Journal of Applied Phycology

, Volume 23, Issue 4, pp 721–726 | Cite as

Protein, fatty acid, and pigment content of Chlorella vulgaris under different light regimes

  • Jafar SeyfabadiEmail author
  • Zohreh Ramezanpour
  • Zahra Amini Khoeyi


The effects of irradiance and photoperiod on growth rates, chlorophyll a, β-carotene, total protein, and fatty acid content of Chlorella vulgaris were determined. The maximum growth rate (1.13 day−1) was at 100 μmol photons m−2 s−1 and 16:8-h light/dark photoperiod. Chlorophyll a and β-carotene contents significantly differed under different light regimes with chlorophyll a content lower at high irradiance and longer light duration, while β-carotene showed the inverse trend. The total protein and fatty acid content also significantly differed in different light regimes; the maximum percentage of protein (46%) was at 100 μmol photons m−2 s−1 and 16:8 h photoperiod, and minimum (33%) was at 37.5 μmol photons m−2 s−1 and 8:16 h photoperiod; the total saturated fatty acids increased, while monounsaturated and polyunsaturated fatty acids decreased with increasing irradiance and light duration.


Chlorella vulgaris Light regime Growth Protein Total fatty acids Chlorophyll a β-carotene 



The authors are grateful to the manager and staff of International Sturgeon Research Institute (Gilan Province) who kindly cooperated with us throughout the study, and Tarbiat Modares University for the financial support.


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Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Jafar Seyfabadi
    • 1
    Email author
  • Zohreh Ramezanpour
    • 2
  • Zahra Amini Khoeyi
    • 1
  1. 1.Department of Marine Biology, Faculty of Natural Resources and Marine SciencesTarbiat Modares UniversityNoorIran
  2. 2.International Sturgeon Research InstituteGilanIran

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