In vitro assessment of the cardioprotective, anti-diabetic and antioxidant potential of Palmaria palmata protein hydrolysates
- 1.5k Downloads
The contribution of protein fraction and proteolytic enzyme preparation to the in vitro cardioprotective, anti-diabetic and antioxidant activity of Palmaria palmata protein hydrolysates was investigated. Aqueous, alkaline and combined aqueous and alkaline P. palmata protein fractions were hydrolysed with the food-grade proteolytic preparations, Alcalase 2.4 L, Flavourzyme 500 L and Corolase PP. The hydrolysates had angiotensin converting enzyme (ACE) and dipeptidyl peptidase (DPP) IV inhibitory activity with IC50 values in the range 0.19–0.78 and 1.65–4.60 mg mL−1, respectively. The oxygen radical absorbance capacity (ORAC) and ferric reducing antioxidant power (FRAP) values ranged from 45.17 to 467.54 and from 1.06 to 21.59 μmol trolox equivalents/g, respectively. Furthermore, hydrolysates (1 mg mL−1) were show to inhibit renin within the range 0–50 %. In general, Alcalase 2.4 L and Corolase PP hydrolysates of aqueous protein displayed the highest in vitro activity. The results indicate that protein fraction and enzyme preparation used have significant effects on in vitro biofunctional activity of the hydrolysates. This study demonstrates the potential of P. palmata protein hydrolysates as multifunctional functional food ingredients for the prevention/control of hypertension and type II diabetes.
KeywordsPalmaria palmata Protein hydrolysates Bioactive peptides Angiotensin converting enzyme Renin Dipeptidyl peptidase IV
This project (Grant Aid Agreement No. MFFRI/07/01) was carried out under the Sea Change Strategy with the support of the Marine Institute and the Department of Agriculture, Food and the Marine, funded under the National Development Plan 2007–2013. The authors would like to acknowledge Dr. Anna Soler-Vila, NUI Galway for the freeze-dried P. palmata sample.
- Boots JWP (2006) Protein hydrolysates enriched in peptides inhibiting DPP-IV and their use. PCT Patent Application WO/2006/068480Google Scholar
- Cian RE, Alaiz M, Vioque J, Drago SR (2012) Enzyme proteolysis enhanced extraction of ACE inhibitory and antioxidant compounds (peptides and polyphenols) from Porphyra columbina residual cake. J Appl Phycol. doi: 10.1007/s10811-012-9913-2
- Meisel H, Walsh DJ, Murray BA, FitzGerald RJ (2006) ACE inhibibtory peptides. In: Mine Y, Shahidi F (eds) Nutraceutical proteins and peptides in health and disease, vol 4. CRC Press, Boca Raton, pp 269–315Google Scholar
- Sato M, Hosokawa T, Yamaguchi T, Nakano T, Muramoto K, Kahara T, Funayamab K, Kobayashib A, Nakano T (2002a) Angiotensin I-converting enzyme inhibitory peptides derived from brown alga (Undaria pinnatifida) and their antihypertensive effect in spontaneously hypertensive rats. J Agric Food Chem 50:6245–6252PubMedCrossRefGoogle Scholar
- Van Amerongen A, Beelen-Thomissen MJC, Van Zeeland-Wolbers LAM, Van Gilst WH, Buikema JH, Nelissen JWPM (2009) Egg protein hydrolysates. PCT Patent Application WO/2009/128713Google Scholar