Microalgae growth on concentrated human urine
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In this study, for the first time, a microalga was grown on non-diluted human urine. The essential growth requirements for the species Chlorella sorokiniana were determined for different types of human urine (fresh, hydrolysed, male and female). Batch experimental results using microtiter plates showed that both fresh and synthetic urine supported rapid growth of this species, provided additional trace elements (Cu, Fe, Mn, and Zn) were added. When using hydrolysed urine instead of fresh urine, additional magnesium had to be added as it precipitates during hydrolysis of urea. C. sorokiniana was able to grow on non-diluted urine with a specific growth rate as high as 0.104 h−1 under light-limited conditions (105 μmol photons m−2 s−1), and the growth was not inhibited by ammonium up to a concentration of 1,400 mg NH4 +-N L−1. The highest growth rate on human urine was as high as 0.158 h−1. Because it was demonstrated that concentrated urine is a rich and good nutrient source for the production of microalgae, its application for a large-scale economical and sustainable microalgae production for biochemicals, biofuels and biofertilizers becomes feasible.
KeywordsMicroalgae growth Source-separated urine Trace elements Magnesium
The project is financially supported by Innowater funding provided by the Dutch Department of Economic Affairs. The Ph.D. student is financially granted by the Ministry of Science and Technology, Thailand.
- APHA (1998) Standard methods for the examination of water and wastewater, 22nd edn. American Public Health Association, Washington D.CGoogle Scholar
- Azov Y, Goldman JC (1982) Free ammonia inhibition of algal photosynthesis in intensive cultures. Appl Environ Microb 43:735–739Google Scholar
- Eyster C (1978) Nutrient concentration requirements for Chlorella Sorokiniana. Ohio J Sci 78:79–81Google Scholar
- Oh-Hama T, Miyachi S (1988) Chlorella. In: Borowitzka MA, Borowitzka LJ (eds) Micro-algal biotechnology. Cambridge University Press, Cambridge, pp 3–26Google Scholar
- Redfield AC (1958) The biological control of chemical factors in the environment. Am Sci 230A:205–221Google Scholar
- Tsalev DL (1984) Atomic absorption spectrometry in occupational and environmental health practice. Volume II: Determination of individual elements. CRC Press Inc, Boca RatonGoogle Scholar
- Zeeman G, Kujawa K, Mes T, Hernandez H, Graaff M, Abu-Ghunmi L, Mels A, Meulman B, Temmink H, Buisman C, Van Lier J, Lettinga G (2008) Anaerobic treatment as a core technology for energy, nutrients and water recovery from source-separated domestic waste(water). Water Sci Technol 57:1207–1212PubMedCrossRefGoogle Scholar