Skip to main content

Effects of UVB Radiation on the Initial Stages of Growth of Gigartina Skottsbergii, Sarcothalia Crispata and Mazzaella Laminarioides (Gigartinales, Rhodophyta)

Abstract

The effects of UVB radiation on the growth of macroalgal thalli were evaluated using tetrasporophytic fronds of the Rhodophytes Gigartina skottsbergii, Sarcothalia crispata and Mazzaella laminarioides. The tetrasporophytic fronds were collected from nature and the tetrasporophyte sporelings grown in a temperature regulated chamber at 8 ± 2 C with a 12L:12D (Light: Dark) photoperiod, Photosynthetically Active Radiation (PAR) of 55 μmol photons m−2 s−1 and seawater enriched with 20 mL L−1 of Provasoli medium. We exposed the thalli of these macroalgae to PAR (55 μmol photons m−2 s−1) and three treatments using a combination of PAR with three different levels of UVB radiation (0.10, 0.15 and 0.23 W m−2 for G. skottsbergii and S. crispata and 0.02, 0.05 and 0.10 W m−2 for M. laminarioides) during a period of 71 days. Growth of thalli was quantified by measuring their length using digitized photographs of samples.

Important differences were detected in the growth of individuals cultured under the effects of UVB radiation, when compared to the control (i.e. plants exposed to PAR only). In the case of G. skottsbergii and S. crispata higher levels of UVB radiation resulted in slower growth of thalli. In nearly all measurements for the first two species, UVB radiation levels of 0.1 W m−2 induced differences in thallus growth, while for M. laminarioides levels of UVB radiation of 0.1 W m−2 were effective only after a prolonged period of exposure.

Differential effects of UVB radiation on G. skottsbergii, S. crispata and M. laminarioides could interfere with the natural populations of these economically important macroalgal species in southern Chile, where they occur under the annual influence of the Antarctic Ozone Hole and the general thinning of the ozone layer.

This is a preview of subscription content, access via your institution.

References

  • Anderson TW (1958) An Introduction to Multivariate Statistical Analysis. John Wiley & Sons, Inc, New York.

    Google Scholar 

  • Barnes P, Flint S, Cadwell M (1990) Morphological responses of crop and weed species of different growth forms to ultraviolet-B radiation. Am. J. Bot. 77: 1354--1360.

    Article  Google Scholar 

  • Bischof K, Hanelt D, Wiencke C (1998) UV-radiation can affect depth-zonation of Antarctic macroalgae. Mar. Biol. 131(4): 597--605.

    Article  Google Scholar 

  • Bischof K, Hanelt D, Wiencke C (2000) Effects of ultraviolet radiation on photosynthesis and related enzyme reactions of marine macroalgae. Planta 211: 555--562.

    PubMed  CAS  Article  Google Scholar 

  • Buma AGJ, van Oijen T, van de Poll W, Veldhuis MJW, Gieskes WWC (2000) The sensivity of Emiliana huxleyi (Prymnesiophyceae) to ultraviolet-B radiation. J. Phycol. 36: 296--303.

    CAS  Article  Google Scholar 

  • Buma AGJ, Van Hannen EJ, Roza L, Veldhuis MJW, Gieskes WWC (1995) Monitoring ultraviolet-B-induced DNA damage in individual diatom cells by immunofluorescent thymine dimer detection. J. Phycol. 31: 314--321.

    CAS  Article  Google Scholar 

  • Casiccia C, Kirchhoff VWJH, Torres A (2003) Simultaneous measurements of ozone and ultraviolet radiation: Spring 2000, Punta Arenas, Chile. Atmospheric Environment.

  • Cuadra P, Harborne J, Waterman P (1997) Increases in flavonoid and photosynthetic pigments in Gnaphalium luteo-album in response to UV-B radiation. Phytochemistry 45: 1377--1383.

    CAS  Google Scholar 

  • Diffey B (1991) Solar ultraviolet radiation effects on biological system. Physics in Medicine and Biology 36(3): 299--328.

    PubMed  CAS  Article  Google Scholar 

  • Döler G (1996) Effect of UV irradiance on utilization of inorganic nitrogen by the Antarctic diatom Odontella weissflogii (Janisch) Grunow. Botanica Acta 109: 35--42.

    Google Scholar 

  • Döler G, Hagmeier E, David C (1995) Effects of solar and artificial UV irradiation on pigments and assimilation of 15N ammonium and 15N nitrate by macroalgae. J. Photochem. Photobiol. 32: 107--113.

    Google Scholar 

  • Dring M, Makarov V, Schoschina E, Lorenz M, Lüning K (1996) Influence of ultraviolet-radiation on chlorophyll fluorescence and growth in different life-history stages of three species of Laminaria (Phaeophyta). Mar. Biol. 126(1): 183--191.

    CAS  Article  Google Scholar 

  • Figueroa FL (2002) Bio-optical characteristics of Gerlache and Bransfield Strait waters during an Antarctic summer cruise. Deep Sea Res. II 49: 675--691.

    CAS  Article  Google Scholar 

  • Figueroa FL, Escassi E, Pérez-Rodruíguez, Korbee N, Giles AD, Johnsen G (2003) Effects of short-term irradiation on photoinhibition and accumulation of mycosporine-like amino acids in sun and shade species of the red algae genus Porphyra. J. Photochem. Photobiol. B: Biol. 69: 21--30.

    CAS  Article  Google Scholar 

  • Franklin LA, Forster RM (1997) The changing irradiance environment: Consequences from marine macrophyte physiology, productivity and ecology. Eur. J. Phycol. 32: 207--232.

    Google Scholar 

  • Franklin LA, Yakovleva I, Karsten U, Lüning K (1999) Synthesis of mycosporine-like-amino acids in Chondrus crispus (Florideophyceae) and the consequences for sensitivity to ultraviolet B radiation. J. Phycol. 35: 682--693.

    CAS  Article  Google Scholar 

  • Franklin LA, Kräbs G, Kuhlenkamp R (2001) Blue light and UV-A radiation control the synthesis of mycosporine-like amino acids in Chondrus crispus (Florideophyceae). J. Phycol. 37: 257--270.

    CAS  Article  Google Scholar 

  • Grobe C, Murphy T (1994) Inhibition of growth of Ulva expansa (Cholorophyta) by ultraviolet-B radiation. J. Phycol. 30: 783--790.

    Article  Google Scholar 

  • Grobe C, Murphy T, (1997) Artificial ultraviolet-B radiation and cell expansion in the intertidal alga Ulva expansa (Setch.) S. & G. (Chlorophyta). J. Exp. Mar. Biol. Ecol. 217: 209--223.

    Article  Google Scholar 

  • Grobe C, Murphy T (1998) Solar ultraviolet-B radiation effects on growth and pigment composition of the intertidal alga Ulva expansa (Setch.) S. & G. (Chlorophyta). J. Exp. Mar. Biol. Ecol. 225: 39--51.

    CAS  Article  Google Scholar 

  • Hansen JE (1980) Physiological considerations in the mariculture of red algae. In Abbott IA, Foster MS, Ekund LF (eds), Pacific Seaweed Aquaculture. Publications of the California Sea Grant Program, La Jolla, pp. 80--91.

    Google Scholar 

  • Huovinen P, Gómez I, Figueroa FL, Ulloa N, Morales V, Lovengreen C (2004) Ultraviolet-absorbing micosporine-like amino acids in red macroalage from Chile. Botanica Marina 47: 21--29.

    CAS  Article  Google Scholar 

  • Karsten U, Sawall T, Wiencke C (1998). A survey of the distribution of UV-absorbing substances in tropical macroalgae. Physiol. Res. 46(4): 271--279.

    CAS  Google Scholar 

  • Karsten U, Bischof K, Hanelt D, Tüg H, Wiencke C (1999) The effects of ultraviolet radiation on photosynthesis and ultraviolet-absorbing substances in the endemic Arctic macroalga Devaleraea ramentacea (Rhodophyta). Physiol. Plant 105: 58--66.

    CAS  Article  Google Scholar 

  • Kirchhoff VWJH, Zamorano F, Casiccia C (1997) UVB enhancements at Punta Arenas, Chile. J. Photochem. Photobiol. B: Biol. 38: 174--177.

    CAS  Article  Google Scholar 

  • Kolli B, Tiwari S, Mohanty P (1998) Ultraviolet-B induced damage to photosystem II in intact filaments of Spirulina platensis. Zeitschrift feur Naturfoschung Section C 53(5--6): 369--377.

    CAS  Google Scholar 

  • Komhyr W, Grass R, Leonard R (1989) Total ozone, ozone vertical distributions, and stratospheric temperatures at South Pole, Antarctica, in 1996 and 1997. J. Geophys. Res. 94: 11429--11436.

    CAS  Google Scholar 

  • Kuhlenkamp R, Franklin LA, Lüning K (2001) Effect of solar UV radiation on growth in the marine macroalgae Dicyota dichotoma (Phaeophyceae) at Helgoland and its ecological consequences. Helgoland Mar. Res. 55: 77--86.

    Article  Google Scholar 

  • Larkum A, Wood W (1993). The effect of UVB radiation on photosynthesis and respiration of phytoplankton benthic macroalgae and seagrasses. Photosynth. Res. 36: 17--23.

    CAS  Article  Google Scholar 

  • Maegawa M, Kunieda M, Kida W (1993) The influence of ultraviolet radiation on the photosynthetic activity of several red algae from different depths. Jpn. J. Phycol. 41: 207--214.

    CAS  Google Scholar 

  • Makarov M (1999) Influence of ultraviolet radiation on the growth of the dominant macroalgae of the Barents Sea. Chemosphere: Global Change Science 1: 461--467.

    Article  Google Scholar 

  • Meindl U, Lütz C (1996) Effects of UV irradiation on cell development and ultrastructure of the green alga Micrasterias. J. Photochem. Photobiol. 36(3): 285--292.

    CAS  Article  Google Scholar 

  • Molina X, Montecino V (1996) Acclimation to UV irradiance in Gracilaria chilensis Bird, Mc Lachlan & Oliveira (Gracilariles, Rhodophyta). Hydrobiologia 326/327: 415--420.

    Article  Google Scholar 

  • Montecino V, Pizarro G (1995) Phytoplankton acclimation and spectral penetration of UV irradiance of the central Chilean coast. Mar. Ecol. Prog. Ser. 121: 1--3.

    Google Scholar 

  • Navarro NP (2004) Efeitos da radiačäo UVB em Iridaea cordata (Gigartinales, Rhodophyta). Dissertačäo apresentada ao Instituto de Biociěncias da Universidade de Säo Paulo, para a obtenčäo do título de Mestre em Ciěncias, na Área da Botânica. 87 pp.

  • Nedunchezhian N, Kulandaivelu G (1991) Evidence for the ultraviolet-B (280--320) radiation induced structural reorganization and damage photosystem II polypeptides in isolated chloroplasts. Physiol. Plantarum. 81: 558--562.

    CAS  Article  Google Scholar 

  • Poppe F, Schmidt RA, Hanelt D, Wiencke C (2003) Effects of UV radiation on the ultrastructure of several red algae. Phycol. Res. 51:11--19.

    Google Scholar 

  • Renger G, Völker M, Eckert H, Fromme R, Hohm-Veit S, Gräber P (1989) On the mechanism of photosysthem II deterioration by UVB irradiation. Photochem. Photobiol. 49: 97--105.

    CAS  Article  Google Scholar 

  • Romo H, Paula EJ (1995) Métodos Experimentales para el cultivo de Porphyra. Alveal En K, Ferrario M, Oliveiray E, Sar E (eds.), Manual de Métodos Ficológicos, Universidad de Concepción, Editorial Anibal Pinto S.A., Chile, pp. 55--562.

    Google Scholar 

  • Seckmeyer G, McKenzie R.L (1992) Elevated ultraviolet radiation in New Zealand (45°S) contrasted with Germany (45°S). Nature 359: 135--137.

    Article  Google Scholar 

  • Spjotvoll E, Stoline MR (1973) An extension of the T-method of multiple comparison to include the cases with unequal sample sizes. J. Am. Stat. Assoc. 68: 976--978.

    Article  Google Scholar 

  • Teramura A, Sullivan J (1991). Potential impacts of increased solar UVB on global plant productivity. In Riklis E (ed), Photobiology, Plenum Press New York: 625--634.

    Google Scholar 

  • van de Poll WH, Eggert A, Buma GJ, Breeman AM (2001) Effects of UVB induced DNA damage and photoinhibition on growth of temperate marine red macrophytes: habitat-related differences in UVB tolerance. J. Phycol. 37: 30--37.

    Article  Google Scholar 

  • Vincent W, Roy S (1993) Solar ultraviolet-B radiation and aquatic primary production: Damage, protection, and recovery. Env. Rev. 1: 1--12.

    CAS  Google Scholar 

  • Worrest RC (1983) Impact of solar ultraviolet-B radiation (290--320nm) upon marine microalgae. Physiol. Plantarum 58: 428--434.

    Article  Google Scholar 

  • Yakovleva IM, Titlyanov EA (2001) Effects of high visible and UV irradiance on subtidal Chondrus crispus: Stress, photoinhibition and protective mechanisms. Aquatic Bot. 71: 47--61.

    CAS  Article  Google Scholar 

  • Zar JH (1999). Biostatistical Analysis, 4th Edition, Prentice Hall, Upper Saddle River, NJ, 941 p.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Andrés Mansilla.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Mansilla, A., Werlinger, C., Palacios, M. et al. Effects of UVB Radiation on the Initial Stages of Growth of Gigartina Skottsbergii, Sarcothalia Crispata and Mazzaella Laminarioides (Gigartinales, Rhodophyta). J Appl Phycol 18, 451–459 (2006). https://doi.org/10.1007/s10811-006-9051-9

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10811-006-9051-9

Keywords

  • growth
  • macroalgae
  • Magellanic Region
  • UV