Abstract
Polysaccharide from the green alga Monostroma angicava was extracted with boiling water and was purified by ion-exchange and size-exclusion column chromatography. The radioprotective effect of the polysaccharide was investigated in mice. The results show that polysaccharide from M. angicava has a different chemical composition to other Chlorophyta having a high rhamnose – containing sulfated polysaccharide. The sulfate ester content was estimated to be 21.8%. When the polysaccharide was applied to BALB/c mice following whole-body X-ray irradiation the counts of leukocytes, thrombocytes and erythrocytes recovered more rapidly in the polysaccharide treated mice after irradiation. In the irradiated mice, the polysaccharide significantly increased the spleen index, natural killer cytostatic activity and the transformation response of splenic lymphocytes. The present observations suggest that polysaccharide from M. angicava led to leukocytogensis and hematopoetic activation in mice after irradiation and that the biological response might be caused by immune activation.
Similar content being viewed by others
References
Amornrut C, Toida T, Imanari T, Woo ER, Park H, Linhardt RJ (1999) A new sulfated β-galactan from clams with anti-HIV activity. Carbohydr. Res. 321: 121–127.
Bitter T, Muir HM (1962) A modified uronic acid carbazole reaction. Anal. Biochem. 4: 330–334.
Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 7: 248–254.
Cheung SH, Holland B (1992) Extension of Dennett’s multiple comparison procedure with different simple sizes to the case of several groups. Comput. Stat. Data Anal. 14: 165–182.
Dubois M, Gilles KA, Hamilton JK, Rebers PA, Smith F (1956) Colorrimetric method for determination of sugars and related substances. Anal. Chem. 28: 350–356.
Ellouali M, Boisson-Vidal C, Durand P, Jozefonvicz J (1993) Antitumor activity of low molecular weight fucans extracted from brown seaweed Ascophllum nodosum. Anticancer Res. 13: 2011–2020.
Haroun-Bouhedia F, Ellouali M, Sinquin C, Boisson-Videl C (2000) Relationship between sulfate groups and biological activities of fucans. Thromb. Res. 100: 453–459.
Hayashi K, Hayashi T, Kojima I (1996) AIDS Res. Hum. Retroviruses 12: 1463–1471.
Lahaye M, Jegou D (1993) Chemical and physical-chemical characteristics of dietary fibers from Ulva lactuca (L.) Thuret and Enteromorpha compressa (L.). Grev. J. Appl. Phycol. 5: 195–200.
Maeda M, Uehara T, Harada N, Sekiguchi M, Hiraoka A (1991) Heparinoid-active sulfated polysaccharides from Monostroma nitidum and their distribution in the Chlorophyta. Phytochem. 30: 3611–3614.
Mao WJ, Li BF, Gu QC, Fang YC, Xing HT (2004) Preliminary studies on the chemical characterization and antihyperlipidemic activity of polysaccharide from the brown alga Sargassum fusiforme. Hydrobiologia 512: 263–266.
Moore TA, Zlotnik A (1997) Differential effects of Flk-2/Flt-3 ligand and stem cell factor on murine thymic progenitor cells. J. Immunol. 158: 4187–4192.
Nair CK, Parida DK, Nomura T (2001) Radioprotectors in radiotherapy. J. Radiat. Res. 42: 21–37.
Nishimura Y, Kim HS, Ikota N, Arima H, Bom HS, Kim YH (2003) Radioprotective effect of chitosan in sub-lethally X-ray irradiated mice. J. Radiat. Res. 44: 53–58.
Patchen ML, DiLuzio NR, Jacques P, MacVittie TJ (1984) Soluble polyglycans enhance recovery from cobalt-60-induced hemopoietic injury. J. Biol. Response Mod. 3: 627–633.
Prasada Rao NVSAV, Sastry KV, Venkata Rao E (1984) Carbohydrates of Padina tetrastromatica. Phytochemistry 23: 2531–2533.
Ray B, Lahaye M (1995) Cell-wall polysaccharides from the marine green alga Ulva “rigida” (Ulvales, Chlorophyta). Extraction and chemical composition. Carbohydr. Res. 274: 251–261.
Ruperez P, Ahrazem O, Lea JA (2002) Potential antioxidant capacity of sulfated polysaccharides from the edible marine brown seaweed Fucus vesiculosus. J. Agric. Food Chem. 50: 840–845.
Song JY, Han SK, Bae KG, Lim DS, Son SJ, Jung IS (2003) Radioprotective effects of ginsan, an immunomodulator. Radiat. Res.159: 768–774.
Therho TT, Hartiala K (1971) Method for determination of the sulfate content of glycosamino glycans. Anal. Biochem. 41: 471–476.
Walker C, Canfield PJ, Love DN (1994) Analysis of leucocytes and lymphocyte subsets for different clinical stages of naturally acquired feline immunodeficiency virus infection. Vet Immunol. Immunopathol. 44: 1–12.
Xu SY, Bian RL, Chen X (2003) Pharmacology Experiment Methods, Renmin Hygiene Press, Beijing, pp. 1420.
Xue C, Fang Y, Lin H, Chen L, Li Z, Deng D (2001) Chemical characters and antioxidative properties of sulfated polysaccharides from Laminaria japonica. J. Appl. Phycol. 13: 67–70.
Yashizawa Y, Ametani A, Tsunehiro J, Numura K, Itoh M, Fukui F (1995) Macrophage stimulation activity of the polysaccharide fraction from a marine algae (Porphyra yezoensis): Structure-function relationships and improved solubility. Biosci. Biotech. Biochem. 59: 1933–1937.
Yu P, Zhang Q, Li N, Xu Z, Wang Y, Li Z (2003) Polysaccharides from Ulva pertusa (Chlorophyta) and preliminary studies on their antihyperlipidemia activity. J. Appl. Phycol. 15: 21–27.
Zhang Q, Yu P, Li Z, Zhang H, Xu Z, Li P (2003) Antioxidant activities of sulfated polysaccharide fractions from Porphyra haitanesis. J. Appl. Phycol. 15: 305–310.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Mao, W., Li, Y., Wu, L. et al. Chemical characterization and radioprotective effect of polysaccharide from Monostroma angicava (Chlorophyta). J Appl Phycol 17, 349–354 (2005). https://doi.org/10.1007/s10811-005-8360-8
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/s10811-005-8360-8