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Trait evaluation and trial cultivation of Dongfang No. 2, the hybrid of a male gametophyte clone of Laminaria longissima (Laminariales, Phaeophyta) and a female one of L. japonica

Journal of Applied Phycology volume 19, pages 139–151 (2007)Cite this article

Abstract

Direct cultivation of the first filial generation of gametophyte clones from different Laminaria species is a highly effective way of utilizing kelp heterozygous vigor (heterosis). A male gametophyte clone of L. longissima Miyabe and a female one of L. japonica Areschoug were hybridized, generating Dongfang No. 2 hybrid kelp. This hybrid kelp was used directly in trial cultivation, and its agronomical traits were evaluated. L. longissima and L. japonica are obviously different and complement each other in their morphological characteristics and ecological performances. The hybrid of their gametophyte clones, Dongfang No. 2, showed 56.8% heterozygous vigor in yield. It also showed increased yields of 41.0 and 76.4% compared to the widely used commercial kelps Variety 1 and Variety 2, respectively. In large-scale cultivation trials at different locations and in different years, Dongfang No. 2 attained significantly higher yields than Varieties 1 and 2, increasing yield by 26.4% on average over Variety 1 and by 65.0% over the other. Dongfang No. 2 has a robust holdfast and a wide, long and deep-brown uniform blade, which shows a distinct middle groove. In addition to yield, Dongfang No. 2 also demonstrates obvious heterozygous vigor in other agronomic traits. It is resistant to strong irradiance, as the two commercial varieties are, has an appropriate vegetative maturation time, and adapts well to a range of different culture conditions. The parentage analysis using AFLP of total DNA and SNP of the ITS region of ribosomal RNA transcription unit showed that Dongfang No. 2 is the real hybrid of L. japonica and L. longissima.

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References

  • Algal Genetic Research and Breeding Team of Oceanography Institute of Chinese Academy of Science and Algal Culturing Team of Qingdao Maricultural Institute (1976) Breeding of a novel variety with high yield potential and iodine content (in Chinese with English abstract). Sci China 5:512–517

    Google Scholar 

  • Aguilar JF, Rossello JA, Feliner GN (1999) Nuclear ribosomal DNA (nrDNA) concerted evolution in natural and artificial hybrids of Armeria (Plumbaginaceae). Mol Biol 8:1341–1346

    Google Scholar 

  • Fang TC, Wu CY, Jiang Y, Li TT, Ren GZ (1962) The breeding of a new breed of Haidai (Laminaria japonica Aresch.) and its preliminary genetic analysis (in Chinese with English abstract). Acta Bot Sinica 10(3):197–209

    Google Scholar 

  • Fang Z, Ou Y, Cui J, Dai J (1978) Success in culturing clones of the gametophytes of Laminaria japonica (in Chinese with English abstract). Transactions of Science 2:115–116

    Google Scholar 

  • Fang Z, Ou Y, Cui J (1983) Experiments on heterosis of Laminaria (in Chinese with English abstract). Mar Sci Bull (China) 2(6):57–60

    Google Scholar 

  • Fang Z, Ou Y, Cui J (1985) Breeding of hybrid Laminaria “Danza No.10”: An application of the Laminarian haploid cell clones (in Chinese with English abstract). J Shangdong Coll Oceanol 15(1):64–72

    Google Scholar 

  • Ganley ARD, Scott B (2002) Concerted evolution in the ribosomal RNA genes of an Epichloe endophyte hybrid: Comparison between tandemly arranged rDNA and dispersed 5S rRNA genes. Fung Genet Biol 35:39–51

    Article  CAS  Google Scholar 

  • Li HJ (1990) Notes on the Laminaria raft cultivation method (in Chinese). Mariculture 1990(1/2):41–48

    Google Scholar 

  • Li D, Zhou Z, Liu H, Wu C (1999) A new method of Laminaria japonica strain selection and sporeling raising by the use of gametophyte clones. Hydrobiologia 398/399:473–476

    Article  Google Scholar 

  • Li D, Lu Y, Wu C (2002) History and current status of kelp breeding and sporeling raising technology (in Chinese). Biol Bull 37(8):1–3

    Google Scholar 

  • Li D, Wu C, Liu W, Zang K, Xia J (2003a) A new technology of Laminaria japonica sporeling culture by the use of gametophyte clones (in Chinese with English abstract). Acta Oceanol Sinica 25(5):141–145

    CAS  Google Scholar 

  • Li M, Yu B, Hu W, Chu C, Xie H, Cui Y (2003b) Study on the large scale raising technique of Laminaria japonica sporelings (in Chinese). Shandong Fisheries 20(9):25–27

    CAS  Google Scholar 

  • Li Z, Zhang Q, Yang Y, Xie J, Luo S (2003c) Study on the technique of large-scale culture of the clone of Laminaria gametophyte (in Chinese). Shandong Fisheries 20(5):1–3

    Google Scholar 

  • Nei M, Li WH (1979) Mathematical model for studying genetic variation for restriction endonuclease. Proc Natl Acad Sci USA 76:5269–5273

    PubMed  Article  CAS  Google Scholar 

  • Ou Y, Cui J, Fang Z (1983) Experiment of Laminaria heterosis (in Chinese with English abstract). Mar Sci Bull 2(6):57–61

    Google Scholar 

  • Patwary MU, Ron MM (1993) Revealing genetic markers on Gelidium vagum (Rhodophyta) through the random amplified polymorphic DNA (RAPD) technique. J Phycol 19:216–222

    Article  Google Scholar 

  • Pikaard CS (1999) Nucleolar dominance and silencing of transcription. Trends Plant Sci 4(12):478–483

    Google Scholar 

  • Pikaard CS (2000) The epigenetics of nucleolar dominance. TIG 16(11):495–500

    PubMed  CAS  Google Scholar 

  • Schlotterer C, Tautz D (1994) Chromosomal homogeneity of Drosophila ribosomal DNA arrays suggests intrachromosomal exchanges drive concerted evolution. Curr Biol 4:777–783

    PubMed  Article  CAS  Google Scholar 

  • Scoggan S, Zhuang Z, Wang F (1989) Laminaria Seafarming in China: training manual 89/5 (RAS/86/024). Qingdao, China

  • Tian Z, Yuan B (1989) Report of the breeding of a novel kelp variety, “Zaohoucheng No.1” (in Chinese). Mariculture 1:7–17

    Google Scholar 

  • Tseng CS (2001) Algal biotechnology industries and research activities in China. J Appl Phycol 13:375–380

    Article  Google Scholar 

  • Tseng CK, Sun KY, Wu CY (1955) On the cultivation of Haidai (Laminaria japonica Aresch) by summering young sporophytes at low temperature (in Chinese with English abstract). Acta Bot Sinica 4(3):255–264

    Google Scholar 

  • Vos P, Hogers R, Bleeker M, Reijans M, van de Lee T, Homes M, Frijters A, Pot J, Peleman J, Kuiper M, Zabeau M (1995) AFLP: a new technique for DNA fingerprinting. Nucl Acids Res 23:4407–4414

    PubMed  Article  CAS  Google Scholar 

  • Wang S (1994) Seaweed biotechnology (in Chinese). Shanghai Scientific & Technical Publishers, Shanghai, China, pp. 104–111

    Google Scholar 

  • Yoon HS, Lee JY, Boo SM, Bhattacharya D (2001) Phylogeny of Alariaceae, Laminariaceae, and Lessoniaceae (Phaeophyceae) based on plastid-encoded RuBisCo spacer and nuclear-encoded ITS sequence comparison. Mol Phylogenet Evol 21:231–243

    PubMed  Article  CAS  Google Scholar 

  • Zemke-White WL, Ohno M (1999) World seaweed utilization: An end-of-century summary. J Appl Phycol 11:369–376

    Article  Google Scholar 

  • Zhang Z, Fan C, Cao S, You X (1998) Study on the indoor culture and cultivation of Laminaria longissima Mijabe. J Dalian Fish Univ 13(4):1–6

    CAS  Google Scholar 

  • Zhang Z, Fan C, Cao S, You X, Su Y (1999) Study on interspecific crossing of Laminaria species (in Chinese with English abstract). J Dalian Fish Univ 14(4):13–17

    CAS  Google Scholar 

  • Zhang Q, Qu S, Xie J, Liu J, Cui F (2000) Experiment of culturing Laminaria longissima in Zhifu Bay. Shandong Fisheries 17(1):10–11

    Google Scholar 

  • Zhang Q, Liu S, Qu S, Tian Z, Guo Z, Cong Y, Li Z (2001) Studies on rearing new variety of kelp “901” (in Chinese with English abstract). Trans Oceanol Limnol 2:46–53

    Google Scholar 

  • Zhou Z, Wu C (1998) Clone culture of Laminaria japonica and induction of its sporophytes (in Chinese with English abstract). Chinese J Biotechnol 14(1):109–111

    Google Scholar 

Download references

Acknowledgments

This work was supported by The Popularization Project of Agricultural Elite Varieties of Shandong Province (2004113) and National Natural Science Foundation of China (30471318). The authors appreciate the reviewers and editors for their kind and detailed corrections and constructive comments. We thank also editors and reviewers for their patience in processing and reviewing our manuscript and their encouragements. Without their endeavors, this paper would not have come out as it is.

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Authors and Affiliations

  1. Shandong Oriental Ocean Sci-tec Co., Ltd., Yantai, Shandong, 264003, People’s Republic of China

    Xiaojie Li, Yizhou Cong, Shancun Qu, Zhiling Li, Guowen Wang, Zhuangzhi Zhang, Shiju Luo, Hongliang Dai, Jianzu Xie, Guangliang Jiang & Jialiang Liu

  2. College of Marine Life Science, Ocean University of China, 5 Yushanlu Road, Qingdao, Shandong, 266003, People’s Republic of China

    Xiaojie Li, Guanpin Yang & Yuanyuan Shi

  3. Wendeng Fishery Technique Popularization Station of Shandong Province, Wendeng, Shandong, 264400, People’s Republic of China

    Tongyong Wang

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  1. Xiaojie Li
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  2. Yizhou Cong
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  3. Guanpin Yang
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  4. Yuanyuan Shi
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  9. Shiju Luo
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  11. Jianzu Xie
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  12. Guangliang Jiang
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  14. Tongyong Wang
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Corresponding author

Correspondence to Guanpin Yang.

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X. Li and Y. Cong contributed equally to this work.

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Li, X., Cong, Y., Yang, G. et al. Trait evaluation and trial cultivation of Dongfang No. 2, the hybrid of a male gametophyte clone of Laminaria longissima (Laminariales, Phaeophyta) and a female one of L. japonica . J Appl Phycol 19, 139–151 (2007). https://doi.org/10.1007/s10811-006-9120-0

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  • DOI: https://doi.org/10.1007/s10811-006-9120-0

Key words

  • Dongfang No. 2
  • hybrid kelp
  • gametophyte clone
  • heterosis
  • hybridization