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Purification of phycoerythrin from Porphyra yezoensis Ueda (Bangiales, Rhodophyta) using expanded bed absorption

Abstract

R-phycoerythrin was purified by means of phenyl-sepharose expanded bed absorption and DEAE-sepharose ion-exchange chromatography from Porphyra yezoensis, one of the largest and important aquaculture species in China. Final R-phycoerythrin preparation was characterized by purity ratio above 4 and the homogeneity in native PAGE, respectively. The results of absorption spectrum, fluorescence spectrum and SDS-PAGE were in agreement with previous reports on R-PE. The yield of R-phycoerythrin was 0.82 mg g−1 wet leafy gametophyte of P. yezoensis. This method is a high-protein recovery technology while reducing processing time, and is suitable for the large batch production of R-phycoerythrin, which will enhance the value of P. yezoensis in China, especially the inferior P. yezoensis which can not be used for flake processing.

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Acknowledgments

The work was supported by the 863 Project of China (No. 2007AA09Z406), the grant of National Natural Science Foundation of China (Nos. 40806063, 30830015) and the Project for Supporting the National Development (No. 2006BAD09A04).

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Correspondence to Guang-Ce Wang.

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Niu, JF., Chen, ZF., Wang, GC. et al. Purification of phycoerythrin from Porphyra yezoensis Ueda (Bangiales, Rhodophyta) using expanded bed absorption. J Appl Phycol 22, 25–31 (2010). https://doi.org/10.1007/s10811-009-9420-2

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  • DOI: https://doi.org/10.1007/s10811-009-9420-2

Keywords

  • Porphyra yezoensis
  • R-phycoerythrin
  • Hydrophobic interaction chromatography
  • Expanded bed absorption
  • Ion-exchange chromatography
  • Streamline column