Journal of Applied Phycology

, Volume 25, Issue 6, pp 1793–1803 | Cite as

In vitro assessment of the cardioprotective, anti-diabetic and antioxidant potential of Palmaria palmata protein hydrolysates

  • Pádraigín A. Harnedy
  • Richard J. FitzGeraldEmail author


The contribution of protein fraction and proteolytic enzyme preparation to the in vitro cardioprotective, anti-diabetic and antioxidant activity of Palmaria palmata protein hydrolysates was investigated. Aqueous, alkaline and combined aqueous and alkaline P. palmata protein fractions were hydrolysed with the food-grade proteolytic preparations, Alcalase 2.4 L, Flavourzyme 500 L and Corolase PP. The hydrolysates had angiotensin converting enzyme (ACE) and dipeptidyl peptidase (DPP) IV inhibitory activity with IC50 values in the range 0.19–0.78 and 1.65–4.60 mg mL−1, respectively. The oxygen radical absorbance capacity (ORAC) and ferric reducing antioxidant power (FRAP) values ranged from 45.17 to 467.54 and from 1.06 to 21.59 μmol trolox equivalents/g, respectively. Furthermore, hydrolysates (1 mg mL−1) were show to inhibit renin within the range 0–50 %. In general, Alcalase 2.4 L and Corolase PP hydrolysates of aqueous protein displayed the highest in vitro activity. The results indicate that protein fraction and enzyme preparation used have significant effects on in vitro biofunctional activity of the hydrolysates. This study demonstrates the potential of P. palmata protein hydrolysates as multifunctional functional food ingredients for the prevention/control of hypertension and type II diabetes.


Palmaria palmata Protein hydrolysates Bioactive peptides Angiotensin converting enzyme Renin Dipeptidyl peptidase IV 



This project (Grant Aid Agreement No. MFFRI/07/01) was carried out under the Sea Change Strategy with the support of the Marine Institute and the Department of Agriculture, Food and the Marine, funded under the National Development Plan 2007–2013. The authors would like to acknowledge Dr. Anna Soler-Vila, NUI Galway for the freeze-dried P. palmata sample.


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Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Pádraigín A. Harnedy
    • 1
  • Richard J. FitzGerald
    • 1
    Email author
  1. 1.Department of Life SciencesUniversity of LimerickLimerickIreland

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