Vanadium-dependent bromoperoxidases from Gracilaria algae

Abstract

Red algae from the Gulf of Thailand were examined for haloperoxidatic activity. Six species, Gracilaria changii, G. edulis, G. firma, G. fisheri, G. salicornia, and G. tenuistipitata, showed bromoperoxidatic activity. Duplicate polyacrylamide electrophoretic gels showed enzyme activity patterns developed by phenol red staining for bromoperoxidatic activity and by 3,3′-diaminobenzidine staining for peroxidatic activity. All algae gave isoenzymic bromoperoxidatic activity bands and peroxidatic activity bands, but there were peroxidatic and bromoperoxidatic activity bands that did not correspond. The bromoperoxidatic activity of the crude enzyme extracts as well as previously dialyzed enzyme solutions was enhanced significantly by incubation with vanadium pentoxide. The three purified bromoperoxidases from G. fisheri contained vanadium, and their relative activities corresponded to the ratio of vanadium to enzyme. In addition, they were not inhibited by H2O2. These data confirm that the enzymes are vanadium bromoperoxidases.

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Fig. 5a–c

Abbreviations

Apo-BPO:

Apo-bromoperoxidase

BPO:

Bromoperoxidase

DAB:

3,3′-Diaminobenzidine

HPO:

Haloperoxidase

GfL:

Low-molecular-weight bromoperoxidase from G. fisheri

GfH:

High-molecular-weight bromoperoxidase from G. fisheri

MCD:

Monochlorodimedone

U:

Unit of enzyme activity (μmole MCD decrease.min−1)

V-Apo:

Vanadium-reactivated apo-bromoperoxidase

V-BPO:

Vanadium bromoperoxidase

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Acknowledgements

This work was supported by a grant from the National Research Council of Thailand. We thank Prof. Dr. Khanjanapaj Lewmanomont Faculty of Fisheries, Kasetsart University, for her assistance in identification of algal samples.

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Correspondence to Tuangporn Suthiphongchai.

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Suthiphongchai, T., Boonsiri, P. & Panijpan, B. Vanadium-dependent bromoperoxidases from Gracilaria algae. J Appl Phycol 20, 271–278 (2008). https://doi.org/10.1007/s10811-007-9243-y

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Keywords

  • Bromoperoxidase staining
  • Red algae
  • V-BPO