Journal of Applied Phycology

, Volume 26, Issue 4, pp 1833–1844 | Cite as

Comparative production and nutritional value of “sea grapes” — the tropical green seaweeds Caulerpa lentillifera and C. racemosa

  • Nicholas A. PaulEmail author
  • Nicolas Neveux
  • Marie Magnusson
  • Rocky de Nys


“Sea grapes” is a collective term for the edible varieties of the green seaweed genus Caulerpa. Here we conduct comparative analyses of the biomass productivities and biochemical properties of C. lentillifera and C. racemosa from tropical Australia. Commercial-scale production was evaluated using 1 m2 culture units with high stocking densities (>5 kg m−2). Productivity of C. lentillifera in a 6-week period yielded, on average, 2 kg week−1, whereas C. racemosa yielded <0.5 kg week−1. Morphometric comparisons of the harvestable biomass revealed that C. lentillifera had a higher proportion of fronds (edible portions) to horizontal runners (stolons) and a higher density of fronds per unit area. C. racemosa fronds, however, were significantly longer. The nutritional value of C. racemosa was higher than C. lentillifera for both polyunsaturated fatty acids (10.6 vs. 5.3 mg g−1 DW) and pigments (9.4 vs. 4.2 mg g−1 DW). The content of eicosapentaenoic acid (EPA) and β-carotene decreased with increasing frond size in both species. Trace element contents also varied substantially between the species, including higher levels of zinc, magnesium and strontium in C. lentillifera, and higher levels of selenium in C. racemosa. Some less desirable elements were higher in C. lentillifera, including arsenic (1 vs. 0.1 ppm) and cadmium, whereas others were higher in C. racemosa, including lead, copper and vanadium. Overall C. lentillifera has a high biomass production potential in monoculture and distinct nutritional properties that warrant a focus on its commercialisation as a new aquaculture product in tropical Australia and in Southeast Asia more broadly.


Algae Aquaculture β-Carotene Minerals Nutrition Polyunsaturated fatty acids (PUFA) 



This research was funded by the Australian Flora Foundation. The authors thank I. Tuart (JCU) for assistance in production experiments and Y. Hu (Advanced Analytical Centre, JCU) for conducting the elemental analyses, and two anonymous reviewers for their input.

Supplementary material

10811_2013_227_MOESM1_ESM.doc (84 kb)
ESM 1 (DOC 84 kb)


  1. Barr NG, Kloeppel A, Rees TAV, Scherer C, Taylor RB, Wenzel A (2008) Wave surge increases rates of growth and nutrient uptake in the green seaweed Ulva pertusa maintained at low bulk flow velocities. Aquat Biol 3:179–186CrossRefGoogle Scholar
  2. Baumgartner FA, Motti CA, de Nys R, Paul NA (2009) Feeding preferences and host associations of specialist marine herbivores align with quantitative variation in seaweed secondary metabolites. Mar Ecol Prog Ser 396:1–12CrossRefGoogle Scholar
  3. Bocanegra A, Bastida S, Benedí J, Ródenas S, Sánchez-Muniz FJ (2009) Characteristics and nutritional and cardiovascular-health properties of seaweeds. J Med Food 12:236–258PubMedCrossRefGoogle Scholar
  4. Bracken MES, Stachowicz JJ (2006) Seaweed diversity enhances nitrogen uptake via complementary use of nitrate and ammonium. Ecology 87:2397–2403PubMedCrossRefGoogle Scholar
  5. Carvalho AP, Malcata FX (2005) Preparation of fatty acid methyl esters for gas-chromatographic analysis of marine lipids: insight studies. J Agr Food Chem 53:5049–5059CrossRefGoogle Scholar
  6. Cohen Z, Vonshak A, Richmond A (1988) Effect of environmental conditions on fatty acid composition of the red alga Porphyridium cruentum: Correlation to growth-rate. J Phycol 24:328–332Google Scholar
  7. David F, Sandra P, Wylie PL (2002) Agilent Application note 5988-5871EN. Improving the analysis of fatty acid methyl esters using retention time locked methods and retention time databases. Agilent Technologies IncGoogle Scholar
  8. Dawczynski C, Schaefer U, Leiterer M, Jahreis G (2007a) Nutritional and toxicological importance of macro, trace, and ultra-trace elements in algae food products. J Agr Food Chem 55:10470–10475CrossRefGoogle Scholar
  9. Dawczynski C, Schubert R, Jahreis G (2007b) Amino acids, fatty acids, and dietary fibre in edible seaweed products. Food Chem 103:891–899CrossRefGoogle Scholar
  10. Ferruzzi MG, Bohm V, Courtney PD, Schwartz SJ (2002) Antioxidant and antimutagenic activity of dietary chlorophyll derivatives determined by radical scavenging and bacterial reverse mutagenesis assays. J Food Sci 67:2589–2595CrossRefGoogle Scholar
  11. Food and Nutrition Board USA (1981) Food chemical codex, 3rd edn. National Academy Press, WashingtonGoogle Scholar
  12. Galland-Irmouli AV, Fleurence J, Lamghari R, Lucon M, Rouxel C, Barbaroux O, Bronowicki JP, Villaume C, Gueant JL (1999) Nutritional value of proteins from edible seaweed Palmaria palmata (Dulse). J Nutr Biochem 10:353–359PubMedCrossRefGoogle Scholar
  13. Garcia-Gonzalez M, Moreno J, Manzano JC, Florencio FJ, Guerrero MG (2005) Production of Dunaliella salina biomass rich in 9-cis-beta-carotene and lutein in a closed tubular photobioreactor. J Biotechnol 115:81–90PubMedCrossRefGoogle Scholar
  14. Gosch BJ, Magnusson M, Paul NA, de Nys R (2012) Total lipid and fatty acid composition of seaweeds for the selection of species for oil-based biofuel and bioproducts. Gcb Bioenergy 4:919–930CrossRefGoogle Scholar
  15. Griffiths MJ, Harrison STL (2009) Lipid productivity as a key characteristic for choosing algal species for biodiesel production. J Appl Phycol 21:493–507CrossRefGoogle Scholar
  16. Holdt SL, Kraan S (2011) Bioactive compounds in seaweed: functional food applications and legislation. J Appl Phycol 23:543–597CrossRefGoogle Scholar
  17. Horstmann U (1983) Cultivation of the green alga, Caulerpa racemosa, in tropical waters and some aspects of its physiological ecology. Aquaculture 32:361–371CrossRefGoogle Scholar
  18. Huang L, Wen KW, Gao X, Liu YH (2010) Hypolipidemic effect of fucoidan from Laminaria japonica in hyperlipidemic rats. Pharm Biol 48:422–426PubMedCrossRefGoogle Scholar
  19. Hurd CL (2000) Water motion, marine macroalgal physiology, and production. J Phycol 36:453–472CrossRefGoogle Scholar
  20. Indergaard M, Minsaas J (1991) Animal and human nutrition. In: Guiry MD, Blunden G (eds) Seaweed resources in Europe. Wiley, Chichester, pp 21–64Google Scholar
  21. Kumar M, Kumari P, Trivedi N, Shukla MK, Gupta V, Reddy CRK, Jha B (2011) Minerals, PUFAs and antioxidant properties of some tropical seaweeds from Saurashtra coast of India. J Appl Phycol 23:797–810CrossRefGoogle Scholar
  22. Kumari P, Kumar M, Gupta V, Reddy CRK, Jha B (2010) Tropical marine macroalgae as potential sources of nutritionally important PUFAs. Food Chem 120:749–757CrossRefGoogle Scholar
  23. Lawton RJ, de Nys R, Paul NA (2013) Selecting reliable and robust freshwater macroalgae for biomass applications. PloS one 8(5):e64168Google Scholar
  24. Lüning K, Pang SJ (2003) Mass cultivation of seaweeds: current aspects and approaches. J Appl Phycol 15:115–119CrossRefGoogle Scholar
  25. MacArtain P, Gill CIR, Brooks M, Campbell R, Rowland IR (2007) Nutritional value of edible seaweeds. Nutr Rev 65:535–543PubMedCrossRefGoogle Scholar
  26. Magnusson M, Mata L, de Nys R, Paul NA (2013) Biomass, lipid and fatty acid production in large-scale cultures of the marine macroalga Derbesia tenuissima (Chlorophyta). Mar BiotechGoogle Scholar
  27. Marsham S, Scott GW, Tobin ML (2007) Comparison of nutritive chemistry of a range of temperate seaweeds. Food Chem 100:1331–1336CrossRefGoogle Scholar
  28. Matanjun P, Mohamed S, Muhammad K, Mustapha NM (2010) Comparison of cardiovascular protective effects of tropical seaweeds, Kappaphycus alvarezii, Caulerpa lentillifera, and Sargassum polycystum, on high-cholesterol/high-fat diet in rats. J Med Food 13:792–800PubMedCrossRefGoogle Scholar
  29. Matanjun P, Mohamed S, Mustapha NM, Muhammad K (2009) Nutrient content of tropical edible seaweeds, Eucheuma cottonii, Caulerpa lentillifera and Sargassum polycystum. J Appl Phycol 21:75–80CrossRefGoogle Scholar
  30. Murata M, Ishihara K, Saito H (1999) Hepatic fatty acid oxidation enzyme activities are stimulated in rats fed the brown seaweed, Undaria pinnatifida (wakame). J Nutr 129:146–151PubMedGoogle Scholar
  31. Ortega-Calvo JJ, Mazuelos C, Hermosin B, Saizjimenez C (1993) Chemical composition of Spirulina and eukaryotic algae food products marketed in Spain. J Appl Phycol 5:425–435CrossRefGoogle Scholar
  32. Ostraff M (2006) Limu: edible seaweed in Tonga, an ethnobotanical study. J Ethnobiol 26:208–227CrossRefGoogle Scholar
  33. Patarra R, Paiva L, Neto AI, Lima E, Baptista J (2011) Nutritional value of selected macroalgae. J Appl Phycol 23:205–208CrossRefGoogle Scholar
  34. Paul NA, de Nys R (2008) Promise and pitfalls of locally abundant seaweeds as biofilters for integrated aquaculture. Aquaculture 281:49–55CrossRefGoogle Scholar
  35. Paul NA, de Nys R (2011) Cultivating seaweed. Australian Patent Application AU2010224354Google Scholar
  36. Paul NA, Tseng CK (2012) Seaweed. In: Lucas JS, Southgate PC (eds) Aquaculture: farming aquatic animals and plants, vol 2. Blackwell Publishing, Oxford, pp 268–284Google Scholar
  37. Peña-Rodriguez A, Mawhinney TP, Ricque-Marie D, Cruz-Suarez LE (2011) Chemical composition of cultivated seaweed Ulva clathrata (Roth) C. Agardh. Food Chem 129:491–498CrossRefGoogle Scholar
  38. Poudyal H, Panchal SK, Ward LC, Brown L (2013) Effects of ALA, EPA and DHA in high-carbohydrate, high-fat diet-induced metabolic syndrome in rats. J Nutr Biochem 24:1041–1052PubMedCrossRefGoogle Scholar
  39. Rangel-Yagui CD, Danesi EDG, de Carvalho JCM, Sato S (2004) Chlorophyll production from Spirulina platensis: cultivation with urea addition by fed-batch process. Biores Technol 92:133–141Google Scholar
  40. Roberts DA, de Nys R, Paul NA (2013) The effect of CO2 on algal growth in industrial waste water for bioenergy and bioremediation applications. PloS one 8(11):e81631PubMedCentralPubMedCrossRefGoogle Scholar
  41. Rose M, Lewis J, Langford N, Baxter M, Origgi S, Barber M, MacBain H, Thomas K (2007) Arsenic in seaweed—forms, concentration and dietary exposure. Food Chem Toxicol 45:1263–1267PubMedCrossRefGoogle Scholar
  42. Rupérez P (2002) Mineral content of edible marine seaweeds. Food Chem 79:23–26CrossRefGoogle Scholar
  43. Saito H, Xue CH, Yamashiro R, Moromizato S, Itabashi Y (2010) High polyunsaturated fatty acid levels in two subtropical macroalgae, Cladosiphon okamuranus and Caulerpa lentillifera. J Phycol 46:665–673CrossRefGoogle Scholar
  44. Saunders RJ, Paul NA, Hu Y, de Nys R (2012) Sustainable sources of biomass for bioremediation of heavy metals in waste water derived from coal-fired power generation. PloS one 7 (5):e36470Google Scholar
  45. Shahidi F (2009) Nutraceuticals and functional foods: whole versus processed foods. Trends Food Sci Tech 20:376–387CrossRefGoogle Scholar
  46. Simopoulos AP (2002) The importance of the ratio of omega-6/omega-3 essential fatty acids. Biomed Pharmacother 56:365–379PubMedGoogle Scholar
  47. South GR (1993) Edible seaweeds of Fiji — an ethnobotanical study. Bot Mar 36:335–349CrossRefGoogle Scholar
  48. Van Heukelem L, Thomas CS (2001) Computer-assisted high-performance liquid chromatography method development with applications to the isolation and analysis of phytoplankton pigments. J Chromatogr A 910:31–49PubMedCrossRefGoogle Scholar
  49. Wong KH, Cheung PCK (2000) Nutritional evaluation of some subtropical red and green seaweeds: Part I. Proximate composition, amino acid profiles and some physico-chemical properties. Food Chem 71:475–482CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Nicholas A. Paul
    • 1
    Email author
  • Nicolas Neveux
    • 1
  • Marie Magnusson
    • 1
  • Rocky de Nys
    • 1
  1. 1.MACRO — the Centre for Macroalgal Resources and Biotechnology, and School of Marine and Tropical BiologyJames Cook UniversityQueenslandAustralia

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