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Triacylglycerol content, productivity and fatty acid profile in Scenedesmus acutus PVUW12

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Abstract

A detailed lipid characterization of Scenedesmus acutus PVUW12, with emphasis on the evaluation of triacylglycerols (TAGs) as a biodiesel feedstock, is presented. When algal cells were grown in nitrogen-free medium (N stress), a lipid increase was detected that was mainly due to TAG accumulation. In situ fluorescence measurements allowed the kinetics and extent of neutral lipid accumulation to be followed. Under N stress, the productivity of total lipids and TAGs increased significantly (80.99 and 63.74 mg L−1 day−1, respectively) compared with controls (29.51 and 16.23 mg L−1 day−1, respectively). Monounsaturated fatty acids were the major fraction and increased further (49.74 %) in stressed cells, with oleic acid as the most abundant compound (46.97 %). The polyunsaturated fatty acid composition of this algal oil appears to meet the European Standard EN 14214. These results indicate that S. acutus oil meets the requirements for its use as a biodiesel feedstock. Since this strain was also proposed for wastewater bioremediation, this opens up the possibility of its use in an integrated system combined with biofuel production.

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Acknowledgments

The authors thank the Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET) de la República Argentina, PIP 112-200801-00234; the Secretaría de Ciencia y Tecnología de la Universidad Nacional del Sur, PGI TIR 24/TB08; Consorzio Universitario Italiano per l’Argentina (CUIA) 2009; and the Agencia Nacional de Promoción Científica y Tecnológica, PICT 2010-0959. This work was also supported by a generous grant from the Fondazione Bussolera-Branca. P.I.L. is a research member of CONICET.

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Correspondence to Patricia I. Leonardi.

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Damiani, M.C., Popovich, C.A., Constenla, D. et al. Triacylglycerol content, productivity and fatty acid profile in Scenedesmus acutus PVUW12. J Appl Phycol 26, 1423–1430 (2014). https://doi.org/10.1007/s10811-013-0170-9

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  • DOI: https://doi.org/10.1007/s10811-013-0170-9

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