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Bioelectrochemical assessment of a novel electrogenic Bacillus altitudinis AC11.2 for electricity generation in microbial fuel cell (MFC) system

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Abstract

Electrogen is a microbial group that plays an important role as anodic biocatalyst of microbial fuel cells (MFCs), one of the most extensively studied microbial-based technologies for bioelectricity generation. The aims of this research were to isolate potential electrogenic bacteria from aquaculture pond sediments and conduct a thorough evaluation on the MFCs’ electricity production and efficiency parameters. A total of 18 electrogenic bacteria having various electrochemical abilities was successfully isolated using thioglycollate solid media enriched with Fe3+. Five isolates (namely KCf1, KCf2, KCf4, KCf10, and KCf14) were non-pathogenic electrogens and able to produce relatively stable and high open-circuit voltage values (690–810 mV) on glucose-fed MFCs. Further evaluations on electricity production in close-circuit mode (fixed resistor technique, polarization tests, and analysis of power overshoot phenomenon) and efficiency parameters (Coulombic and energy efficiencies) showed that KCf2, molecular identified as Bacillus altitudinis AC11.2, was the most potential MFC biocatalyst among all isolates. It produced an MPP value of 67.11 mW m−2, current density of 333.03 mA m−2, and Coulombic and energy efficiency of 53.86% and 63.27%, respectively. Efforts to increase the MFC’s electrical output have been done by assembling four reactors in series and parallel circuits, obtaining the maximum total voltage of 1.6–2.0 V (for series configuration). This potential output was higher than a portable zinc–carbon battery (1.5 V) and a Ni–Cd battery (1.2 V). However, the voltage reversal suffered in series circuits was another challenge in the development of MFCs for bioelectricity production, since the existence of this phenomenon due to biological factors (microbial metabolism dynamics) are not easy to be controlled.

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Acknowledgements

The authors would like to thank the Ministry of Education, Culture, Research and Technology of the Republic of Indonesia for providing the funding for this research through the accelerated master’s program leading to doctorate (PMDSU) research grant number 1471/IT3.11/PN/2018.

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Authors and Affiliations

  1. Study Program of Soil Sciences, Faculty of Agriculture, IPB University, 16680, Bogor, Indonesia

    Yohanna Anisa Indriyani

  2. Department of Biology, Faculty of Mathematics and Natural Sciences, IPB University, 16680, Bogor, Indonesia

    Iman Rusmana

  3. Department of Soil Science and Land Resource, Faculty of Agriculture, IPB University, 16680, Bogor, Indonesia

    Syaiful Anwar, Gunawan Djajakirana & Dwi Andreas Santosa

  4. Biotechnology Center, Research and Community Empowerment Institute, IPB University, 16680, Bogor, Indonesia

    Dwi Andreas Santosa

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  1. Yohanna Anisa Indriyani
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Contributions

YAI, IR, SA, GD, and DAS designed the study. YAI carried out the laboratory work, analyzed the data, and wrote the original draft. DAS, GD, SA, and IR supervised the study and revised the final version of the document. YAI created the manuscript’s illustration and edited the final version. All authors read and approved the final version of the manuscript.

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Correspondence to Dwi Andreas Santosa.

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Indriyani, Y.A., Rusmana, I., Anwar, S. et al. Bioelectrochemical assessment of a novel electrogenic Bacillus altitudinis AC11.2 for electricity generation in microbial fuel cell (MFC) system. J Appl Electrochem 54, 977–997 (2024). https://doi.org/10.1007/s10800-023-02020-9

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