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Potential of Candida utilis to ferment Ecklonia cava by-product for enhanced anti-methicillin-resistant Staphylococcus aureus (MRSA) activity

Abstract

In order to utilize the by-product of Ecklonia cava (the remaining biomass of E. cava), microbial fermentation which may result in the production of bioactive compounds using the by-product was applied in this study. The fermentation broth of E. cava by-product fermented with Candida utilis showed enhanced antibacterial activity against methicillin-resistant Staphylococcus aureus (MRSA) and food-borne pathogenic bacteria compared to that of control. To perform a more detailed investigation on the antibacterial activity, the broth was extracted with methanol and further fractionated with organic solvents. After 1 day of fermentation, the ethyl acetate (EtOAc) fraction exhibited the highest anti-MRSA activity with minimum inhibitory concentration ranging from 64 to 256 μg mL−1, suggesting that the fermentation of E. cava by-product with C. utilis could enhance antibacterial activity against MRSA. In addition, high-performance liquid chromatography analysis revealed that dieckol, eckol, eckstolonol, and triphlorethol-A contents in the EtOAc-soluble extract increased significantly. The anti-MRSA activity of E. cava by-product most probably originated from phlorotannins, and the fermentation of C. utilis may have stimulated the breakdown of phlorotannins or have increased the efficiency in extracting phlorotannins.

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Acknowledgments

This work was supported by a research grant from Pukyong National University (2013).

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Correspondence to Young-Mog Kim.

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Sung-Hwan Eom and Keun-Sik Lim equally contributed to this work.

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Eom, SH., Lim, KS. & Kim, YM. Potential of Candida utilis to ferment Ecklonia cava by-product for enhanced anti-methicillin-resistant Staphylococcus aureus (MRSA) activity. J Appl Phycol 25, 1949–1956 (2013). https://doi.org/10.1007/s10811-013-0034-3

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  • DOI: https://doi.org/10.1007/s10811-013-0034-3

Keywords

  • Antimicrobial activity
  • Candida utilis
  • Ecklonia cava by-product
  • Fermentation
  • HPLC
  • MRSA