Abstract
Experimental ecology methods and chlorophyll fluorescence technology were used to study the effects of different concentrations of manganese (10−12– 10−4 mol L−1) on the growth, photosystem II and superoxide dismutase (SOD) activity of Amphidinium sp. MACC/D31. The results showed that manganese had a significant effect on the growth rate, fluorescence parameters (maximal photochemical efficiency of PSII (F v /F m ), photochemical quenching (qP) and non-photochemical quenching (NPQ)) in the exponential stage (days 1–3) and SOD activity of Amphidinium sp. (P < 0.05). F v/F m in the exponential stage in 10−12 mol L−1 manganese concentration was significantly lower whilst qP and NPQ significantly higher than those in the other concentrations. F v /F m (days 6–9) in 10−4 mol L−1 manganese was significantly higher than those in the other concentrations. F v /F m (days 3–6) increased with increased concentration of manganese from 10−12 to 10−4 mol L−1. The values of qP and NPQ decreased with decreased concentrations of manganese, except for those in days 4–6. F v /F m under each concentration increased earlier and decreased later with culture stage whilst NPQ decreased earlier and increased later. The SOD activity increased with increased concentration of manganese from 10−12 to 10−8 mol L−1. The SOD activity in 10−4 mol L−1 manganese was significantly higher than those in the other concentrations and in 10−12 mol L−1 manganese, it was significantly lower than those in the other concentrations.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Allen MD, Kropat J, Tottey S, Campo JAD, Merchant SS (2007) Manganese deficiency in Chlamydomonas results in loss of photosystem II and MnSOD function, sensitivity to peroxides, and secondary phosphorus and iron deficiency. Plant Physiol 143:263–277
Brand LE, Sunda WG, Guillard RRL (1983) Limitation of marine phytoplankton reproductive rates by zinc, manganese, and iron. Limnol Oceanogr 28(6):1182–1198
Cai HJ, Tang XX, Zhang PY, Xiao H, Yu J (2006) Response of activity of antioxidant enzymes to UV-B radiation enhancement in three species of red-tide microalgae. Periodical Ocean Univ China 36(1):81–84
Ferroni L, Baldisserotto C, Fasulo MP, Pagnoni A, Pancaldi S (2004) Adaptive modifications of the photosynthetic apparatus in Euglena gracilis Klebs exposed to manganese excess. Protoplasma 224:167–177
Gonzalez A, Steffen KL, Lynch JP (1998) Light and excess manganese. Implications for oxidative stress in common bean. Plant Physiol 118:493–504
Guillard RR, Ryther JH (1962) Studies of marine planktonic diatoms, I. Cyclotella nana Hustedt and Detonula confervacea (Cleve) Gran. Can J Microbiol 8:229–239
Han XT, Yan T, Zou JZ, Yu ZM (2004) Morphological features and growth characteristics of the dinoflagellate Amphidinium carterae Hulburk. Oceanology Limnol Sin 35(2):279–283
Huang XC, Zhao D, Guo YW, Wu HM, Lin LP, Wang ZH, Ding J, Lin YS (2004) Lingshuiol, a novel polyhydroxyl compound with strongly cytotoxic activity from the marine dinoflagellate Amphidinium sp. Bioorg Med Chem Lett 14:3117–3120
Jiang CD, Gao HY, Zou Q (2002) Energy-dependent quenching of chlorophyll fluorescence is depressed in Mn-starved soybean leaves. J Plant Physiol Mol Biol 28:287–291
Kessler E (1970) Photosynthesis, Photo oxidation of chlorophyll and fluorescence of normal and manganese-deficient Chlorella with and without hydrogenase. Planta 92:222–234
Kromkamp J, Forster R (2003) The use of variable fluorescence measurements in aquatic ecosystems differences between multiple and single turnover measuring protocols and suggested terminology. Eur J Phycol 38:103–112
Liang Y, Feng LX, Yin CL, Cao CH (2007) Current status and prospect of chlorophyll fluorescence technique in the study of responses of microalgae to environmental stress. Mar Sci 31:71–76
Lidon FC, Teixeira MG (2000) Oxy radical production and control in the chloroplast of Mn-treated rice. Plant Sci 152:7–15
Ni H, Yu HN, Liu P, Xu GD (2007) Response of photosynthetic characteristics to Mn nutrition in soybean seedling. Plant Nutr Fertilizer Sci 13:1135–1142
Olson RJ, Chisholm SW (1986) Effects of light and nitrogen limitation on the cell cycle of the dinoflagellate Amphidinium carterae. J Plankton Res 8:785–793
Paul A, Hauck M (2006) Effects of manganese on chlorophyll fluorescence in epiphytic cyano- and chlorolichens. Flora 201:451–460
Peers G, Price NM (2004) A role for manganese in superoxide dismutases and growth of iron-deficient diatoms. Limnol Oceanogr 49:1774–1783
Qiu CE, Bi YH, Hu ZY (2007) The effects of zn2+ stress on the growth, physiological characteristics and cell structure of Chlorococcum sp. Acta Hydrobiol Sin 31(4):503–508
Shi QH, Zhu ZJ, Xu M, Qian QQ, Yu JQ (2006) Effect of excess manganese on the antioxidant system in Cucumis sativus L. under two light intensities. Environ Exp Bot 58:197–205
Strasser R, Tsimilli-Michael M, Srivastava A (2004) Analysis of the chlorophyll a fluorescence transient. In: Papageorgiou G, Govindjee (eds) Chlorophyll a fluorescence: a signature of photosynthesis. Springer, Dordrecht, pp 321–362
Teichler-Zallen D (1969) The effect of manganese on chloroplast structure and photosynthetic ability of Chlamydomonas reinhardi. Plant Physiol 44:701–710
Wang H, Zhao SC, Xia WJ, Wang XB, Fan HL, Zhou W (2008) Effect of cadmium stress on photosynthesis, lipid peroxidation and antioxidant enzyme activities in maize (Zea mays L.) seedlings. Plant Nutrition Fertilizer Sci 14:36–42
Wolfe-Simon F, Grzelryk D, Schofield O, Falkowski PG (2005) The role and evolution of superoxide dismutases in algae. J Phycol 41:453–465
Yan H, Wang XJ, Lin YX, Wen G (2001) Toxic effects of Cu, Zn and Mn on the inhibition of Chlorella pyrenoidosa’s growth. Environ Sci 22:23–26
Yang ZB, You JF, Yang ZM (2007) Manganese uptake and transportation as well as antioxidant response to excess manganese in plants. J Plant Physiol Mol Biol 33:480–488
Yu Q, Rengel Z (1999) Micronutrient deficiency influences plant growth and activities of superoxide dismutases in narrow-leafed lupins. Ann Bot 83:175–182
Yu L, Zhu JW, Wu ZY, Weng XY, Gou P, Zhu GN, Chen XX (2009) Effects of glyphosate on shikimate content and antioxidant enzymes activity in Alternanthera philoxeroides under high manganese stress. Chin J Pesticide Sci 11(3):298–303
Acknowledgements
This research was funded by the State Key Development Program for Basic Research of China (No. 2007CD407306-2) and the Natural Science Foundation of Tianjin, China (No. 09JCZDJC25400 and No. 033605511).
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Cao, C., Sun, S., Wang, X. et al. Effects of manganese on the growth, photosystem II and SOD activity of the dinoflagellate Amphidinium sp.. J Appl Phycol 23, 1039–1043 (2011). https://doi.org/10.1007/s10811-010-9637-0
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10811-010-9637-0