachon and two anonymous reviewers for their helpful advice, and Jiaqi Wang, Xige Jiang, and Changbin Sun for their assistance in algal collection. This investigation was supported by the National Natural Science Foundation of China (No. 41206146), the 863 Hi-Tech Research and Development Program of China (No. 2012AA10A413), the Scientific Research Foundation for Outstanding Young Scientists of Shandong Province (No. BS2013HZ004), the Open Research Fund of Key Laboratory of Integrated Marine Monitoring and Applied Technologies for Harmful Algal Blooms, State Oceanic Administration (No. MATHAB201408), and the Open Research Fund of Key Laboratory of East China Sea and Oceanic Fishery Resources Exploitation and Utilization, Ministry of Agriculture, P. R. China (No. K201311).

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  1. Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, 7 Nanhai Road, Qingdao, 266071, People’s Republic of China

    Feng Liu & Shaojun Pang

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  1. Feng Liu
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Correspondence to Feng Liu or Shaojun Pang.

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Liu, F., Pang, S. Mitochondrial phylogenomics reveals a close relationship between Petalonia fascia (Scytosiphonaceae, Phaeophyceae) and Ectocarpus siliculosus . J Appl Phycol 27, 1021–1028 (2015). https://doi.org/10.1007/s10811-014-0386-3

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