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Sulfated polysaccharide purified from Ecklonia cava accelerates antithrombin III-mediated plasma proteinase inhibition

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Abstract

Surface plasmon resonance is an important technique for studying molecular interactions and was used to investigate the molecular interaction of anticoagulant sulfated polysaccharides purified from an enzymatic hydrolysate of the brown alga Ecklonia cava (ECA) with blood coagulation factors. In a direct binding assay, binding affinity between ECA/antithrombin III (ATIII) and activated blood coagulation factors was in the order: factor VIIa (FVIIa) > factor Xa (FXa) > thrombin (FIIa); kinetic analysis determined K D values of ECA for FVIIa, FXa, and FIIa of 15.1, 45.0 and 65.0 nM, respectively. Therefore, ECA strongly and selectively (FVII, FX, and FII) enhanced ATIII-mediated coagulation factor inhibition in both the extrinsic and common coagulation pathways. This may contribute to its high anticoagulant activity in vitro. The low cytotoxicity of ECA to venous endothelial cell line (ECV-304) also expands its value in future in vivo studies. However, to utilize it as a model for novel anticoagulant agents, its possible interference with other anticoagulant mechanisms must be addressed.

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Acknowledgements

This research was supported by a grant (p-2004-03) from the Marine Bioprocess Research Center of the Marine Bio 21 Center funded by the Ministry of Maritime Affairs and Fisheries, Republic of Korea. This work was also supported by the Korea Research Foundation Grant (KRF-2004-908-F00005), Republic of Korea.

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  1. Won-Kyo Jung

    Present address: Department of NOAA Sea Grant Development and Food Science, Louisiana State University, Baton Rouge, Louisiana, 70803, USA

Authors and Affiliations

  1. Department of Chemistry, Pukyong National University, Busan, 608-737, South Korea

    Won-Kyo Jung & Se-Kwon Kim

  2. Faculty of Applied Marine Science, Cheju National University, Jeju, 690-756, South Korea

    Yasantha Athukorala, Seon Heui Cha, Kwang-Sik Choi & You-Jin Jeon

  3. Department of Veterinary Science, Cheju National University, Jeju, 690-756, South Korea

    Young-Jae Lee

  4. Department of Food Science and Biotechnology of Animal Resources, Kunkuk University, Seoul, 111-221, South Korea

    Chi-Ho Lee

  5. Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, AB, T6G 2P5, Canada

    Thava Vasanthan

  6. Department of Food Science and Technology, Sejong University, 98 Gunja-dong, Gwangjin-gu, Seoul, 143-747, South Korea

    Sang-Ho Yoo

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  1. Won-Kyo Jung
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  2. Yasantha Athukorala
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Correspondence to You-Jin Jeon.

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Jung, WK., Athukorala, Y., Lee, YJ. et al. Sulfated polysaccharide purified from Ecklonia cava accelerates antithrombin III-mediated plasma proteinase inhibition. J Appl Phycol 19, 425–430 (2007). https://doi.org/10.1007/s10811-006-9149-0

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  • DOI: https://doi.org/10.1007/s10811-006-9149-0

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