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Cryopreservation of gametophytic thalli of Porphyra yezoensis (Rhodophyceae) by one-step fast cooling

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Abstract

This study investigated the toxicity and effectiveness of various cryoprotectant solutions to develop a cryopreservation technique by one-step fast cooling for gametophytic thalli of Porphyra yezoensis. Dimethyl sulfoxide (DMSO) was not harmful at concentrations up to 30 % after 30 s of immersion. However, an increase in concentration beyond 40 % was seriously detrimental, and the detrimental effect occurred at lower concentrations in the case of 60 s of immersion. Diglycerol was the least harmful among cryoprotectants tested, and its harmful effect was not found at concentrations up to 50 %, even after 60 s of immersion. The thalli loaded into a straw were directly plunged into liquid nitrogen after 30 s of immersion in various cryoprotectant solutions. When 5 % DMSO was added to diglycerol, high survival rates (more than 80 %) were achieved at diglycerol concentrations of 15–25 %, and the maximum survival was 94.6 % at diglycerol concentrations of 20 %. The thawed thalli restarted growth without any difference in comparison with unfrozen thalli.

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Acknowledgments

This study was supported in part by a grant-in-aid for scientific research (no. 22580379) to K.K. from the Ministry of Education, Science and Culture, Japan.

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Authors and Affiliations

  1. Fisheries Science Research Center, Pukyong National University, Busan, 619-911, South Korea

    Youn Hee Choi

  2. Department of Food Science and Nutrition, Pukyong National University, Busan, 608-737, South Korea

    Taek Jeong Nam

  3. Graduate School of Fisheries Science and Environmental Studies, Nagasaki University, Nagasaki, 852-8521, Japan

    Kazuyoshi Kuwano

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  1. Youn Hee Choi
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  2. Taek Jeong Nam
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  3. Kazuyoshi Kuwano
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Correspondence to Youn Hee Choi.

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Choi, Y.H., Nam, T.J. & Kuwano, K. Cryopreservation of gametophytic thalli of Porphyra yezoensis (Rhodophyceae) by one-step fast cooling. J Appl Phycol 25, 531–535 (2013). https://doi.org/10.1007/s10811-012-9887-0

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  • DOI: https://doi.org/10.1007/s10811-012-9887-0

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