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Evaluation of an improved strain of Porphyra yezoensis Ueda (Bangiales, Rhodophyta) with high-temperature tolerance

Abstract

The red algae Porphyra yezoensis has high commercial and nutritional value; however, its cultivation and product quality are jeopardized by global warming. Screening of high-temperature-tolerant strains would greatly reduce cultivation risks and benefit the Porphyra industry. Single somatic cells isolated enzymatically from the wild-type (WT) blades irradiated by 60Co-γ rays were screened at 25°C; one strain, T-17, displayed significant high-temperature resistance. Further culture studies showed that conchospore germlings of T-17 showed 76.2% survival at 23°C and 65.7% survival at 24°C, while the WT conchospore germlings survived at significantly lower percentages of 16.9% and 11.5%, respectively, over a period of 16 days. Furthermore, T-17 conchospore germlings divided at higher percentages of 100% at both 23 and 24°C compared to the WT conchospores with 99.8% and 81.8%, respectively. When the 50-day-old F1 gametophytic blades were transferred from the optimal temperature of 18°C to high temperatures of 23 and 24°C, the T-17 blades sustained growth over a 45-day period without rot, and their mean lengths increased by 20 and 4.2 times, respectively. The mean lengths of WT blades only increased by a factor of 0.6 and 0.4 times and were severely decayed after being cultured for 15 days at high temperatures. The mean wet weights of T-17 blades increased by 418.9 and 195.3 times, but only 7.8 and 4.6 times in WT at 23 and 24°C, respectively. These results indicate that T-17 is superior to WT in growth and high-temperature tolerance, which may offer a new cultivar for the nori industry as a high-temperature-resistant strain to counteract the effects of global warming.

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Acknowledgements

The study was supported in part by the National High Technology Research and Development Program of China (“863” Program) (grant no. 2006AA10A413), the National Natural Science Foundation of China (grant no. 30571443), Science and Technology Commission of Shanghai Municipality (grant no. 07XD14028), Shanghai Municipal Education Commission (project no. J50701), and the National Agriculture Public Welfare Foundation of China (grant no. 200903030).

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Correspondence to Xing-hong Yan.

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Zhang, Bl., Yan, Xh. & Huang, Lb. Evaluation of an improved strain of Porphyra yezoensis Ueda (Bangiales, Rhodophyta) with high-temperature tolerance. J Appl Phycol 23, 841–847 (2011). https://doi.org/10.1007/s10811-010-9587-6

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  • DOI: https://doi.org/10.1007/s10811-010-9587-6

Keywords

  • Conchospore
  • Gametophytic blade
  • Growth
  • High-temperature tolerance
  • Strain
  • Porphyra yezoensis