Abstract
The effects of temperature (20, 24 and 28 °C) and irradiance (15 and 40 μmol photon m−2 s−1) on the nitrate and ammonium uptake rates of the subtropical red alga, Laurencia brongniartii, were investigated to prepare for tank cultivation. Nitrate uptake followed saturation kinetics and was faster at higher irradiances and temperatures. In contrast, ammonium uptake was linear over the experimental range and was not affected by an increase in temperature. A parameter, β, was calculated to compare substrate uptake rates of nitrate along the linear portion of the uptake curve with that of ammonium. For nitrate, β was lower at low irradiance and higher at high irradiance (β = 0.007 ± 0.003 and 0.030 ± 0.002 [μmol N L−1 (μmol N gww−1 d−)−1], respectively). However, β was 0.023 ± 0.002 and 0.034 ± 0.002 [μmol N L−1 (μmol N gww−1 d−1)−1] for ammonium, suggesting a preference for ammonium over nitrate.
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References
Abe T, Masuda M (1998) Laurencia japonensis sp. nov. (Ceramiales, Rhodophyta). Eur. J. Phycol. 33: 17–24.
Abe T, Masuda M, Kawaguchi S, Kamura S (1998) Taxonomic notes on Laurencia brongniartii (Rhodomelaceae, Rhodophyta). Phycol. Res. 46: 231–237.
Ahn O, Petrell RJ, Harrison PJ (1998) Ammonium and nitrate uptake by Laminaria saccharina and Nereocystis luetkeana originating from a salmon sea cage farm. J. Appl. Phycol. 10: 333–340.
American Water Works Association (1998a) Nitrogen (ammonia) (4500-NH3)/phenate method. In Clesceri LS, Greenberg AE, Eaton AD (eds), Standard Methods for the Examination of Water and Wastewater. Maryland Composition Co., Glen Burnie, Maryland, USA, pp. 4-108–4-109.
American Water Works Association (1998b) Nitrogen (nitrate) (4500-NO3−)/cadmium reduction method. In Clesceri LS, Greenberg AE, Eaton AD (eds), Standard Methods for the Examination of Water and Wastewater. Maryland Composition Co., Glen Burnie, Maryland, USA, pp. 4-117–4-119.
American Water Works Association (1998c) Nitrogen (nitrite) (4500-NO2−)/colorimetric method. In Clesceri LS, Greenberg AE, Eaton AD (eds), Standard Methods for the Examination of Water and Wastewater. Maryland Composition Co., Glen Burnie, Maryland, USA, pp. 4-112–4-114.
Brumfitt W, Hamilton-Miller JMT (1990) The world-wide problem of methicillin-resistant Staphylococcus aureus. Drugs Und. Exp. Clin. Res. 16: 205–214.
D'Elia CF, DeBoer JA (1978) Nutritional studies of two red algae. II. Kinetics of ammonium and nitrate uptake. J. Phycol. 14: 266–272.
Erickson KL (1983) Constituents of Laurencia. In Sheuer PJ (ed), Marine Natural Products, Vol. 5. Academic Press, New York, pp. 131–257.
Fenical W (1975) Halogenation in the Rhodophyta. A review. J. Phycol. 11: 245–259.
Fenical W, Norris JN (1975) Chemotaxonomy in marine algae: Chemical separation of some Laurencia species (Rhodophyta) from the Gulf of California. J. Phycol. 11: 104–108.
Fitzgerald GP, Nelson TC (1966) Extractive and enzymatic analyses for limiting or surplus phosphorus in algae. J. Phycol. 2: 32–37.
Friedlander M, Dawes CJ (1985) In situ uptake kinetics of ammonium and phosphate and chemical composition of the red seaweed Gracilaria tikvahiae McLachlan (Rhodophyta). J. Phycol. 21: 448–453.
Fujii MT, Cordeiro-Marino M (1996) Laurencia translucida sp. nov. (Ceramiales, Rhodophyta) form Brazil. Phycologia 35: 542– 549.
Fujita RM (1985) The role of nitrogen status in regulating transient ammonium uptake and nitrogen storage by macroalgae. J. Exp. Mar. Biol. Ecol. 92: 283–301.
Graham LE, Wilcox LW (2000) Algae. Prentice Hall, New Jersey, 640 pp.
Hackett HE (1974) Laurencia brongniartii (Rhodophyta–Rhodomelaceae) from Florida. Flor. Sci. 37: 50–52.
Haines KC, Wheeler PA (1978) Ammonium and nitrate uptake by the marine macrophytes Hypnea musciformis (Rhodophyta) and Macrocystis pyrifera (Phaeophyta). J. Phycol. 14: 319– 324.
Harada H, Noro T, Kamei Y (1996a) Cytotoxic spectra of selective antitumor extracts from marine algae extracts to several tumor cells lines. Mar. High. Biosci. Cntr. Rep. 4: 23–26.
Harada H, Noro T, Kamei Y (1996b) Effect of in vitro selective antitumor active extracts from marine algae on proliferations of murine tumor L1210 and normal NIH-3T3 cells. Mar. High. Biosci. Cntr. Rep. 4: 27–31.
Harada H, Noro T, Kamei Y (1997) Selective antitumor activity in vitro from marine algae from Japan coasts. Biol. Pharm. Bull. 20: 541–546.
Harrison PJ, Parslow JS, Conway HL (1989) Determination of nutrient uptake kinetic parameters: A comparison of methods. Mar. Ecol. Prog. Ser. 52: 301–312.
Horikawa M, Noro T, Kamei Y (1995) Screening of antibacterial activity from marine algae of Kyushu Island, Japan: II. Mar. High. Biosci. Cntr. Rep. 2: 43–48.
Horikawa M, Noro T, Kamei Y (1999) In vitro anti-methicillin-resistant Staphylococcus aureus activity found in extracts of marine algae indigenous to the coastline of Japan. J. Antibiot. 52: 186–189.
Kamei Y, Noro T, Yamaguchi Y (1995) Screening of antiviral activity from marine algae: I. Mar. High. Biosci. Cntr. Rep. 2: 49–56.
Lembi CA, Waaland JR (1988) Algae and Human Affairs. Cambridge University Press, New York, 590 pp.
McDermid KJ (1988) Section V. Laurencia (Rhodophyta, Rhodomelaceae) introduction. In Abbot IA (ed), Taxonomy of Economic Seaweeds with Reference to Some Pacific and Caribbean Species. La Jolla, California, Sea Grant College Program, Vol. 2, pp. 221–229.
Nishihara GN, Mori Y, Terada R, Noro T (2004a) A simplified method to isolate and cultivate, Laurencia brongniartii (Rhodophyta, Ceramiales) from Kagoshima, Japan. Suisanzoshoku 52: 1–10.
Nishihara GN, Mori Y, Terada R, Noro T (2004b) Habitat characteristics and seasonal growth of Laurencia brongniartii (Ceramiales, Rhodophyta) in Kagoshima, Southern Japan. Phycol. Res. 52: 30–37.
Pratt R, Daniels TC, Gunnison JB, Kumler WD, Oneto JF, Strait LA (1944) Chlorellin, an antibacterial substance from Chlorella. Science 99: 351–352.
Schwartz R, Hirsch C, Flor J, Baroni A, Socal G (1990) Pharmaceuticals from cultured algae. J. Ind. Microbiol. 5: 113–124.
Saito Y (1967) Studies on Japanese species of Laurencia, with special reference to their comparative morphology. Mem. Fac. Fish. Hokkaido Univ. 15: 1–81.
Saito Y, Takata A (1974) On Laurencia brongniartii from Japan. Jpn. J. Phycol. 22: 83–89.
Shepherd SA, Womersley HBS (1970) The sublittoral ecology of West Island, South Australia: 1. Environmental features and the algal ecology. Trans. R. Soc. S. Aust. 94: 105–138.
Shepherd SA, Womersley HBS (1971) Pearson Island Expedition 1969: 7. The subtidal ecology of benthic algae. Trans. R. Soc. S. Aust. 95: 155–167.
Smit AJ (2002) Nitrogen uptake by Gracilaria gracilis (Rhodophyta): Adaptations to a temporally variable nitrogen environment. Bot. Mar. 45: 196–209.
Topinka JA (1978) Nitrogen uptake by Fucus spiralis (Phaeophyceae). J. Phycol. 14: 247–247.
Wheeler WN (1982) Nitrogen nutrition of Macrocystis. In Srivastava LM (ed), Synthetic Degradative Processes in Marine Macrophytes. Walter de Gruyter, Berlin, pp. 121–137.
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Nishihara, G.N., Terada, R. & Noro, T. Effect of temperature and irradiance on the uptake of ammonium and nitrate by Laurencia brongniartii (Rhodophyta, Ceramiales). J Appl Phycol 17, 371–377 (2005). https://doi.org/10.1007/s10811-005-5519-2
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DOI: https://doi.org/10.1007/s10811-005-5519-2