Morphological, ultrastructural, and genetic characterization of coalescence in the intertidal and shallow subtidal kelps Lessonia spicata and L. berteroana (Laminariales, Heterokontophyta)

Abstract

Coalescing macroalgae may fuse with conspecifics, forming genetically heterogeneous entities known as chimera. This process has been shown in taxa from roughly half the red algal orders and in the Codium species, a green alga. Field observations indicate that common and dominant kelps along central Chile exhibit a fused holdfast. We evaluated whether such fusions are true coalescence processes in Lessonia spicata and Lessonia berteroana. To this end, we characterized the ultrastructural event involved in holdfast fusion in the laboratory. Additionally, coalescence in natural populations was quantified by measuring the frequency of individuals with genetically heterogenic stipes within the same holdfast. Results indicate that coalescence appears as a frequent process in laboratory, mostly restricted to intraspecific fusions. During fusion, the meristodermatic cells located in the contact area modify their morphology and reduce the number of plastids, mitochondria, and cell inclusions. The cell wall becomes much thinner and develops plasmodesmata, enhancing communication with equivalent cells of the other coalescencing individual. Stipe genotyping indicates that there is a widespread occurrence of chimerism in both species and genetic heterogeneity is increasing directly with the increasing number of stipes. The combination of results suggests that kelp frequently coalesce in the field, and the histological response observed approaches that of red algae. Since kelps are part of the dominant vegetation in low intertidal and shallow subtidal beds, the adaptive values of coalescence in these species should be evaluated. It is concluded that coalescence and chimerism are evolutionary convergent processes, occurring in all three major groups of seaweeds.