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Journal of Applied Phycology

, 21:691 | Cite as

A molecular method for identification of the morphologically plastic invasive algal genera Eucheuma and Kappaphycus (Rhodophyta, Gigartinales) in Hawaii

  • Kimberly Y. Conklin
  • Akira Kurihara
  • Alison R. Sherwood
Article

Abstract

A paucity of diagnostic morphological characters for identification and high morphological plasticity within the genera Eucheuma and Kappaphycus has led to confusion about the distributions and spread of three introduced eucheumoid species in Hawaii. Entities previously identified as E. denticulatum, K. alvarezii, and K. striatum have had profound negative effects on Oahu’s coral reef ecosystems. The use of molecular tools to aid identification of algal species has been promising in other morphologically challenging taxa. We used three molecular markers (partial nuclear 28S rRNA, partial plastid 23S rRNA, and mitochondrial 5′ COI) and followed a DNA barcoding-like approach to identify Eucheuma and Kappaphycus samples from Hawaii. Neighbor-joining analyses were congruent in their separation of Eucheuma and Kappaphycus, and the resulting clusters were consistent with those revealed for global comparisons with the mitochondrial cox2-3 spacer and GenBank data. Based on these results, new insights were revealed into the distribution of these groups in Hawaii.

Keywords

28S rRNA 23S rRNA COI LSU UPA 

Notes

Acknowledgements

We are grateful to Brian Hauk and the State of Hawaii Department of Land and Natural Resources, Division of Aquatic Resources (DAR) for providing many of our samples. We would also like to thank Dr. Eric Conklin for field assistance and insights, Dr. Celia Smith for constructive criticism of the manuscript, and the Sherwood laboratory for general support. We also thank the staff of the Bernice P. Bishop Museum (Honolulu), especially Napua Narbottle, for their assistance and overall support of molecular analyses of archived material. This study was supported by the Hawaii Division of Aquatic Resources, a grant from the U.S. National Science Foundation to A.R.S. and G.G. Presting (DEB-0542608), and the University of Hawaii.

References

  1. Abbott IA (1996) Ethnobotany of seaweeds: clues to uses of seaweeds. Hydrobiologia 326/327:15–20CrossRefGoogle Scholar
  2. Abbott IA (1999) Marine red algae of the Hawaiian Islands. Bishop Museum Press, Honolulu, HawaiiGoogle Scholar
  3. Aguilan JT, Broom JE, Hemmingson JA, Dayrit FM, Montaño MNE, Dancel MCA, Niñonuevo MR, Furneaux RH (2003) Strutural analysis of carrageenan from farmed varieties of Philippine seaweed. Bot Mar 46:179–192CrossRefGoogle Scholar
  4. Bixler HJ (1996) Recent developments in manufacturing and marketing carrageenan. Hydrobiologia 326/327:35–57CrossRefGoogle Scholar
  5. Bulboa CR, de Paula EJ (2005) Introduction of non-native species of Kappaphycus (Rhodophyta, Gigartinales) in subtropical waters: comparative analysis of growth rates of Kappaphycus alvarezii and Kappaphycus striatum in vitro and in the sea in south-eastern Brazil. Phycol Res 53:183–188CrossRefGoogle Scholar
  6. Byrne K, Zuccarello GC, West J, Liao M-L, Kraft GT (2002) Gracilaria species (Gracilariaceae, Rhodophyta) from southeastern Australia, including a new species, Gracliaria perplexa sp. no.: morphology, molecular relationships and agar content. Phycol Res 50:295–311CrossRefGoogle Scholar
  7. Chandrasekaran S, Nagendra NA, Pandiaraja D, Krishnankutty N, Kamalakannan B (2008) Bioinvasion of Kappaphycus alvarezii on corals in the Gulf of Mannar, India. Curr Sci (Bangalore) 94:1167–1172Google Scholar
  8. Conklin EJ, Smith JE (2005) Abundance and spread of the invasive red algae, Kappaphycus spp., in Kaneohe Bay, Hawaii and an experimental assessment of management options. Biol Invasions 7:1029–1039CrossRefGoogle Scholar
  9. Dellaporta SL, Wood J, Hicks JB (1983) A plant DNA mini-preparations: version II. Plant Mol Biol Rep 1:19–21CrossRefGoogle Scholar
  10. Doty MS (1973) Farming the red seaweed, Eucheuma, for carrageenans. Micronesica 9:59–73Google Scholar
  11. Fredericq S, Freshwater DW, Hommersand MH (1999) Observations on the phylogenetic systematics and biogeography of the Solieriaceae (Gigartinales, Rhodophyta) inferred from rbcL sequences and morphological evidence. Hydrobiologia 398/399:25–38CrossRefGoogle Scholar
  12. Garguilo GM, Morabito M, Genovese G, De Masi F (2006) Molecular systematics and phylogenetics of Gracilariacean species from the Mediterranean Sea. J Appl Phycol 18:497–504CrossRefGoogle Scholar
  13. Glenn EP, Doty MS (1981) Photosynthesis and respiration of the tropical red seaweeds, Eucheuma striatum (Tambalang and Elkhorn varieties), and E. denticulatum. Aquat Bot 10:353–364CrossRefGoogle Scholar
  14. Glenn EP, Doty MS (1990) Growth of the seaweeds Kappaphycus alvarezii, K. striatum, and Eucheuma denticulatum as affected by environment in Hawaii. Aquaculture 84:245–255CrossRefGoogle Scholar
  15. Guillemin M-L, Ait Akki S, Givernaud T, Mouradi A, Valero M, Destombe C (2008) Molecular characterisation and development of rapid molecular methods to identify species of Gracilariaceae from the Atlantic coast of Morocco. Aquat Bot 89:324–330CrossRefGoogle Scholar
  16. Hebert PDN, Cywinska A, Ball SL, deWaard JR (2003) Biological identifications through DNA barcodes. Proc R Soc Lond B 270:313–321CrossRefGoogle Scholar
  17. Hughey JR, Silva PC, Hommersand M (2001) Solving taxonomic and nomenclatural problems in Pacific Gigartinaceae (Rhodophyta) using DNA from type material. J Phycol 37:1091–1109CrossRefGoogle Scholar
  18. Hurtado AQ, Agbayani RF (2002) Deep-sea farming of Kappaphycus using the multiple raft, long-line method. Bot Mar 45:438–444CrossRefGoogle Scholar
  19. Hurtado AQ, Critchley AT, Trespoey A, Bleicher-Lhonneur G (2008) Growth and carrageenan quality of Kappaphycus striatum var. sacol grown at different stocking densities, duration of culture and depth. J Appl Phycol 20:551–555CrossRefGoogle Scholar
  20. Kimura M (1980) A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120CrossRefPubMedGoogle Scholar
  21. Kooistra WHCF, Verbruggen H (2005) Genetic patterns in the calcified tropical seaweeds Halimeda opuntia, H. distorta, H. hederacea, and H. minima (Bryopsidales, Chlorophyta) provide insights in species boundaries and interoceanic dispersal. J Phycol 41:177–187CrossRefGoogle Scholar
  22. Llana EG (1991) Production and utilisation of seaweeds in the Philippines. INFOFISH International 1/91:12–17Google Scholar
  23. Lluisma AO, Ragan MA (1995) Relationships among Eucheuma denticulatum, Eucheuma isiforme, and Kappaphycus alvarezii (Gigartinales, Rhodophyta) based on nuclear ssu-rRNA gene sequences. J Appl Phycol 7:471–477CrossRefGoogle Scholar
  24. McHugh DJ (2003) A guide to the seaweed industry. Technical Report 441. Food and Agriculture Organization of the United Nations, Rome, ItalyGoogle Scholar
  25. Qiu B, Koh DA, Lumpkin C, Flament P (1997) Existence and formation mechanism of the North Hawaiian ridge current. J Phys Oceanog 27:431–444CrossRefGoogle Scholar
  26. Robba L, Russell SJ, Barker GL, Brodie J (2006) Assessing the use of the mitochondrial cox1 marker for use in DNA barcoding of red algae (Rhodophyta). Am J Bot 93:1101–1108CrossRefGoogle Scholar
  27. Rodgers SK, Cox EF (1999) Rate of spread of introduced rhodophytes Kappaphycus alvarezii, Kappaphycus striatum, and Gracilaria salicornia and their current distributions in Kaneohe Bay, Oahu, Hawaii. Pac Sci 53:232–241Google Scholar
  28. Russell DJ (1983) Ecology of the imported red seaweed Eucheuma striatum Schmitz on Coconut Island, Oahu, Hawaii. Pac Sci 37:87–107Google Scholar
  29. Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425PubMedGoogle Scholar
  30. Saunders GW (2005) Applying DNA barcoding to red macroalgae: a preliminary appraisal holds promise for future applications. Philos Trans R Soc Lond B 360:1879–1888CrossRefGoogle Scholar
  31. Sherwood AR, Presting GG (2007) Universal primers amplify a 23S rDNA plastid marker in eukaryotic algae and cyanobacteria. J Phycol 43:605–608CrossRefGoogle Scholar
  32. Sherwood AR, Vis ML, Entwisle TJ, Necchi O Jr., Presting GG (2008) Contrasting intra versus interspecies DNA sequence variation for representatives of the Batrachospermales (Rhodophyta): insights from a DNA barcoding approach. Phycol Res 56:269–279CrossRefGoogle Scholar
  33. Smith JE, Hunter CL, Smith CM (2002) Distribution and reproductive characteristics of nonindigenous and invasive marine algae in the Hawaiian Islands. Pac Sci 56:299–315CrossRefGoogle Scholar
  34. Tamura K, Dudley J, Nei M, Kumar S (2007) MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol Biol Evol 24:1596–1599CrossRefPubMedGoogle Scholar
  35. Thompson JD, Higgins DG, Gibson TJ (1994) CLUSTAL W: improving the sensitivity of progressive multiple sequence alignments through sequence weighting, position specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680CrossRefPubMedGoogle Scholar
  36. Villanueva RD, Montaño MNE, Romero JB (2008) Iota-carrageenan from a newly farmed, rare variety of eucheumoid seaweed–"endong". J Appl Phycol doi: 10.1007/s10811-008-9356-y
  37. Woo MML (2000) Ecological impacts and interactions of the introduced red alga, Kappaphycus striatum, in Kaneohe Bay, Oahu. MSc Thesis. Honolulu: University of HawaiiGoogle Scholar
  38. Zuccarello GC, Critchley AT, Smith JE, Sieber V, Bleicher-Lhonneur G (2006) Systematics and genetic variation in commercial Kappaphycus and Eucheuma (Solieriaceae, Rhodophyta). J Appl Phycol 18:643–651CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • Kimberly Y. Conklin
    • 1
  • Akira Kurihara
    • 1
  • Alison R. Sherwood
    • 1
  1. 1.Botany DepartmentUniversity of HawaiiHonoluluUSA

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