Journal of Applied Phycology

, Volume 21, Issue 3, pp 315–319 | Cite as

Using chlorophyll fluorescence to monitor yields of microalgal production

  • Mitsuko Obata
  • Tatsuki Toda
  • Satoru TaguchiEmail author


A monitoring system for microalgal production was developed over a 12:12-h light:dark cycle at a steady state of growth to test the feasibility of estimating carbon production using the fluorescence method. An empirical linear relationship between the electron transport rate, based on the fluorescence method, and carbon assimilation, based on the conventional carbon method, was successfully obtained. The results demonstrate the relevance of the electron transport rate in determining carbon production of microalgae under steady-state growth conditions.


Carbon assimilation rate Chlorella vulgaris Electron transport rate PSII operating efficiency 



Technical assistance was provided by H. Tomii. This study was partially supported by the University-Industry Joint Research project for private universities and a matching fund subsidy from the Ministry of Education, Culture, Sports, Science and Technology #2004–4.


  1. Ahn CY, Chung AS, Oh HM (2002) Diel rhythm of algal phosphate uptake rates in P-limited cyclostats and simulations of its effect on growth and competition. J Phycol 38:695–704 doi: 10.1046/j.1529-8817.2002.01232.x CrossRefGoogle Scholar
  2. Borowitzka MA (2005) Culturing microalgae in outdoor ponds. In: Andersen RA (ed) Algal culturing techniques. Elsevier, Amsterdam, pp 205–218CrossRefGoogle Scholar
  3. Dauta A, Devaux J, Piquemal F, Bouminich L (1999) Growth rate of four freshwater algae in relation to light and temperature. Hydrobiology 207:221–226CrossRefGoogle Scholar
  4. Dubinsky Z, Falkowski PG, Wyman K (1986) Light harvesting and utilization by phytoplankton. Plant Cell Physiol 27:1335–1349Google Scholar
  5. Eppley RW (1981) Relations between nutrient assimilation and growth in phytoplankton with a brief review of estimates of growth rate in the ocean. Can Bull Fish Aquat Sci 210:251–263Google Scholar
  6. Genty B, Briantais J-M, Baker NR (1989) The relationship between the quantum yield of photosynthetic electron transport and quenching of chlorophyll fluorescence. Biochim Biophys Acta 990:87–92Google Scholar
  7. Hama T, Miyazaki T, Ogawa Y, Iwakuma T, Takahashi M, Ichimura S (1983) Measurement of photosynthetic production of a marine phytoplankton population using a stable 13C isotope. Mar Biol 73:31–36 doi: 10.1007/BF00396282 CrossRefGoogle Scholar
  8. Hartig P, Wolfstein K, Lippemeier S, Colijn F (1998) Photosynthetic activity of natural microphytobenthos populations measured by fluorescence (PAM) and 14C-tracer methods: in comparison. Mar Ecol Prog Ser 166:53–62 doi: 10.3354/meps166053 CrossRefGoogle Scholar
  9. Head EJH, Horne EPW (1993) Pigment transformation and vertical flux in an area of convergence in the North Atlantic. Deep Sea Res Part II Top Stud Oceanogr 40:329–346 doi: 10.1016/0967-0645(93)90020-N CrossRefGoogle Scholar
  10. Hu Q, Guterman H, Richmond A (1996) A flat inclined modular photobioreactor (FIMP) for outdoor mass cultivation of photoautotrophs. Biotechnol Bioeng 51:51–60 doi: 10.1002/(SICI)1097-0290(19960705)51:1<51::AID-BIT6>3.0.CO;2-# PubMedCrossRefGoogle Scholar
  11. Iwamoto H (2006) Industrial production of microalgal cell-mass and secondary products-major industrial species Chlorella. In: Richmond A (ed) Handbook of microalgal culture. Blackwell, Oxford, pp 255–263Google Scholar
  12. Keiding N, Rudolph N, Moller U (1984) Diurnal variation in influx and transition intensities in the S phase of hamster cheek epithelium cells. In: Edmunds L Jr (ed) Cell cycle clocks. Dekker, New York, pp 135–159Google Scholar
  13. Kirk JTO (1994) Light and photosynthesis in aquatic ecosystem. Cambridge University Press, CambridgeGoogle Scholar
  14. Leonardos N (2008) Physiological steady state of phytoplankton in the field? An example based on pigment profile of Emiliania huxlei (Haptophyta) during a light shift. Limnol Oceanogr 53:306–311Google Scholar
  15. Lewis MR, Smith JC (1983) A small volume, short-incubation-time method for measurement of photosynthesis as a function of incident irradiance. Mar Ecol Prog Ser 13:99–102 doi: 10.3354/meps013099 CrossRefGoogle Scholar
  16. Lippemeier S, Hintze R, Vanselow KH, Hartig P, Colijn F (2001) In-line recording of PAM fluorescence of phytoplankton culture as a new tool for studying effect of fluctuating nutrient supply on photosynthesis. Eur J Phycol 36:89–100 doi: 10.1080/09670260110001735238 CrossRefGoogle Scholar
  17. MacIntyre HL, Cullen JJ (2005) Using cultures to investigate the physiological ecology of microalgae. In: Andersen RA (ed) Algal culturing techniques. Elsevier, Amsterdam, pp 287–326CrossRefGoogle Scholar
  18. Markl H (1980) Modeling of algal production systems. In: Shelef G, Soeder CJ (eds) Algal biomass. Elsevier, Amsterdam, pp 363–383Google Scholar
  19. Nagao N, Toda T, Takahashi K, Hamasaki K, Taguchi S (2001) High ash content in net-plankton samples from shallow coastal water. Possible source of error in dry weight measurement of zooplankton biomass. J Oceanogr 57:105–107 doi: 10.1023/A:1016050728836 CrossRefGoogle Scholar
  20. Schreiber U, Bilger W, Neubauer C (1994) Chlorophyll fluorescence as a non-intrusive indicator for rapid assessment of in vivo photosynthesis. Ecol Stud 100:49–70Google Scholar
  21. Schreiber U, Bilger W, Hormann H, Neubauer C (1998) Chlorophyll fluorescence as a diagnostic tool: basics and some aspects of practical relevance. In: Raghavendra AS (ed) Photosynthesis: a comprehensive treatise. Cambridge University Press, pp 320–336Google Scholar
  22. Schuter B (1979) A model of physiological adaptation in unicellular algae. J Theor Biol 78:519–552 doi: 10.1016/0022-5193(79)90189-9 CrossRefGoogle Scholar
  23. Suzuki R, Ishimaru T (1990) An improved method for the determination of phytoplankton chlorophyll using N,N-dimethylformamide. J Oceanogr 46:190–194Google Scholar
  24. Torzillo G, Bernadini P, Masojidek J (1998) On-line monitoring of chlorophyll fluorescence to assess the extent of photoinhibition of photosynthesis induced by high oxygen concentration and low temperature and its effects on the productivity of outdoor cultures of Spirulina platensis (Cyanobacteria). J Phycol 34:504–510 doi: 10.1046/j.1529-8817.1998.340504.x CrossRefGoogle Scholar
  25. Watanabe A (1960) List of algal strains in collection at the Institute of Applied Microbiology, University of Tokyo. J Gen Appl Microbiol 6:283–292 doi: 10.2323/jgam.6.283 CrossRefGoogle Scholar
  26. Wood AM, Everroad RC, Wingard LM (2005) Measuring growth rates in microalgal cultures. In: Andersen RA (ed) Algal culturing techniques. Elsevier, Amsterdam, pp 269–285Google Scholar

Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  1. 1.Department of Environmental Engineering for Symbiosis, Faculty of EngineeringSoka UniversityTokyoJapan

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