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Lactic acid production from seaweed hydrolysate of Enteromorpha prolifera (Chlorophyta)

Abstract

We examined the feasibility of using the green seaweed Enteromorpha prolifera as an alternative carbon source for chemical production. For this purpose, the chemical composition (proximate analysis, ultimate analysis, and mineral analysis) and acid hydrolysis of E. prolifera were investigated. In addition, lactic acid fermentation of E. prolifera hydrolysate was carried out using five Lactobacillus strains. The lactic acid yield, which is defined as the ratio of the lactic acid production to total sugar consumption, varied depending on the strains. Lactobacillus salivarius showed the highest lactic acid yield (68.5%), followed by Lactobacillus plantarum (66.0%), Lactobacillus rhamnosus (55.8%), Lactobacillus brevis (54.5%), and Lactobacillus casei (51.4%). The results shown in this study imply that E. prolifera would be competitive with lignocellulosic biomass such as corn stover in terms of lactic acid production yield and that green seaweed can be used as a feedstock for industrial production of chemicals.

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References

  • Biermann CJ, McGinnis GD (1988) Analysis of carbohydrates by GLC and MS. CRS Press, Florida, pp 32–33

    Google Scholar 

  • Chapman VJ, Chapman DJ (1980) Seaweeds and their use, 3rd edn. Chapman & Hall, London, pp 88–89

    Book  Google Scholar 

  • Cho ML, Yang CY, Kim SM, You SG (2010) Molecular characterization and biological activities of water soluble sulfated polysaccharides from Enteromorpha prolifera. Food Sci Biotechnol 19:525–533

    Article  CAS  Google Scholar 

  • Cui F, Li Y, Wan C (2011) Lactic acid production from corn stover using mixed cultures of Lactobacillus rhamnosus and Lactobacillus brevis. Bioresour Technol 102:1831–1836

    PubMed  Article  CAS  Google Scholar 

  • Korea Food and Drug Administration (2009) The Korea Food Code. Vol. 2, Chapter 10, pp 1–53

  • Garde A, Jonsson G, Schmidt AS, Ahring BK (2002) Lactic acid production from wheat straw hemicelluloses hydrolysate by Lactobacillus pentosus and Lactobacillus brevis. Bioresour Technol 81:217–223

    PubMed  Article  CAS  Google Scholar 

  • Hofvendahl K, Hahn-Hagerdal B (2000) Factors affecting the fermentative lactic acid production from renewable resources. Enzyme Microb Technol 26:87–107

    PubMed  Article  CAS  Google Scholar 

  • John RP, Nampoothiri KM, Pandey A (2007) Fermentative production of lactic acid from biomass: and overview on process developments and future perspectives. Appl Microbiol Biotechnol 74:524–534

    PubMed  Article  CAS  Google Scholar 

  • Li Z, Ding S, Li Z, Tan T (2006) L-lactic acid production by Lactobacillus casei fermentation with corn steep liquor-supplemented acid-hydrolysate of soybean meal. Biotechnol J 1:1453–1458

    PubMed  Article  CAS  Google Scholar 

  • Li Z, Han L, Ji Y, Wang X, Tan T (2010) Fermentative production of l-lactic acid from hydrolysate of wheat bran by Lactobacillus rhamnosus. Biochem Eng J 49:138–142

    Article  CAS  Google Scholar 

  • Miller L, Houghton JA (1945) The micro-Kjeldahl determination of the nitrogen content of amino acids and proteins. J Biol Chem 159:373–383

    CAS  Google Scholar 

  • Okano K, Tanaka T, Ogino C, Fukuda H, Kondo A (2010) Biotechnological production of enantiomeric pure lactic acid from renewable resources: recent achievements, perspectives, and limits. Appl Microbiol Biotechnol 85:413–423

    PubMed  Article  CAS  Google Scholar 

  • Oude Elferink SJWH, Krooneman J, Gottschal JC, Spoelstra SF, Faber F, Driehuis F (2001) Anaerobic conversion of lactic acid to acetic acid and 1,2-propanediol by Lactobacillus buchneri. Appl Environ Microbiol 67:125–132

    PubMed  Article  CAS  Google Scholar 

  • Roesijad G, Jones SB, Snowden-Swan LJ, Zhu Y (2010) Macroalgae as a biomass feedstock: a preliminary analysis, PNNL 19944. Pacific Northwest National Laboratory, USA

    Book  Google Scholar 

  • Roy D, Goulet J, LeDuy A (1986) Batch fermentation of whey ultrafiltrate by Lactobacillus helveticus for lactic acid production. Appl Microbiol Biotechnol 24:206–213

    Article  CAS  Google Scholar 

  • Saxena RK, Anand P, Saran S, Isar J, Agarwal L (2010) Microbial production and applications of 1, 2-propanediol. Indian J Microbiol 50:2–11

    Article  CAS  Google Scholar 

  • Tsuji H, Ikada Y (1992) Stereocomplex formation between enantiomeric poly(lactic acid)s. 6. Binary blends from copolymers. Macromolecules 25:5719–5723

    Article  CAS  Google Scholar 

  • Wee YJ, Kim JN, Ryu HW (2006) Biotechnological production of lactic acid and its recent applications. Food Technol Biotech 44:163–172

    CAS  Google Scholar 

Download references

Acknowledgment

This work was supported by the Marine Biotechnology Program of Korean Ministry of Land, Transport and Maritime Affairs.

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Correspondence to Sun Bok Lee.

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Hwang, H.J., Kim, S.M., Chang, J.H. et al. Lactic acid production from seaweed hydrolysate of Enteromorpha prolifera (Chlorophyta). J Appl Phycol 24, 935–940 (2012). https://doi.org/10.1007/s10811-011-9714-z

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  • DOI: https://doi.org/10.1007/s10811-011-9714-z

Keywords

  • Enteromorpha prolifera
  • Seaweed
  • Lactic acid fermentation
  • Lactobacillus
  • Chemical composition
  • Acid hydrolysis