Electrochemical detection of Flutamide as an anticancer drug with gold nanoparticles modified glassy carbon electrode in the presence of prostate cancer cells


In the current study, a novel electrochemical sensor was constructed based on gold nanoparticles modified glassy carbon electrode (AuNPs/GCE) to detect Flutamide. Field emission scanning electron microscope was used to identify the morphology of the modified electrode. Cyclic voltammetry and differential pulse voltammetry (DPV) were used to investigate the electrochemical activity of the modified electrode. Flutamide concentration, pH, and scan rate were optimized as electrochemical parameters. Flutamide peak currents obtained by DPV were linear within the concentration range of 1–600 µM, and the detection limit was calculated as 1.5 nM. In addition, the fabricated electrochemical sensor was successfully employed to detect Flutamide in a cell culture media containing prostate cancer cells as the biological sample. The stability of the modified electrode within a day showed an appropriate precision.

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  1. 1.

    Temerk YM, Ibrahim HS, Schuhmann W (2016) Square wave cathodic adsorptive stripping voltammetric determination of the anticancer drugs flutamide and irinotecan in biological fluids using renewable pencil graphite electrodes. Electroanalysis 28(2):372–379

    CAS  Google Scholar 

  2. 2.

    Temerk Y, Ibrahim H (2015) Electrochemical studies and spectroscopic investigations on the interaction of an anticancer drug flutamide with DNA and its analytical applications. J Electroanal Chem 736:1–7

    CAS  Google Scholar 

  3. 3.

    Ahmadi F et al (2015) Synthesis of Ag nanoparticles for the electrochemical detection of anticancer drug flutamide. Chin J Catal 36(3):439–445

    CAS  Google Scholar 

  4. 4.

    Brahman PK et al (2012) Voltammetric determination of anticancer drug flutamide in surfactant media at polymer film modified carbon paste electrode. Colloids Surf A 396:8–15

    CAS  Google Scholar 

  5. 5.

    Rezaeifar Z et al (2018) Electrochemical determination of anticancer drug, flutamide in human plasma sample using a microfabricated sensor based on hyperbranchedpolyglycerol modified graphene oxide reinforced hollow fiber-pencil graphite electrode. Mater Sci Eng C 91:10–18

    CAS  Google Scholar 

  6. 6.

    Karthik R et al (2017) A facile graphene oxide based sensor for electrochemical detection of prostate anti-cancer (anti-testosterone) drug flutamide in biological samples. RSC Adv 7(41):25702–25709

    CAS  Google Scholar 

  7. 7.

    Neri R (1989) Pharmacology and pharmacokinetics of flutamide. Urology 34(4): 19–21

    CAS  PubMed  Google Scholar 

  8. 8.

    Kakutani N et al (2020) Evaluation of covalent binding of flutamide and its risk assessment using 19F-NMR. Xenobiotica 51:88–94

    PubMed  Google Scholar 

  9. 9.

    Núñez-Vergara LJ et al (2001) An electrochemical evidence of free radicals formation from flutamide and its reactivity with endo/xenobiotics of pharmacological relevance. Bioelectrochem 53(1):103–110

    Google Scholar 

  10. 10.

    Sufrin G, Coffey D (1976) Flutamide. Mechanism of action of a new nonsteroidal antiandrogen. Invest Urol 13(6):429–434

    CAS  PubMed  Google Scholar 

  11. 11.

    Johnson DB, Sonthalia S (2019) Flutamide. StatPearls [Internet]. StatPearls Publishing, Treasure Island

    Google Scholar 

  12. 12.

    Álvarez Lueje A et al (1998) Electrochemical study of flutamide, an anticancer drug, and its polarographic, UV spectrophotometric and HPLC determination in tablets. Electroanal Int J Devoted Fundam Pract Asp Electroanal 10(15):1043–1051

    Google Scholar 

  13. 13.

    Ensafi AA, Khoddami E, Rezaei B (2016) Development of a cleanup and electrochemical determination of flutamide using silica thin film pencil graphite electrode functionalized with thiol groups. J Iran Chem Soc 13(9):1683–1690

    CAS  Google Scholar 

  14. 14.

    Mutharani B, Ranganathan P, Chen S-M (2019) Chitosan-gold collapse gel/poly (bromophenol blue) redox-active film. A perspective for selective electrochemical sensing of flutamide. Int J Biol Macromol 124:759–770

    CAS  PubMed  Google Scholar 

  15. 15.

    El-Shanawany A et al (2014) Electrochemical characterization and determination of the anticancer drug, flutamide by cyclic voltammetry. Ann Chem Forsch 2:29–40

    CAS  Google Scholar 

  16. 16.

    Pecková K et al (2012) Voltammetric determination of flutamide and its metabolite 4-nitro-3-trifluoromethylaniline at a hanging mercury drop minielectrode. Collect Czech Chem Commun 76(12):1811–1823

    Google Scholar 

  17. 17.

    Zokhtareh R, Rahimnejad M (2018) A novel sensitive electrochemical sensor based on nickel chloride solution modified glassy carbon electrode for curcumin determination. Electroanalysis 30(5):921–927

    CAS  Google Scholar 

  18. 18.

    Ezoji H, Rahimnejad M (2016) Electrochemical determination of bisphenol A on multi-walled carbon nanotube/titanium dioxide modified carbon paste electrode. Int J Sci Eng Res 7(6):242–246

    Google Scholar 

  19. 19.

    Bard AJ et al (1980) Electrochemical methods: fundamentals and applications, vol 2. Wiley, New York

    Google Scholar 

  20. 20.

    Yamazoe N (2005) Toward innovations of gas sensor technology. Sens Actuators B 108(1–2):2–14

    CAS  Google Scholar 

  21. 21.

    Wilson DM et al (2001) Chemical sensors for portable, handheld field instruments. IEEE Sens 1(4):256–274

    CAS  Google Scholar 

  22. 22.

    Brett CM (2001) Electrochemical sensors for environmental monitoring. Strategy and examples. Pure Appl Chem 73(12):1969–1977

    CAS  Google Scholar 

  23. 23.

    Wang J (1991) Modified electrodes for electrochemical sensors. Electroanalysis 3(4–5):255–259

    CAS  Google Scholar 

  24. 24.

    Murray RW, Ewing AG, Durst RA (1987) Chemically modified electrodes. Molecular design for electroanalysis. Anal Chem 59(5):379A-390A

    CAS  PubMed  Google Scholar 

  25. 25.

    Maduraiveeran G, Sasidharan M, Ganesan V (2018) Electrochemical sensor and biosensor platforms based on advanced nanomaterials for biological and biomedical applications. Biosens Bioelectron 103:113–129

    CAS  PubMed  Google Scholar 

  26. 26.

    Luo X et al (2006) Application of nanoparticles in electrochemical sensors and biosensors. Electroanalysis 18(4):319–326

    CAS  Google Scholar 

  27. 27.

    Zen JM, Senthil Kumar A, Tsai DM (2003) Recent updates of chemically modified electrodes in analytical chemistry. Electroanalysis 15(13):1073–1087

    CAS  Google Scholar 

  28. 28.

    Moniruzzaman M, Winey KI (2006) Polymer nanocomposites containing carbon nanotubes. Macromol 39(16):5194–5205

    CAS  Google Scholar 

  29. 29.

    Durst R (1997) Chemically modified electrodes: recommended terminology and definitions (IUPAC Recommendations 1997). Pure Appl Chem 69(6):1317–1324

    CAS  Google Scholar 

  30. 30.

    Kumar S et al (2015) Graphene, carbon nanotubes, zinc oxide and gold as elite nanomaterials for fabrication of biosensors for healthcare. Biosens Bioelectron 70:498–503

    CAS  PubMed  Google Scholar 

  31. 31.

    Dékány I (2011) Titanium dioxide and gold nanoparicles for environmental and biological application. Ann Faculty Eng Hunedoara 9(1):161

    Google Scholar 

  32. 32.

    Boddu SR et al (2011) Gold, silver, and palladium nanoparticle/nano-agglomerate generation, collection, and characterization. J Nanopart Res 13(12):6591–6601

    CAS  Google Scholar 

  33. 33.

    Elahi N, Kamali M, Baghersad MH (2018) Recent biomedical applications of gold nanoparticles: a review. Talanta 184:537–556

    CAS  PubMed  Google Scholar 

  34. 34.

    Li G, Miao P (2013) Theoretical background of electrochemical analysis. Electrochemical analysis of proteins and cells. Springer, Berlin, Heidelburg, pp 5–18

    Google Scholar 

  35. 35.

    Laviron E (1979) General expression of the linear potential sweep voltammogram in the case of diffusionless electrochemical systems. J Electroanal Chem Interfacial Electrochem 101(1):19–28

    CAS  Google Scholar 

  36. 36.

    Shetti NP et al (2009) Electrochemical oxidation of loop diuretic furosemide at gold electrode and its analytical applications. Int J Electrochem Sci 4:104–121

    CAS  Google Scholar 

  37. 37.

    Hegde RN et al (2009) Electro-oxidation and determination of gabapentin at gold electrode. J Electroanal Chem 635(1):51–57

    CAS  Google Scholar 

  38. 38.

    Kubendhiran S et al (2018) Innovative strategy based on a novel carbon-black− β-cyclodextrin nanocomposite for the simultaneous determination of the anticancer drug flutamide and the environmental pollutant 4-nitrophenol. Anal chem 90(10):6283–6291

    CAS  PubMed  Google Scholar 

  39. 39.

    Mehrabi A et al (2019) Electrochemical detection of flutamide with gold electrode as an anticancer drug. Biocatal Agricul Biotech 22:101375

    Google Scholar 

  40. 40.

    Ding L et al (2007) A disposable impedance sensor for electrochemical study and monitoring of adhesion and proliferation of K562 leukaemia cells. Electrochem Commun 9(5):953–958

    CAS  Google Scholar 

  41. 41.

    Chen H et al (2005) Detection of Saccharomyces cerevisiae immobilized on self-assembled monolayer (SAM) of alkanethiolate using electrochemical impedance spectroscopy. Anal Chim Acta 554(1–2):52–59

    CAS  Google Scholar 

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This study was funded by Babol Noshirvani University of Technology (Grant No. BNUT/370393/2020).

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Correspondence to Mostafa Rahimnejad.

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Mehrabi, A., Rahimnejad, M., Mohammadi, M. et al. Electrochemical detection of Flutamide as an anticancer drug with gold nanoparticles modified glassy carbon electrode in the presence of prostate cancer cells. J Appl Electrochem 51, 597–606 (2021). https://doi.org/10.1007/s10800-020-01519-9

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  • Glassy carbon electrode
  • Anticancer drug
  • Flutamide
  • Cyclic voltammetry
  • Electrochemical sensor
  • Gold nanoparticles